##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690777
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477568013990044	31.0	31.0	34.0	28.0	34.0
2	31.487807208404448	31.0	31.0	34.0	28.0	34.0
3	30.95591196580083	31.0	31.0	34.0	27.0	34.0
4	35.0791065712958	37.0	35.0	37.0	32.0	37.0
5	35.2916440472106	37.0	35.0	37.0	33.0	37.0
6	35.47899973508093	37.0	35.0	37.0	33.0	37.0
7	35.48858459387038	37.0	35.0	37.0	33.0	37.0
8	35.512543121731035	37.0	35.0	37.0	33.0	37.0
9	37.29206820725067	39.0	37.0	39.0	34.0	39.0
10	37.129995642587986	39.0	37.0	39.0	33.0	39.0
11	37.18569234354936	39.0	37.0	39.0	33.0	39.0
12	37.11757484687533	39.0	37.0	39.0	33.0	39.0
13	37.19901791750449	39.0	37.0	39.0	33.0	39.0
14	38.293885001961556	40.0	38.0	41.0	33.0	41.0
15	38.316964809193124	40.0	38.0	41.0	34.0	41.0
16	38.31205005378002	40.0	38.0	41.0	34.0	41.0
17	38.293113407076376	40.0	38.0	41.0	33.0	41.0
18	38.35456015472432	40.0	38.0	41.0	34.0	41.0
19	38.34827013638265	40.0	38.0	41.0	34.0	41.0
20	38.30104650270637	40.0	38.0	41.0	34.0	41.0
21	38.28858517292846	40.0	38.0	41.0	34.0	41.0
22	38.21487397524816	40.0	38.0	41.0	34.0	41.0
23	38.22630168636188	40.0	38.0	41.0	34.0	41.0
24	38.1969130414012	40.0	38.0	41.0	33.0	41.0
25	38.11858675086171	40.0	37.0	41.0	33.0	41.0
26	38.02646874461657	40.0	37.0	41.0	33.0	41.0
27	37.902781939757695	40.0	37.0	41.0	33.0	41.0
28	37.92344997010613	40.0	37.0	41.0	33.0	41.0
29	37.91161691833978	40.0	37.0	41.0	33.0	41.0
30	37.74331658407851	40.0	37.0	41.0	33.0	41.0
31	37.75366435188201	40.0	37.0	41.0	33.0	41.0
32	37.695673133297724	40.0	37.0	41.0	33.0	41.0
33	37.60058166383652	40.0	37.0	41.0	32.0	41.0
34	37.58190414562152	40.0	37.0	41.0	32.0	41.0
35	37.5725205095132	40.0	37.0	41.0	32.0	41.0
36	37.52618283469195	40.0	37.0	41.0	32.0	41.0
37	37.49707503289774	40.0	37.0	41.0	32.0	41.0
38	37.46688728779331	40.0	37.0	41.0	32.0	41.0
39	37.36146397462568	39.0	36.0	41.0	32.0	41.0
40	37.2333184225879	39.0	36.0	41.0	31.0	41.0
41	37.21428767894704	39.0	36.0	41.0	31.0	41.0
42	37.15658598940034	39.0	36.0	41.0	31.0	41.0
43	36.50833626481484	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	12.0
11	17.0
12	6.0
13	12.0
14	14.0
15	14.0
16	11.0
17	17.0
18	23.0
19	39.0
20	54.0
21	135.0
22	252.0
23	466.0
24	810.0
25	1532.0
26	2381.0
27	3890.0
28	5728.0
29	8360.0
30	11157.0
31	15218.0
32	19911.0
33	26218.0
34	34239.0
35	46569.0
36	64363.0
37	95484.0
38	163818.0
39	190019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.08360295724959	22.662016830322955	15.864743614798988	24.389636597628467
2	13.173860739428209	21.960487972239957	41.14801158695208	23.71763970137975
3	14.048962255547014	28.768763291192386	35.297208795313104	21.8850656579475
4	11.02265999012706	19.265985983899288	37.97520762851108	31.736146397462566
5	12.376353005383793	37.902680604594536	35.817926769420524	13.903039620601149
6	26.900142882580052	40.76424084762521	17.37869095236234	14.956925317432399
7	23.64019068382416	33.77645752536636	22.72426557340502	19.85908621740446
8	25.171654528161763	34.20611861715141	22.818652039659685	17.803574815027137
9	23.313022871346327	14.962281604627833	19.49833303656607	42.226362487459774
10	14.380183474551123	29.038893883264787	33.12414860367384	23.456774038510257
11	30.70440967200703	23.163915416986956	25.44569376224165	20.685981148764363
12	20.23996166635542	27.164772422938228	28.136576637612425	24.458689273093924
13	29.418900745102977	20.234750143678784	26.460782568035707	23.88556654318253
14	21.608854956085683	22.880756018222957	26.680390343048483	28.829998682642877
