##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	913197
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486793101598014	31.0	31.0	34.0	28.0	34.0
2	31.47907516121932	31.0	31.0	34.0	28.0	34.0
3	30.927558894740127	31.0	31.0	34.0	27.0	34.0
4	35.079027854887826	37.0	35.0	37.0	32.0	37.0
5	35.296368691530965	37.0	35.0	37.0	33.0	37.0
6	35.487521312487885	37.0	35.0	37.0	33.0	37.0
7	35.49697053319273	37.0	35.0	37.0	33.0	37.0
8	35.526360686686445	37.0	35.0	37.0	33.0	37.0
9	37.29457718323648	39.0	37.0	39.0	34.0	39.0
10	37.124517491844585	39.0	37.0	39.0	33.0	39.0
11	37.187596980717196	39.0	37.0	39.0	33.0	39.0
12	37.13598380196168	39.0	37.0	39.0	33.0	39.0
13	37.19895707059923	39.0	37.0	39.0	33.0	39.0
14	38.3593890474892	40.0	38.0	41.0	34.0	41.0
15	38.36277933457951	40.0	38.0	41.0	34.0	41.0
16	38.3605508997511	40.0	38.0	41.0	34.0	41.0
17	38.340933007883294	40.0	38.0	41.0	34.0	41.0
18	38.35310234264896	40.0	38.0	41.0	34.0	41.0
19	38.39646757490443	40.0	38.0	41.0	34.0	41.0
20	38.39787909947142	40.0	38.0	41.0	34.0	41.0
21	38.357094909422614	40.0	38.0	41.0	34.0	41.0
22	38.302713434231606	40.0	38.0	41.0	34.0	41.0
23	38.284634093191286	40.0	38.0	41.0	34.0	41.0
24	38.25041913190692	40.0	38.0	41.0	34.0	41.0
25	38.234756574977794	40.0	38.0	41.0	34.0	41.0
26	38.121499523104	40.0	37.0	41.0	33.0	41.0
27	38.06830508641618	40.0	37.0	41.0	33.0	41.0
28	38.03713766032959	40.0	37.0	41.0	33.0	41.0
29	37.96429576531679	40.0	37.0	41.0	33.0	41.0
30	37.888785223779756	40.0	37.0	41.0	33.0	41.0
31	37.86601357647912	40.0	37.0	41.0	33.0	41.0
32	37.79699451487467	40.0	37.0	41.0	33.0	41.0
33	37.75393918289263	40.0	37.0	41.0	33.0	41.0
34	37.68749568822499	40.0	37.0	41.0	33.0	41.0
35	37.65019267474597	40.0	37.0	41.0	33.0	41.0
36	37.63028897379208	40.0	37.0	41.0	33.0	41.0
37	37.570483696288974	40.0	37.0	41.0	32.0	41.0
38	37.52646471681357	40.0	37.0	41.0	32.0	41.0
39	37.4541681586777	40.0	37.0	41.0	32.0	41.0
40	37.39912855605088	39.0	37.0	41.0	32.0	41.0
41	37.33579610971127	39.0	36.0	41.0	31.0	41.0
42	37.28024073666471	39.0	36.0	41.0	31.0	41.0
43	36.698407901033406	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	5.0
11	8.0
12	11.0
13	5.0
14	4.0
15	6.0
16	4.0
17	8.0
18	11.0
19	41.0
20	61.0
21	153.0
22	284.0
23	586.0
24	1065.0
25	1815.0
26	3114.0
27	4821.0
28	7177.0
29	10084.0
30	14457.0
31	19316.0
32	25585.0
33	33912.0
34	44833.0
35	60522.0
36	83599.0
37	123943.0
38	217208.0
39	260552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.56714378168128	22.073988416519107	15.263300251752906	24.095567550046702
2	14.001469562427385	22.075521492076735	41.01480841483273	22.90820053066315
3	14.70547975956995	28.388179111407506	33.69152548683362	23.214815642188924
4	12.019969404192086	18.623473357884443	39.350216875438704	30.006340362484764
5	12.537491910288798	38.68880427771883	35.63546529390701	13.138238518085362
6	27.95125257748328	40.22549351344781	18.033020257403386	13.790233651665524
7	24.82180734277489	33.05869379772382	23.883127079918136	18.23637177958316
8	24.831334312311583	35.44645897873077	22.00653309198344	17.71567361697421
9	24.680216864488166	15.467418311711493	20.37742130120883	39.47494352259151
10	14.876636695039517	28.68515774799961	33.87757515629158	22.560630400669297
11	31.628553313250045	23.854327160514107	25.43503756582643	19.08208196040942
