FastQCFastQC Report
Fri 10 Feb 2017
ERR1631025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216964
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG139186.4148891060268065No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT131576.064139672941133TruSeq Adapter, Index 8 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT119475.506443465275345No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC73743.398720525064066No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC52542.421599896757066RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC39051.7998377611032246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33031.5223723751405764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29151.3435408639221254No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG17860.8231780387529728No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15980.7365277188842388No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA13400.6176139820431039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11800.5438690289633303No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT11230.517597389428661No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10530.48533397245626No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10300.47473313545104256No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9380.43232978743017275No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT9100.4194244206412123No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7650.35259305691266757No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7460.3438358437344444No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6850.3157205803727808No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6690.3083460850648034No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6530.30097158975682603No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5940.2737781383086595No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4780.22031304732582363No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4510.20786858649361187No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4490.20694677458011468No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4330.1995722792721373No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4200.1935805018344057No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4140.1908150660939142No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT4080.18804963035342268TruSeq Adapter, Index 8 (95% over 24bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4010.18482328865618258No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3940.1815969469589425No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3940.1815969469589425No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3930.1811360410021939No Hit
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT3910.18021422908869675TruSeq Adapter, Index 8 (95% over 21bp)
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3770.17376154569421656No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3720.17145701591047363No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3700.17053520399697644No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3650.16823067421323354No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3560.16408252060249628No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3540.1631607086889991No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.15809074316476465No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3420.15762983720801607No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3280.15117715381353589No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3190.14702900020279863No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3140.14472447041905567No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3070.1414981287218156No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3060.14103722276506703No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2790.12859276193285524No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2770.12767095001935805No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2650.12214007853837502No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2620.12075736066812928No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2620.12075736066812928No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2600.11983554875463212No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2570.11845283088438635No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.11707011301414061No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2490.11476558323039766No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA2490.11476558323039766No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2470.11384377131690049No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2450.11292195940340333No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2450.11292195940340333No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2430.11200014748990615No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2410.11107833557640898No Hit
CTGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT2390.11015652366291181No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2360.10877380579266606No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2320.10693018196567172No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2240.10324293431168304No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2210.10186021644143729No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2200.1013993104846887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2190.10093840452794012No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2180.10047749857119152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC200.001838647237.04
GCAACGC200.001838647237.03
TAGCAAC200.001838647237.01
ATTACGG200.001838647237.02
ACTCAGA403.808782E-837.01
TCGCCGA200.001838647237.01
TCAGAGT403.808782E-837.03
AGCAACG200.001838647237.02
TTTACAC200.001838647237.02
CTTATAC13150.035.5931551
TTATACA14050.033.3131682
CTCAGAG451.0731128E-732.888892
CGTCTTC6700.031.7537337
GATACAC352.3794526E-531.7142853
TGATACA352.3794526E-531.7142852
GAGCCGC303.589944E-430.83333424
CGAGCCG303.589944E-430.83333423
GCCGCTC303.589944E-430.83333426
TATACAC15550.030.2186473
AGGACCG250.005486641529.65
Produced by FastQC (version 0.11.5)