15	24.096198918029987	29.442063068110258	22.339336717927782	24.122401295931972
16	24.79628013092503	27.2526444858471	23.696214552598015	24.254860830629855
17	20.74359742724497	31.30359001530161	24.549601390897497	23.40321116655592
18	24.434513598455073	24.960877388795517	25.906913519124114	24.697695493625293
19	25.405883519572885	26.951968580308836	26.407798754156552	21.23434914596172
20	22.458333152377683	26.06600972528037	27.02188984288707	24.453767279454873
21	25.28167556244635	26.946322764075813	25.409792161580363	22.362209511897472
22	22.475704894633143	31.12466107007037	26.442687003186265	19.956947032110218
23	23.78654761232641	27.019139317029957	25.921389971003666	23.272923099639968
24	26.61423295795894	27.141610099930947	25.75824035832114	20.48591658378898
25	22.949374400132026	29.254593016270086	26.388110779600364	21.407921803997525
26	25.991600762619484	28.945231239604098	25.246932077935426	19.81623591984099
27	22.288813900868153	29.23707650949583	28.18159840295783	20.29251118667819
28	21.027335884084156	28.40612817160965	27.78856273442804	22.77797320987815
29	26.361039814585602	25.922113793597646	26.243925318880045	21.472921072936707
30	22.064573661253924	27.439969773168478	29.842336962579818	20.653119602997783
31	23.860377516912116	28.384413493790326	25.465816030354226	22.289392958943335
32	26.313412287901883	26.32542774296191	27.125686002863443	20.235473966272764
33	20.185530207288316	30.24159750541781	28.955943813994967	20.61692847329891
34	24.024830010263802	28.25673118821269	26.47772001673478	21.240718784788722
35	23.368757211082592	25.307443646791945	30.866835462095583	20.45696368002988
36	22.63364298463904	28.034228122824008	25.562663493428417	23.76946539910854
37	21.61377694972473	26.721937759942787	28.71288418693732	22.95140110339516
38	21.947314401029566	24.576672355912255	28.97447367240079	24.50153957065739
39	24.6491993798288	25.224927871078513	26.987146358376148	23.138726390716542
40	20.69799660382439	24.315372399486375	33.00008541106609	21.986545585623148
41	21.98422935332242	25.738986677321336	26.503198572042784	25.773585397313457
42	22.303145588228908	24.73765050081285	30.841212142268777	22.11799176868946
43	22.673597991826593	24.66179389296401	30.225962937387894	22.4386451778215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	17.0
2	21.0
3	99.5
4	178.0
5	178.0
6	226.0
7	274.0
8	289.0
9	304.0
10	437.5
11	571.0
12	571.0
13	882.0
14	1193.0
15	2860.0
16	4527.0
17	5141.5
18	5756.0
19	5756.0
20	5878.0
21	6000.0
22	7287.5
23	8575.0
24	10936.5
25	13298.0
26	13298.0
27	15796.5
28	18295.0
29	21355.5
30	24416.0
31	27525.0
32	30634.0
33	30634.0
34	33459.0
35	36284.0
36	38190.0
37	40096.0
38	41717.5
39	43339.0
40	43339.0
41	43694.0
42	44049.0
43	44322.0
44	44595.0
45	44893.5
46	45192.0
47	45192.0
48	46872.0
49	48552.0
50	60011.5
51	71471.0
52	55756.5
53	40042.0
54	40042.0
55	48376.0
56	56710.0
57	47326.5
58	37943.0
59	29798.0
60	21653.0
61	21653.0
62	19142.5
63	16632.0
64	14278.0
65	11924.0
66	9784.0
67	7644.0
68	7644.0
69	6333.5
70	5023.0
71	4252.5
72	3482.0
73	2323.0
74	1164.0
75	1164.0
76	814.5
77	465.0
78	358.5
79	252.0
80	181.0
81	110.0
82	110.0
83	84.0
84	58.0
85	40.5
86	23.0
87	17.5
88	12.0
89	12.0
90	9.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	690777.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.4045809443807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92718733485952	41.600281519558465
2	11.622468474784197	11.948962429711143
3	3.052051575019855	4.706682967035982
