FastQCFastQC Report
Fri 10 Feb 2017
ERR1631022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690777
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT153862.2273468861875827No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG131671.9061144189803658No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT127141.8405360919660034TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC67670.9796214986891573No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC49960.7232435359023245RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC36850.5334572517614222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28540.4131579366423607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.39578619438690055No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA24520.35496260008656916No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG17090.2474025626215117No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA12980.18790434539656067No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT10090.14606739946466082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9720.14071111226922725No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9650.13969776063765876No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8970.12985377335956466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18400.033.5815241
CGTCTTC5300.031.7641537
TTATACA19800.031.1136362
GATTACG900.030.8333321
TATACAC21050.029.7054623
CCGTCTT18800.026.76595937
GGTATCA4650.026.6559161
GCCGTCT18950.026.35883936
TTACGGG1200.026.2083343
GTATCAA13700.024.3065681
AGCAACG551.9012337E-523.5454542
TGCCGTC22650.022.0529835
ATGCCGT23550.021.21019234
CGTGCGG555.1402376E-420.18181810
TTTAGGC656.897035E-519.9230773
TATTGAG5050.019.7821795
GCTTTAT5050.019.7821791
TATGCCG26050.019.316733
ATTGAGC4900.019.2551046
TCGCCCG609.231729E-418.510
Produced by FastQC (version 0.11.5)