##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631026.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200379
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.457322229062655	31.0	31.0	34.0	28.0	34.0
2	31.440867426037943	31.0	31.0	34.0	28.0	34.0
3	30.857160113597455	31.0	31.0	34.0	26.0	34.0
4	35.021853931133414	37.0	35.0	37.0	32.0	37.0
5	35.264854683395825	37.0	35.0	37.0	33.0	37.0
6	35.461084374185155	37.0	35.0	37.0	33.0	37.0
7	35.48592652820484	37.0	35.0	37.0	33.0	37.0
8	35.5071606550931	37.0	35.0	37.0	33.0	37.0
9	37.2738026906502	39.0	37.0	39.0	34.0	39.0
10	37.116738963277434	39.0	37.0	39.0	33.0	39.0
11	37.1835062092889	39.0	37.0	39.0	33.0	39.0
12	37.1266641619022	39.0	37.0	39.0	33.0	39.0
13	37.18609955688995	39.0	37.0	39.0	33.0	39.0
14	38.34360314533993	40.0	38.0	41.0	34.0	41.0
15	38.35149232034216	40.0	38.0	41.0	34.0	41.0
16	38.36141501975626	40.0	38.0	41.0	34.0	41.0
17	38.33751090280653	40.0	38.0	41.0	34.0	41.0
18	38.34218859210299	40.0	38.0	41.0	34.0	41.0
19	38.38190938028739	40.0	38.0	41.0	34.0	41.0
20	38.36208647435518	40.0	38.0	41.0	34.0	41.0
21	38.32062123712594	40.0	38.0	41.0	34.0	41.0
22	38.27303793218642	40.0	38.0	41.0	34.0	41.0
23	38.24564075179589	40.0	38.0	41.0	34.0	41.0
24	38.22598945832941	40.0	38.0	41.0	34.0	41.0
25	38.20376397787699	40.0	38.0	41.0	34.0	41.0
26	38.07912001126311	40.0	37.0	41.0	33.0	41.0
27	38.03726989559131	40.0	37.0	41.0	33.0	41.0
28	37.9892667232599	40.0	37.0	41.0	33.0	41.0
29	37.908405595232836	40.0	37.0	41.0	33.0	41.0
30	37.823915613318796	40.0	37.0	41.0	33.0	41.0
31	37.80231160325197	40.0	37.0	41.0	33.0	41.0
32	37.74768468958554	40.0	37.0	41.0	33.0	41.0
33	37.707678158315	40.0	37.0	41.0	33.0	41.0
34	37.637716921072425	40.0	37.0	41.0	32.0	41.0
35	37.58352903541298	40.0	37.0	41.0	32.0	41.0
36	37.54785446929678	40.0	37.0	41.0	32.0	41.0
37	37.47699601542513	40.0	37.0	41.0	32.0	41.0
38	37.42832721998636	39.0	36.0	41.0	32.0	41.0
39	37.36711655235555	39.0	36.0	41.0	31.0	41.0
40	37.3152321058599	39.0	36.0	41.0	31.0	41.0
41	37.22238476347887	39.0	36.0	41.0	31.0	41.0
42	37.16882168048591	39.0	36.0	41.0	31.0	41.0
43	36.5780057798412	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	8.0
12	1.0
13	1.0
14	6.0
15	2.0
16	4.0
17	11.0
18	17.0
19	45.0
20	93.0
21	234.0
22	429.0
23	841.0
24	1489.0
25	2534.0
26	4103.0
27	6497.0
28	9778.0
29	13785.0
30	19220.0
31	26060.0
32	34103.0
33	44670.0
34	59402.0
35	80937.0
36	112038.0
37	166172.0
38	290118.0
39	327770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48354727965084	20.795432109358796	14.54024104053803	24.180779570452334
2	14.956776151532141	21.79503306872246	40.32101527934094	22.927175500404456
3	15.419963194957592	27.663179712407498	32.67201442211169	24.244842670523227
4	12.671997760707242	17.720736534044665	39.30100409953856	30.30626160570953
5	13.129020084489982	38.32656186087894	35.0970818383194	13.447336216311681
6	29.234183537032887	39.45453894145099	17.652341468819433	13.65893605269669
7	26.29127967083729	32.11885579471151	23.58396806341997	18.00589647103123
8	25.348244179546626	35.15089817465984	21.270781978025273	18.230075667768265
9	25.786938958445628	15.234021921409822	20.12730979132424	38.85172932882032
10	15.694709754169308	28.204092207544452	33.28432103527303	22.816877003013214
11	32.73616082920478	23.773991381055485	24.727273636076603	18.762574153663135
12	22.66392531025618	25.58208699085872	29.930130400481847	21.823857298403254
13	27.945007368506115	21.1628993842778	26.940407987810516	23.951685259405572
14	22.055700741182577	20.963795601222614	27.692253863154885	29.288249794439924
15	23.77774019705443	28.412776298152497	24.236345354258944	23.573138150534124
16	23.363870910770682	27.22881689866284	24.541748897639827	24.86556329292665