4	1.2284843189731176	2.525988864542241
5	0.6640772311691683	1.7068305891477862
6	0.4054517198399012	1.2505245450929139
7	0.2798061348146635	1.0068321974070267
8	0.22183456817288094	0.9122650412723677
9	0.1630445633078944	0.7543113706891675
>10	1.2315148331838608	12.498303663625183
>50	0.13530117285537854	4.860310502818794
>100	0.06342240339919566	5.53104148383513
>500	0.0019731414390853404	0.7328934251606303
>1k	0.0022550187875261035	3.0047471248627895
>5k	2.8187734844076294E-4	0.9805238845515984
>10k+	8.456320453222888E-4	5.979500390688756
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	15386	2.2273468861875827	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	13167	1.9061144189803658	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	12714	1.8405360919660034	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	6767	0.9796214986891573	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	4996	0.7232435359023245	RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	3685	0.5334572517614222	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2854	0.4131579366423607	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2734	0.39578619438690055	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2452	0.35496260008656916	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	1709	0.2474025626215117	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1298	0.18790434539656067	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	1009	0.14606739946466082	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	972	0.14071111226922725	No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	965	0.13969776063765876	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	897	0.12985377335956466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.447645187955013E-4	0.0
5	0.0	0.0	0.0	1.447645187955013E-4	0.0
6	0.0	0.0	0.0	1.447645187955013E-4	0.0
7	0.0	0.0	0.0	1.447645187955013E-4	0.0
8	0.0	0.0	0.0	1.447645187955013E-4	0.0
9	0.0	0.0	0.0	1.447645187955013E-4	0.0
10	0.0	0.0	0.0	2.895290375910026E-4	0.0
11	0.0	0.0	0.0	4.342935563865039E-4	0.0
12	0.0	0.0	0.0	4.342935563865039E-4	0.0
13	0.0	0.0	0.0	4.342935563865039E-4	0.0
14	0.0	0.0	0.0	4.342935563865039E-4	0.0
15	0.0	0.0	0.0	7.238225939775064E-4	0.0
16	0.0	0.0	0.0	0.001013351631568509	0.0
17	0.0	0.0	0.0	0.001013351631568509	0.0
18	0.0	0.0	0.0	0.001013351631568509	0.0
19	0.0	0.0	0.0	0.0013028806691595116	0.0
20	0.0	0.0	0.0	0.0013028806691595116	0.0
21	0.0	0.0	0.0	0.0015924097067505144	0.0
22	0.0	0.0	0.0	0.002460996819523522	0.0
23	0.0	0.0	0.0	0.0028952903759100258	0.0
24	0.0	0.0	0.0	0.00332958393229653	0.0
25	0.0	0.0	0.0	0.003763877488683034	0.0
26	0.0	0.0	0.0	0.004198171045069538	0.0
27	0.0	0.0	0.0	0.004632464601456041	0.0
28	0.0	0.0	0.0	0.0111468679472536	0.0
29	0.0	0.0	0.0	0.025044261751621725	0.0
30	0.0	0.0	0.0	0.041113123337922365	0.0
31	0.0	0.0	0.0	0.08772729839007379	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1840	0.0	33.581524	1
CGTCTTC	530	0.0	31.76415	37
TTATACA	1980	0.0	31.113636	2
GATTACG	90	0.0	30.833332	1
TATACAC	2105	0.0	29.705462	3
CCGTCTT	1880	0.0	26.765959	37
GGTATCA	465	0.0	26.655916	1
GCCGTCT	1895	0.0	26.358839	36
TTACGGG	120	0.0	26.208334	3
GTATCAA	1370	0.0	24.306568	1
AGCAACG	55	1.9012337E-5	23.545454	2
TGCCGTC	2265	0.0	22.05298	35
ATGCCGT	2355	0.0	21.210192	34
CGTGCGG	55	5.1402376E-4	20.181818	10
TTTAGGC	65	6.897035E-5	19.923077	3
TATTGAG	505	0.0	19.782179	5
GCTTTAT	505	0.0	19.782179	1
TATGCCG	2605	0.0	19.3167	33
ATTGAGC	490	0.0	19.255104	6
TCGCCCG	60	9.231729E-4	18.5	10
>>END_MODULE