##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631025.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216964
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.79990689699674	31.0	30.0	33.0	27.0	34.0
2	30.849970502018767	31.0	31.0	34.0	27.0	34.0
3	30.24961283899633	31.0	30.0	34.0	26.0	34.0
4	34.43020040190999	35.0	35.0	37.0	30.0	37.0
5	34.63177301303442	35.0	35.0	37.0	32.0	37.0
6	34.86421710514187	35.0	35.0	37.0	32.0	37.0
7	34.842333290315445	35.0	35.0	37.0	32.0	37.0
8	34.90042126804447	35.0	35.0	37.0	32.0	37.0
9	36.628684943124206	38.0	35.0	39.0	32.0	39.0
10	36.46448719603252	38.0	35.0	39.0	32.0	39.0
11	36.477793551003856	38.0	35.0	39.0	32.0	39.0
12	36.32776405302262	38.0	35.0	39.0	32.0	39.0
13	36.48751866669125	38.0	35.0	39.0	32.0	39.0
14	37.150504231116685	39.0	36.0	40.0	32.0	41.0
15	37.1691847495437	39.0	36.0	40.0	32.0	41.0
16	37.16683873822385	39.0	36.0	40.0	31.0	41.0
17	37.09174793975038	39.0	36.0	40.0	31.0	41.0
18	37.32766265371214	39.0	36.0	40.0	32.0	41.0
19	37.26188676462455	39.0	36.0	40.0	31.0	41.0
20	37.30849818403053	39.0	36.0	40.0	32.0	41.0
21	37.2824339521764	39.0	36.0	40.0	32.0	41.0
22	36.99880625357202	39.0	36.0	40.0	31.0	41.0
23	37.09557806825095	39.0	36.0	40.0	31.0	41.0
24	36.877302225253956	39.0	35.0	40.0	31.0	41.0
25	36.78564185763537	38.0	35.0	40.0	31.0	41.0
26	36.57570841245552	38.0	35.0	40.0	30.0	41.0
27	36.344831400601024	38.0	35.0	40.0	30.0	41.0
28	36.433597278811234	38.0	35.0	40.0	30.0	41.0
29	36.411999225677995	38.0	35.0	40.0	30.0	41.0
30	36.143286443834	38.0	35.0	40.0	30.0	41.0
31	36.044431334230566	38.0	35.0	40.0	30.0	41.0
32	35.786287125974816	38.0	34.0	40.0	29.0	41.0
33	35.50493630279678	38.0	34.0	40.0	28.0	41.0
34	35.425374716542834	38.0	34.0	40.0	28.0	41.0
35	35.4412990173485	38.0	34.0	40.0	28.0	41.0
36	35.2828165041205	38.0	34.0	40.0	27.0	41.0
37	35.2256319020667	38.0	34.0	40.0	27.0	41.0
38	35.082068914658656	38.0	33.0	40.0	26.0	41.0
39	34.76204347264984	38.0	33.0	40.0	25.0	41.0
40	34.31680831843071	37.0	33.0	40.0	23.0	41.0
41	34.27399476410833	37.0	33.0	40.0	23.0	41.0
42	34.09944967828764	37.0	33.0	40.0	23.0	41.0
43	32.965768514592284	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	20.0
11	15.0
12	17.0
13	16.0
14	14.0
15	14.0
16	10.0
17	19.0
18	28.0
19	38.0
20	73.0
21	181.0
22	269.0
23	494.0
24	831.0
25	1328.0
26	1973.0
27	2891.0
28	4024.0
29	5458.0
30	6802.0
31	8513.0
32	10214.0
33	12904.0
34	15994.0
35	20605.0
36	27271.0
37	35874.0
38	42597.0
39	18468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.32547335041758	24.167142936155305	19.116074556147563	25.39130915727955
2	12.047159897494515	21.186464113862208	42.28305156615844	24.483324422484838
3	14.036890912778158	31.433325344296748	36.31293670839402	18.216847034531074
4	9.353164580299035	20.332866281963828	26.982817425932414	43.33115171180472
5	13.430799579653769	37.55231282609097	29.10667207462989	19.91021551962538
6	25.962832543647796	36.569200420346235	15.346785641857636	22.121181394148337
7	20.995649047768296	35.59530613373647	15.032908685311849	28.376136133183383
8	28.250308806991022	27.319278774358878	24.76908611566896	19.661326302981138
9	19.24697184786416	11.092162755111447	14.0391954425619	55.62166995446249
10	15.418686971110414	25.930108220718644	27.073154993455134	31.578049814715804
11	29.343116830441918	19.61938386091702	21.467616747478843	29.56988256116222
12	18.35834516325289	28.233255286591323	20.32226544495861	33.086134105197175
13	36.67751332018215	15.551427886654007	22.765988827639607	25.005069965524235
14	23.854187791523017	26.102026142585867	21.759831124057445	28.283954941833667
15	29.337585958960933	25.62268394756734	17.647167271989822	27.392562821481903
16	29.725207868586494	21.806843531645804	16.845190907247286	31.62275769252042
17	18.10069873343043	34.69746132999023	17.72828672037757	29.473553216201765
18	28.462325547095368	15.519164469681607	20.853229107132982	35.165280876090044
19	30.43315941815232	24.67137405283826	20.610331667926477	24.285134861082945
20	19.80466805552995	18.409044818495236	23.789660957578214	37.9966261683966
21	30.53317601076676	17.324072196309064	27.702752530373704	24.43999926255047
22	17.52041813388396	35.68887004295643	21.194299515126932	25.59641230803267
23	25.622223041610592	23.108441953503807	25.87387769399532	25.395457310890286
24	28.774819785770912	23.473018565291937	28.265518703563725	19.486642945373426
25	23.959735255618444	28.49182352832728	22.034991980236352	25.513449235817927
26	30.220681772091222	26.78554967644402	26.26887409892886	16.724894452535903
27	19.747515716893123	29.555594476503018	24.4953079773603	26.20158182924356
28	15.903099131653178	23.13333087516823	35.72343798971258	25.240132003466016
29	23.764772035913794	18.842757323795652	26.15917848122269	31.233292159067865
30	19.473737578584466	24.796279567117125	30.992238343688356	24.737744510610053
