FastQCFastQC Report
Fri 10 Feb 2017
ERR1631023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434853
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT143313.2955964429358886No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG142963.287547746019919No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT123432.8384304581088324Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC78851.8132564337833705No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC47341.0886437485771054Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC42560.9787215449818674No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG19330.4445180325305333No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA12880.2961920465076704No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT11660.2681365886862917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11570.2660669237650425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.25226915762338076No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9890.2274331785683898No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6550.15062561371313984No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6070.1395874007998105No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5280.12142034204662266No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5270.12119037927759496No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5270.12119037927759496No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5120.11774093774217953No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA5000.11498138451384722No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4920.11314168236162564No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4560.10486302267662866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4400.10118361837218554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15950.034.9122281
CGTCTTC5450.033.26605637
TTATACA17800.031.4915712
TATACAC18400.030.6657623
TTATGCG250.005492631329.64
TACGAGT250.005492631329.611
GGTATCA2400.027.7500021
CCGTCTT20000.026.917537
GCCGTCT20600.026.13349336
TATAGTC551.8991339E-523.5454545
GTACTGA502.6983596E-422.21
TGCCGTC24950.021.57715235
GTATCAA6400.021.3906251
ATGCCGT25950.020.74566534
TAGGTTG450.00382204120.5555575
GAACCTA450.00382204120.5555576
TATGCCG28350.019.18518633
ATTAGGC500.0070280218.53
CCTACGA500.0070280218.59
GAGGTCG1103.8351573E-818.59
Produced by FastQC (version 0.11.5)