FastQCFastQC Report
Fri 10 Feb 2017
ERR1631024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363359
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT96972.6687105589788613No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG78412.157920954207822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70741.946834948356859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67491.8573917255386547No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT58351.605849861982227TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC46621.2830286300875993No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30290.8336108366656667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24010.6607790091892591No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC23030.6338084373856159No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC21900.602709716836517RNA PCR Primer, Index 6 (95% over 22bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA21750.5985815680910614No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16510.4543715719164793No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16260.44749132400738667No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14270.39272455065100903No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12990.35749768135645466No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12990.35749768135645466No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12450.3426363458728145No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11570.31841787323280835No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11070.304657377414623No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG10300.2834662138546176No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9550.2628254701273396No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9380.25814690154915665No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9030.2485145544764269No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8990.24741371481097207No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA8610.23695573798915123No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8350.22980028016369486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.22869944049824No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8210.22594734133460298No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8210.22594734133460298No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8080.2223696124218748No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7740.21301247526550876No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7690.21163642568369023No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7670.21108600585096282No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT7110.19567425053459525No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7050.19402299103641305No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7040.1937477811200493No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7030.1934725712036856No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6940.19099568195641226No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6700.1843906439636833No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6270.17255661756004392No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.1684284688145883No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6110.1681532588982246No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6070.16705241923276978No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5860.16127301098913194No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5710.15714486224367635No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5390.14833814492003777No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5370.14778772508731033No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5280.145310835840037No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5260.14476041600730957No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT5230.14393478625821846No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5040.13870579784730802No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5000.1376049581818532No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4870.13402722926912503No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4870.13402722926912503No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4820.1326511796873065No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4800.1321007598545791No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4790.13182554993821535No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4790.13182554993821535No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4670.12852303094185089No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4650.12797261110912347No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4600.12659656152730495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC4580.12604614169457753No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4520.1243948821963953No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4460.12274362269821307No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4430.12191799294912194No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4390.12081715328366711No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4360.119991523534576No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4330.11916589378548488No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4230.11641379462184781No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4140.11393690537457445No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4100.11283606570911962No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4050.11146001612730108No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4010.11035917646184626No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4000.11008396654548257No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4000.11008396654548257No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3970.10925833679639144No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3910.10760707729820922No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG3880.1067814475491181No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3790.10430455830184474No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3770.10375413846911731No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3750.10320371863638991No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3720.1023780888872988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC251.2319646E-437.01
TCAGAGT403.820969E-837.03
TTACGGG900.037.03
TCTCAAC200.001840280137.03
ACTCAGA403.820969E-837.01
GATTACG900.037.01
ATTACGG950.035.052632
CTTATAC11350.033.9030841
CGTCTTC3450.032.17391237
TTATACA12050.031.9336092
GGCCCGA352.3838473E-531.71428735
GCGAGAA303.594678E-430.83333231
AGCAACG303.594678E-430.8333322
AGAACGA303.594678E-430.83333234
AACGAGA303.594678E-430.83333236
GAACGAG303.594678E-430.83333235
CGAGAAC303.594678E-430.83333232
ACTTTTC303.594678E-430.83333237
ACGAGAG303.594678E-430.83333237
TATACAC12800.030.3515623
Produced by FastQC (version 0.11.5)