31	21.574546929444516	19.70326874504526	33.666414704743644	25.055769620766576
32	28.043362032410908	20.72970631072436	27.17639792776682	24.050533729097914
33	16.593536254862556	31.74581958297229	33.14052100809351	18.520123154071644
34	17.1659814531443	30.188418355118824	30.927250603786803	21.718349587950076
35	24.58011467340204	19.28292251249055	39.634685938681066	16.50227687542634
36	23.792887299275456	25.348905809258675	22.12394682988883	28.73426006157704
37	21.214118471267124	23.460574104459724	29.935841890820598	25.389465533452555
38	20.576224627127083	17.251709961099536	30.044615696613263	32.12744971516012
39	30.619365424678747	18.581423646319205	25.376099260706848	25.4231116682952
40	17.502903707527516	17.90896185542302	42.63887096476835	21.949263472281118
41	24.17313471359304	21.403089913534043	23.59930679744105	30.82446857543187
42	22.894120683615714	17.161833299533562	38.16670046643683	21.777345550413894
43	25.11153924153316	17.01710882911451	33.11424936855884	24.757102560793495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	41.5
4	78.0
5	78.0
6	130.0
7	182.0
8	194.5
9	207.0
10	356.5
11	506.0
12	506.0
13	753.5
14	1001.0
15	2868.0
16	4735.0
17	4733.5
18	4732.0
19	4732.0
20	3858.0
21	2984.0
22	2309.5
23	1635.0
24	1574.5
25	1514.0
26	1514.0
27	1490.5
28	1467.0
29	1400.0
30	1333.0
31	1429.5
32	1526.0
33	1526.0
34	1786.5
35	2047.0
36	2042.5
37	2038.0
38	2289.0
39	2540.0
40	2540.0
41	3203.0
42	3866.0
43	5282.5
44	6699.0
45	9117.0
46	11535.0
47	11535.0
48	16299.5
49	21064.0
50	32419.5
51	43775.0
52	32112.0
53	20449.0
54	20449.0
55	29702.0
56	38955.0
57	31817.5
58	24680.0
59	15874.0
60	7068.0
61	7068.0
62	5785.0
63	4502.0
64	3586.5
65	2671.0
66	2188.0
67	1705.0
68	1705.0
69	1327.0
70	949.0
71	655.5
72	362.0
73	222.0
74	82.0
75	82.0
76	57.5
77	33.0
78	24.0
79	15.0
80	13.5
81	12.0
82	12.0
83	9.5
84	7.0
85	5.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	216964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.30412418650099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91024998209298	14.07468520123154
2	10.429052360146121	4.026474438155639
3	4.586586443186973	2.6562010287420956
4	2.378053148055297	1.8362493316863626
5	1.585368765370198	1.5302077764053024
6	1.1651505384046033	1.349532641359857
7	0.8834133180526705	1.1937464279788352
8	0.6780794116944823	1.0471783337327851
9	0.5276603872692978	0.9167419479729356
>10	3.889406202994055	15.201600265481833
>50	0.5682496478284745	7.621079994837854
>100	0.3438149129718502	13.128906177983444
>500	0.01910082849843612	2.7469995022215667
>1k	0.02387603562304515	8.864604266145536
>5k	0.00477520712460903	5.8203204218211315
>10k+	0.007162810686913545	17.985472244243283
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	13918	6.4148891060268065	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	13157	6.064139672941133	TruSeq Adapter, Index 8 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	11947	5.506443465275345	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	7374	3.398720525064066	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	5254	2.421599896757066	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	3905	1.7998377611032246	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3303	1.5223723751405764	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2915	1.3435408639221254	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1786	0.8231780387529728	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1598	0.7365277188842388	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1340	0.6176139820431039	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.5438690289633303	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1123	0.517597389428661	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1053	0.48533397245626	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1030	0.47473313545104256	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	938	0.43232978743017275	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	910	0.4194244206412123	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	765	0.35259305691266757	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	746	0.3438358437344444	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	685	0.3157205803727808	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	669	0.3083460850648034	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	653	0.30097158975682603	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	594	0.2737781383086595	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	478	0.22031304732582363	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	451	0.20786858649361187	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	449	0.20694677458011468	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	433	0.1995722792721373	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	420	0.1935805018344057	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	414	0.1908150660939142	No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT	408	0.18804963035342268	TruSeq Adapter, Index 8 (95% over 24bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	401	0.18482328865618258	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	394	0.1815969469589425	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	394	0.1815969469589425	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	393	0.1811360410021939	No Hit
