##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631023.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 434853 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.196505485761854 31.0 31.0 34.0 28.0 34.0 2 31.24869553619269 31.0 31.0 34.0 28.0 34.0 3 30.662435351716557 31.0 31.0 34.0 26.0 34.0 4 34.824598197551815 37.0 35.0 37.0 32.0 37.0 5 35.03432424290507 35.0 35.0 37.0 32.0 37.0 6 35.24261302095191 37.0 35.0 37.0 32.0 37.0 7 35.22654782190763 37.0 35.0 37.0 32.0 37.0 8 35.28192055706181 37.0 35.0 37.0 32.0 37.0 9 37.04521757927392 39.0 37.0 39.0 33.0 39.0 10 36.85069897183646 39.0 37.0 39.0 33.0 39.0 11 36.90759636014929 39.0 37.0 39.0 33.0 39.0 12 36.821493700169945 39.0 37.0 39.0 33.0 39.0 13 36.92578871480707 39.0 37.0 39.0 33.0 39.0 14 37.945662097306446 40.0 37.0 41.0 33.0 41.0 15 37.9744327393395 40.0 37.0 41.0 33.0 41.0 16 37.96068326537934 40.0 37.0 41.0 33.0 41.0 17 37.897896530551705 40.0 37.0 41.0 33.0 41.0 18 37.95725452049313 40.0 37.0 41.0 33.0 41.0 19 37.96270923737447 40.0 37.0 41.0 33.0 41.0 20 38.03307554506925 40.0 37.0 41.0 33.0 41.0 21 37.978542174022024 40.0 37.0 41.0 33.0 41.0 22 37.87331581017033 40.0 37.0 41.0 33.0 41.0 23 37.89789193129632 40.0 37.0 41.0 33.0 41.0 24 37.81645291627286 40.0 37.0 41.0 32.0 41.0 25 37.81399461427195 39.0 37.0 41.0 33.0 41.0 26 37.62335087949261 39.0 37.0 41.0 32.0 41.0 27 37.565009324990285 39.0 37.0 41.0 32.0 41.0 28 37.597797416598254 39.0 37.0 41.0 32.0 41.0 29 37.52154636164405 39.0 37.0 41.0 32.0 41.0 30 37.495020156236706 39.0 37.0 41.0 32.0 41.0 31 37.465561925524256 39.0 37.0 41.0 32.0 41.0 32 37.329996573554745 39.0 36.0 40.0 31.0 41.0 33 37.22325475505516 39.0 36.0 40.0 31.0 41.0 34 37.17036791743417 39.0 36.0 40.0 31.0 41.0 35 37.22459543799859 39.0 36.0 40.0 31.0 41.0 36 37.16818097150071 39.0 36.0 40.0 31.0 41.0 37 37.10488601895353 39.0 36.0 40.0 31.0 41.0 38 37.10480323235668 39.0 36.0 40.0 31.0 41.0 39 36.98407967750021 39.0 36.0 40.0 31.0 41.0 40 36.83255490936018 39.0 36.0 40.0 30.0 41.0 41 36.83260780079705 39.0 36.0 40.0 30.0 41.0 42 36.80985528442945 39.0 36.0 40.0 30.0 41.0 43 36.01730009911395 38.0 35.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 6.0 10 11.0 11 17.0 12 12.0 13 10.0 14 9.0 15 10.0 16 8.0 17 8.0 18 17.0 19 22.0 20 45.0 21 104.0 22 207.0 23 364.0 24 723.0 25 1167.0 26 1992.0 27 3036.0 28 4322.0 29 6250.0 30 8508.0 31 11031.0 32 14562.0 33 18653.0 34 24590.0 35 32099.0 36 44329.0 37 64709.0 38 107131.0 39 90896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.27147334846489 24.819421735621003 16.65873295113521 25.2503719647789 2 12.137664912050738 22.0476804805302 42.674191048469254 23.140463558949804 3 13.334851087608914 29.839049057957517 37.02239607407561 19.80370378035796 4 10.772375952333316 20.58258767905476 34.5146520778286 34.130384290783326 5 11.110881148342083 39.433325744561955 35.736674232441764 13.719118874654193 6 25.888748611599784 39.87301455894291 17.85154983408186 16.38668699537545 7 21.216595033264117 35.575240368584325 21.696067406686858 21.5120971914647 8 23.917507755494384 35.374252908454125 23.83633089802761 16.871908438023883 9 20.95880676918407 15.481323573713416 18.47520886368497 45.08466079341755 10 13.396941035246392 29.18848438437817 33.409221047112474 24.005353533262966 11 28.230459488608794 22.795519405408264 25.793773987991344 23.1802471179916 12 17.710812619436915 27.899543063977944 27.350162008770777 27.039482307814367 13 29.062004861412937 19.74667301363909 27.221152895346247 23.97016922960173 14 20.981343120548786 24.57129190784012 24.61705449887663 29.830310472734467 15 23.932685298250213 29.410398456489894 21.445867913984724 25.211048331275165 16 26.38063897455002 27.46445350497754 21.598103267081058 24.556804253391377 17 18.689994089956834 34.391851959167816 