FastQCFastQC Report
Fri 10 Feb 2017
ERR1631026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200379
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT53890.44894154262945285No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG43560.3628853886980695No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC41560.3462239842583051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39200.32656352701938307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.31356763155636674No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT35340.29440701645063766RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC23190.19318898447906868No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG17330.1443710694705589No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA16070.1338743846735073No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT15700.13079202485215086No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG14840.12362762094305214No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG14830.12354431392085333No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14680.122294708587871No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG14530.12104510325488868No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG13620.11346416423479586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13450.11204794485741587No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC13400.11163140974642174RNA PCR Primer, Index 29 (95% over 21bp)
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT13330.11104826059102998No Hit
TATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATCA12680.10563330414810655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA250.005496438629.624
GGTATCA5450.027.4954151
GTATCAA19650.025.702291
GATTACG1300.025.6153851
CGTCTTC2250.024.66666637
TATACAC10450.023.7224883
CCGTCTT5100.023.57843237
CTTATAC10000.023.4949991
GCCGTCT5300.022.68867736
TTATACA10950.021.963472
TTACGGG1550.021.4838713
AGGACCG705.1022344E-621.1428595
AACGGCC1303.274181E-1119.92307737
TTAACGG1303.274181E-1119.92307735
CGGCGTA609.2371413E-418.512
ACGGTAC2300.018.53
TGCCGTC6900.017.96376835
TAACGGC1502.5102054E-1017.26666636
CGAACGC650.001579989417.07692330
TCACGGT2500.017.021
Produced by FastQC (version 0.11.5)