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT	391	0.18021422908869675	TruSeq Adapter, Index 8 (95% over 21bp)
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	377	0.17376154569421656	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	372	0.17145701591047363	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	370	0.17053520399697644	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	365	0.16823067421323354	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	356	0.16408252060249628	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	354	0.1631607086889991	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.15809074316476465	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	342	0.15762983720801607	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	328	0.15117715381353589	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	319	0.14702900020279863	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	314	0.14472447041905567	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	307	0.1414981287218156	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	306	0.14103722276506703	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	279	0.12859276193285524	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	277	0.12767095001935805	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	265	0.12214007853837502	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	262	0.12075736066812928	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	262	0.12075736066812928	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	260	0.11983554875463212	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	257	0.11845283088438635	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.11707011301414061	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	249	0.11476558323039766	No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	249	0.11476558323039766	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	247	0.11384377131690049	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	245	0.11292195940340333	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	245	0.11292195940340333	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	243	0.11200014748990615	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	241	0.11107833557640898	No Hit
CTGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	239	0.11015652366291181	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	236	0.10877380579266606	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	232	0.10693018196567172	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	224	0.10324293431168304	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	221	0.10186021644143729	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	220	0.1013993104846887	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	219	0.10093840452794012	No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC	218	0.10047749857119152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	4.6090595674858504E-4	0.0
17	0.0	0.0	0.0	4.6090595674858504E-4	0.0
18	0.0	0.0	0.0	9.218119134971701E-4	0.0
19	0.0	0.0	0.0	9.218119134971701E-4	0.0
20	0.0	0.0	0.0	9.218119134971701E-4	0.0
21	0.0	0.0	0.0	0.0018436238269943402	0.0
22	0.0	0.0	0.0	0.004148153610737265	0.0
23	0.0	0.0	0.0	0.004148153610737265	0.0
24	0.0	0.0	0.0	0.007374495307977361	0.0
25	0.0	0.0	0.0	0.007374495307977361	0.0
26	0.0	0.0	0.0	0.007374495307977361	0.0
27	0.0	0.0	0.0	0.010139931048468871	0.0
28	0.0	0.0	0.0	0.02304529783742925	0.0
29	0.0	0.0	0.0	0.047473313545104256	0.0
30	0.0	0.0	0.0	0.07466676499327077	0.0
31	0.0	0.0	0.0	0.11338286536015192	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	20	0.0018386472	37.0	4
GCAACGC	20	0.0018386472	37.0	3
TAGCAAC	20	0.0018386472	37.0	1
ATTACGG	20	0.0018386472	37.0	2
ACTCAGA	40	3.808782E-8	37.0	1
TCGCCGA	20	0.0018386472	37.0	1
TCAGAGT	40	3.808782E-8	37.0	3
AGCAACG	20	0.0018386472	37.0	2
TTTACAC	20	0.0018386472	37.0	2
CTTATAC	1315	0.0	35.593155	1
TTATACA	1405	0.0	33.313168	2
CTCAGAG	45	1.0731128E-7	32.88889	2
CGTCTTC	670	0.0	31.75373	37
GATACAC	35	2.3794526E-5	31.714285	3
TGATACA	35	2.3794526E-5	31.714285	2
GAGCCGC	30	3.589944E-4	30.833334	24
CGAGCCG	30	3.589944E-4	30.833334	23
GCCGCTC	30	3.589944E-4	30.833334	26
TATACAC	1555	0.0	30.218647	3
AGGACCG	25	0.0054866415	29.6	5
>>END_MODULE