17	22.15658554506535	28.030980215415298	26.110836660754643	23.70159757876471
18	23.405607728892292	26.264038274578276	27.186580238408038	23.143773758121394
19	24.658128807651583	26.03894270059706	26.920580916527197	22.38234757522416
20	23.962848400380214	26.226133579477818	26.891673379824205	22.919344640317764
21	24.479851780146102	26.174399918692348	26.498547542067964	22.847200759093585
22	23.36620350739225	27.33978185223167	26.676158113395854	22.61785652698023
23	23.84621856930186	25.914898544543014	26.7221435896496	23.51673929650552
24	23.348792339752695	26.662245840688648	26.63650397082921	23.352457848729443
25	23.552311394984418	26.37933519330145	27.38085221417569	22.68750119753844
26	23.736503221066013	26.65249891909139	27.275385524071982	22.33561233577062
27	23.983425234863322	26.653415296335574	26.2553743442696	23.107785124531503
28	22.889187498281792	25.95655205564243	27.51597620418218	23.6382842418936
29	23.642282978959145	26.020865076779913	27.494399685432686	22.842452258828253
30	23.97651075202082	26.0219480680685	27.455911841176828	22.54562933873385
31	23.436181406039257	25.912899176010246	26.686738105215102	23.964181312735395
32	22.520220696963207	26.564276782582834	27.671010572494186	23.24449194795977
33	23.494912856689428	26.79228810234101	26.99880621037189	22.71399283059767
34	23.22533133285404	26.687987710548082	26.922247056971173	23.1644338996267
35	23.497412067355395	26.231798456987338	28.230083998470484	22.04070547718679
36	24.23542897701476	26.99772321908331	25.517857276743428	23.248990527158504
37	23.34362730437637	26.319354137318296	27.038210431871935	23.2988081264334
38	24.080811143813747	25.774276291071402	26.953320576251333	23.191591988863518
39	23.16101831171655	26.22588365841122	27.12326690153693	23.4898311283353
40	23.159185557228177	25.60841200987355	28.374121839852247	22.85828059304603
41	23.005567408293548	25.389647769579444	27.419839900564742	24.184944921562273
42	22.776389790224588	26.265621108000058	27.29796172708786	23.660027374687495
43	23.562391544670476	24.50634341320533	28.269238298903932	23.662026743220267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	27.5
2	37.0
3	135.5
4	234.0
5	234.0
6	268.0
7	302.0
8	333.0
9	364.0
10	555.0
11	746.0
12	746.0
13	1140.0
14	1534.0
15	3818.0
16	6102.0
17	6792.0
18	7482.0
19	7482.0
20	7486.5
21	7491.0
22	8874.5
23	10258.0
24	13690.5
25	17123.0
26	17123.0
27	21469.5
28	25816.0
29	30922.0
30	36028.0
31	42477.5
32	48927.0
33	48927.0
34	55549.5
35	62172.0
36	67306.0
37	72440.0
38	77905.5
39	83371.0
40	83371.0
41	85469.5
42	87568.0
43	88977.0
44	90386.0
45	89847.5
46	89309.0
47	89309.0
48	87438.0
49	85567.0
50	88402.0
51	91237.0
52	83906.5
53	76576.0
54	76576.0
55	79589.0
56	82602.0
57	73181.0
58	63760.0
59	52820.5
60	41881.0
61	41881.0
62	39153.5
63	36426.0
64	28770.5
65	21115.0
66	16982.5
67	12850.0
68	12850.0
69	10962.5
70	9075.0
71	8215.5
72	7356.0
73	9443.5
74	11531.0
75	11531.0
76	10168.5
77	8806.0
78	5982.5
79	3159.0
80	1814.5
81	470.0
82	470.0
83	313.0
84	156.0
85	110.5
86	65.0
87	46.0
88	27.0
89	27.0
90	17.5
91	8.0
92	5.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1200379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.33027940853511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.46131941517719	32.26429775387808
2	14.19226038975504	12.299092162535425
3	4.707330372186397	6.119098208853605
4	2.0656683593295018	3.5802394870047043
5	1.0987032464376292	2.380355932760354
6	0.6642142825462808	1.726835426992202
7	0.4266329292517594	1.294028682055237
8	0.29925417078466526	1.0373413467415225
9	0.23005995659753609	0.8971705980078027
>10	1.492400622300576	12.848261215198045
>50	0.2044259942518053	6.211822305281952
