FastQCFastQC Report
Fri 10 Feb 2017
ERR1631021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913197
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT83230.9114134190103559No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG73770.8078213134734346No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT65670.7191219419249077RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC42490.4652884317403583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29400.32194586710206013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.3007018200892031No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC24120.2641270175000575RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC23140.2533954885966555No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15970.17488011896666328No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG10590.11596621539492573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9410.10304457855205393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11950.029.7238481
CGTCTTC3300.029.15151437
TATACAC12650.028.8102783
TTATACA13350.026.7453172
GGTATCA4250.026.1176471
GTGGTCG509.088417E-625.8999989
GATTACG659.3874405E-825.6153851
GTATCAA13350.024.3895111
CCGTCTT8400.022.9047637
ACGGTCA502.7015133E-422.19999911
GCCGTCT8750.021.9885736
AGACCGC705.100028E-621.14285714
CGGGGCG450.003825215220.5555554
CGTCCGG450.003825215220.5555559
TACGGTC555.141982E-420.18181810
TTACGGG951.6751619E-719.4736843
GTTCAAG2950.018.8135591
ATAAACG500.007033802618.4999983
CGCCGAG500.007033802618.4999982
CAGTTCG650.001579597917.0769239
Produced by FastQC (version 0.11.5)