23.163459835852578 23.75469411502278 18 22.869107491497125 25.96026703276739 24.153679519285827 27.01694595644965 19 21.619719767369663 31.695538492318093 24.50460270482209 22.180139035490154 20 19.80761314743143 25.668214316102222 25.698799364382907 28.82537317208344 21 23.59900932039103 25.89380779251839 26.505738720901086 24.001444166189494 22 19.06437347793392 33.468781404290645 24.000984240651437 23.465860877123994 23 24.79596553318018 24.894849523862085 25.14504901656422 25.164135926393516 24 22.55175887023891 29.956789995699694 24.930033827523324 22.561417306538072 25 20.097136273637297 28.695674170351822 27.64612409250942 23.56106546350146 26 22.155992944742245 31.728883093827108 25.80412231259759 20.311001648833056 27 18.639172318001716 30.76004994791343 26.041903815772226 24.558873918312628 28 19.48336564310238 25.861842967623545 31.173752969394254 23.48103841987982 29 18.273071589709623 29.06453445187224 25.578068910643363 27.084325047774765 30 21.31202958241061 25.34327692346609 30.074990858979934 23.26970263514337 31 20.09529657148508 26.058001209604164 28.01820385279623 25.828498366114527 32 21.315249061176996 25.978664054289613 30.19572131271947 22.510365571813924 33 21.840713988405277 28.29484906393655 29.784317918928927 20.08011902872925 34 18.95652093925993 30.357385139345936 26.194139168868556 24.491954752525565 35 19.533037601212364 24.848167081749466 37.012737637776446 18.606057679261728 36 21.8126585305839 29.00865349899851 24.180585163262066 24.998102807155522 37 20.916493619682974 27.21816337934888 28.882633901571335 22.982709099396807 38 20.90568536953867 24.39583031507199 29.682904337787715 25.015579977601625 39 25.066631712325776 25.437561658767443 26.295552750009772 23.200253878897005 40 19.105996739127935 23.47023016973552 35.992622794369595 21.431150296766955 41 21.38147833865697 27.168721384007927 25.889208537137836 25.560591740197264 42 22.364799139019393 23.500355292478147 32.94975543459514 21.18509013390732 43 22.06101832113381 23.679956215088776 31.426941978093748 22.83208348568367 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 10.0 2 11.0 3 47.5 4 84.0 5 84.0 6 99.0 7 114.0 8 118.5 9 123.0 10 213.5 11 304.0 12 304.0 13 463.5 14 623.0 15 1498.0 16 2373.0 17 2705.0 18 3037.0 19 3037.0 20 3279.0 21 3521.0 22 4544.0 23 5567.0 24 6995.0 25 8423.0 26 8423.0 27 10446.5 28 12470.0 29 15535.0 30 18600.0 31 19789.5 32 20979.0 33 20979.0 34 22203.5 35 23428.0 36 24029.0 37 24630.0 38 26484.5 39 28339.0 40 28339.0 41 29176.0 42 30013.0 43 27664.0 44 25315.0 45 25658.5 46 26002.0 47 26002.0 48 27075.0 49 28148.0 50 41581.5 51 55015.0 52 40914.0 53 26813.0 54 26813.0 55 33023.0 56 39233.0 57 32177.5 58 25122.0 59 17166.5 60 9211.0 61 9211.0 62 7874.5 63 6538.0 64 5355.5 65 4173.0 66 3446.0 67 2719.0 68 2719.0 69 2175.5 70 1632.0 71 1349.5 72 1067.0 73 835.5 74 604.0 75 604.0 76 446.5 77 289.0 78 231.5 79 174.0 80 125.0 81 76.0 82 76.0 83 58.5 84 41.0 85 33.0 86 25.0 87 15.0 88 5.0 89 5.0 90 4.0 91 3.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 434853.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.24339874587536 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41252609579354 37.04474237014932 2 12.890824823242664 12.180127545753628 3 3.794489898792972 5.377937979776179 4 1.5632116960432645 2.954057339215522 5 0.8142890065297089 1.9234890114932865 6 0.4786459527212099 1.3567716961504515 7 0.32457702940318905 1.0733885416692601 8 0.2253480090439118 0.8516964678280521 9 0.1945319491111576 0.8271315396603677 >10 1.0899735167663003 9.797216024059233 >50 0.12216052110397368 3.980104304412545 >100 0.0806259439286139 6.743623162257972 >500 0.0034204945909108928 1.003048152814285 >1k 0.0034204945909108928 3.608433987719238 >5k 4.886420844158418E-4 1.8202611472820798 >10k+ 0.0014659262532475254 