>100	0.14230431963392443	11.903302775796822
>500	0.010219686407848495	2.9457680345549986
>1k	0.005013431068001148	4.042128137497128
>5k	1.92824271846198E-4	0.4502579328421841
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	5389	0.44894154262945285	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	4356	0.3628853886980695	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4156	0.3462239842583051	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3920	0.32656352701938307	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3764	0.31356763155636674	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	3534	0.29440701645063766	RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	2319	0.19318898447906868	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	1733	0.1443710694705589	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	1607	0.1338743846735073	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1570	0.13079202485215086	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1484	0.12362762094305214	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1483	0.12354431392085333	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1468	0.122294708587871	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1453	0.12104510325488868	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	1362	0.11346416423479586	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1345	0.11204794485741587	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	1340	0.11163140974642174	RNA PCR Primer, Index 29 (95% over 21bp)
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1333	0.11104826059102998	No Hit
TATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATCA	1268	0.10563330414810655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.33070221988222E-5	0.0	0.0	0.0	0.0
8	8.33070221988222E-5	0.0	0.0	0.0	0.0
9	8.33070221988222E-5	0.0	0.0	0.0	0.0
10	8.33070221988222E-5	0.0	0.0	0.0	0.0
11	8.33070221988222E-5	0.0	0.0	0.0	0.0
12	8.33070221988222E-5	0.0	0.0	0.0	0.0
13	8.33070221988222E-5	0.0	0.0	0.0	0.0
14	8.33070221988222E-5	0.0	0.0	0.0	0.0
15	3.332280887952888E-4	0.0	0.0	8.33070221988222E-5	0.0
16	4.1653511099411103E-4	0.0	0.0	1.666140443976444E-4	0.0
17	5.831491553917555E-4	0.0	0.0	1.666140443976444E-4	0.0
18	5.831491553917555E-4	0.0	0.0	3.332280887952888E-4	0.0
19	5.831491553917555E-4	0.0	0.0	4.1653511099411103E-4	0.0
20	5.831491553917555E-4	0.0	0.0	4.1653511099411103E-4	0.0
21	5.831491553917555E-4	0.0	0.0	4.1653511099411103E-4	0.0
22	6.664561775905776E-4	0.0	0.0	4.998421331929333E-4	0.0
23	6.664561775905776E-4	0.0	0.0	9.996842663858665E-4	0.0
24	7.497631997893998E-4	0.0	0.0	0.0016661404439764441	0.0
25	7.497631997893998E-4	0.0	0.0	0.0018327544883740886	0.0
26	7.497631997893998E-4	0.0	0.0	0.002082675554970555	0.0
27	9.996842663858665E-4	0.0	0.0	0.0031656668435552436	0.0
28	0.0013329123551811552	0.0	0.0	0.011079833952443354	0.0
29	0.0013329123551811552	0.0	0.0	0.028657615636394837	0.0
30	0.0013329123551811552	0.0	0.0	0.05231680994086035	0.0
31	0.0013329123551811552	0.0	0.0	0.11263109401280762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	25	0.0054964386	29.6	24
GGTATCA	545	0.0	27.495415	1
GTATCAA	1965	0.0	25.70229	1
GATTACG	130	0.0	25.615385	1
CGTCTTC	225	0.0	24.666666	37
TATACAC	1045	0.0	23.722488	3
CCGTCTT	510	0.0	23.578432	37
CTTATAC	1000	0.0	23.494999	1
GCCGTCT	530	0.0	22.688677	36
TTATACA	1095	0.0	21.96347	2
TTACGGG	155	0.0	21.483871	3
AGGACCG	70	5.1022344E-6	21.142859	5
AACGGCC	130	3.274181E-11	19.923077	37
TTAACGG	130	3.274181E-11	19.923077	35
CGGCGTA	60	9.2371413E-4	18.5	12
ACGGTAC	230	0.0	18.5	3
TGCCGTC	690	0.0	17.963768	35
TAACGGC	150	2.5102054E-10	17.266666	36
CGAACGC	65	0.0015799894	17.076923	30
TCACGGT	250	0.0	17.02	1
>>END_MODULE