9.457970729758632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT 14331 3.2955964429358886 No Hit ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG 14296 3.287547746019919 No Hit TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT 12343 2.8384304581088324 Illumina PCR Primer Index 10 (95% over 21bp) ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC 7885 1.8132564337833705 No Hit CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC 4734 1.0886437485771054 Illumina PCR Primer Index 10 (95% over 22bp) CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC 4256 0.9787215449818674 No Hit CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG 1933 0.4445180325305333 No Hit TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA 1288 0.2961920465076704 No Hit TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT 1166 0.2681365886862917 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 1157 0.2660669237650425 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 0.25226915762338076 No Hit ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT 989 0.2274331785683898 No Hit GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC 655 0.15062561371313984 No Hit ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA 607 0.1395874007998105 No Hit GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT 528 0.12142034204662266 No Hit ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT 527 0.12119037927759496 No Hit GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC 527 0.12119037927759496 No Hit GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC 512 0.11774093774217953 No Hit ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA 500 0.11498138451384722 No Hit GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT 492 0.11314168236162564 No Hit ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA 456 0.10486302267662866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 440 0.10118361837218554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.2996276902769442E-4 0.0 5 0.0 0.0 0.0 2.2996276902769442E-4 0.0 6 0.0 0.0 0.0 2.2996276902769442E-4 0.0 7 0.0 0.0 0.0 2.2996276902769442E-4 0.0 8 0.0 0.0 0.0 2.2996276902769442E-4 0.0 9 0.0 0.0 0.0 2.2996276902769442E-4 0.0 10 0.0 0.0 0.0 2.2996276902769442E-4 0.0 11 0.0 0.0 0.0 4.5992553805538884E-4 0.0 12 0.0 0.0 0.0 4.5992553805538884E-4 0.0 13 0.0 0.0 0.0 6.898883070830833E-4 0.0 14 0.0 0.0 0.0 9.198510761107777E-4 0.0 15 0.0 0.0 0.0 9.198510761107777E-4 0.0 16 0.0 0.0 0.0 9.198510761107777E-4 0.0 17 0.0 0.0 0.0 9.198510761107777E-4 0.0 18 0.0 0.0 0.0 9.198510761107777E-4 0.0 19 0.0 0.0 0.0 0.0011498138451384722 0.0 20 0.0 0.0 0.0 0.0011498138451384722 0.0 21 0.0 0.0 0.0 0.0011498138451384722 0.0 22 0.0 0.0 0.0 0.0013797766141661666 0.0 23 0.0 0.0 0.0 0.0013797766141661666 0.0 24 0.0 0.0 0.0 0.0013797766141661666 0.0 25 0.0 0.0 0.0 0.0013797766141661666 0.0 26 0.0 0.0 0.0 0.0013797766141661666 0.0 27 0.0 0.0 0.0 0.0022996276902769444 0.0 28 0.0 0.0 0.0 0.006438957532775444 0.0 29 0.0 0.0 0.0 0.014947579986800137 0.0 30 0.0 0.0 0.0 0.027825495052351025 0.0 31 0.0 0.0 0.0 0.0657693519419206 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 1595 0.0 34.912228 1 CGTCTTC 545 0.0 33.266056 37 TTATACA 1780 0.0 31.491571 2 TATACAC 1840 0.0 30.665762 3 TTATGCG 25 0.0054926313 29.6 4 TACGAGT 25 0.0054926313 29.6 11 GGTATCA 240 0.0 27.750002 1 CCGTCTT 2000 0.0 26.9175 37 GCCGTCT 2060 0.0 26.133493 36 TATAGTC 55 1.8991339E-5 23.545454 5 GTACTGA 50 2.6983596E-4 22.2 1 TGCCGTC 2495 0.0 21.577152 35 GTATCAA 640 0.0 21.390625 1 ATGCCGT 2595 0.0 20.745665 34 TAGGTTG 45 0.003822041 20.555557 5 GAACCTA 45 0.003822041 20.555557 6 TATGCCG 2835 0.0 19.185186 33 ATTAGGC 50 0.00702802 18.5 3 CCTACGA 50 0.00702802 18.5 9 GAGGTCG 110 3.8351573E-8 18.5 9 >>END_MODULE