12	21.247222669369258	26.295530975244112	30.158224348086993	22.29902200729963
13	27.980600023872178	21.363845917146026	27.135218359236834	23.52033569974496
14	21.550224102794907	21.81522716347075	27.6835118818831	28.951036851851242
15	23.283585031488276	29.167857537858755	23.902181018991524	23.64637641166145
16	23.385096534482702	27.726547502893677	24.74055433822056	24.14780162440306
17	21.138921831762477	29.48038594082109	26.28961768380755	23.09107454360888
18	23.005222312381665	26.51257067204557	26.96460895075214	23.517598064820625
19	23.604216833826655	26.951030281527427	26.88773616207675	22.557016722569173
20	22.642211921414546	26.63039847918905	27.130181110976054	23.59720848842035
21	23.62885554814569	26.547940915268008	27.33999345157726	22.483210085009038
22	21.908525761692164	29.292146163423666	26.859921791245483	21.93940628363869
23	22.831218236590793	26.875690568409667	27.17245019420782	23.120641000791725
24	23.217005750128394	27.773634823592282	26.57279864038099	22.436560785898333
25	21.96481153573654	28.128870331374284	27.06393034580709	22.842387787082085
26	23.01781543303362	26.789509820991526	27.30232359501838	22.890351150956477
27	22.050992283154674	28.642560148576923	26.52603983587331	22.78040773239509
28	21.7101019823762	26.612658604879343	28.74308610299859	22.934153309745874
29	22.59085389023398	27.1105796449178	27.19621286535107	23.102353599497153
30	21.929222281720154	27.5728019255429	27.688001603159012	22.809974189577932
31	23.174955677690576	26.227090102135687	27.890148565972073	22.707805654201668
32	22.175499919513534	28.305393031295544	27.620546278623344	21.898560770567578
33	21.830995940634935	27.759070605794804	27.786227944244228	22.62370550932603
34	23.213173061234325	27.558456718539375	27.138941542733935	22.08942867749237
35	22.33351620734628	27.18876649835687	29.166433967698097	21.31128332659875
36	23.379183243046135	26.632041060143653	26.009612383746333	23.979163313063882
37	22.450467971313966	26.806592662919392	27.91007854822125	22.83286081754539
38	22.816873029587263	26.133243976929403	27.917853431406364	23.132029562076966
39	23.018034443827563	26.626456284898005	27.478189262557805	22.87732000871663
40	22.05964320951558	25.5644729450491	30.08310364576318	22.29278019967214
41	22.3937441756817	26.2348649853208	27.48957782384305	23.881813015154453
42	22.367024858820166	26.096450163546308	28.665118260353463	22.87140671728006
43	22.719960753265724	25.123275700642907	29.269259535456204	22.887504010635165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	16.0
2	15.0
3	80.0
4	145.0
5	145.0
6	194.5
7	244.0
8	256.5
9	269.0
10	419.5
11	570.0
12	570.0
13	888.0
14	1206.0
15	2956.5
16	4707.0
17	5403.0
18	6099.0
19	6099.0
20	6370.5
21	6642.0
22	8370.0
23	10098.0
24	13332.0
25	16566.0
26	16566.0
27	20440.0
28	24314.0
29	28337.0
30	32360.0
31	37225.0
32	42090.0
33	42090.0
34	47247.5
35	52405.0
36	55705.5
37	59006.0
38	61509.0
39	64012.0
40	64012.0
41	65762.5
42	67513.0
43	67641.0
44	67769.0
45	67042.5
46	66316.0
47	66316.0
48	64813.0
49	63310.0
50	68737.0
51	74164.0
52	63939.5
53	53715.0
54	53715.0
55	57549.0
56	61383.0
57	53219.5
58	45056.0
59	36084.0
60	27112.0
61	27112.0
62	24836.5
63	22561.0
64	18010.0
65	13459.0
66	11002.0
67	8545.0
68	8545.0
69	7150.0
70	5755.0
71	5005.0
72	4255.0
73	4915.0
74	5575.0
75	5575.0
76	4739.5
77	3904.0
78	2738.5
79	1573.0
80	939.5
81	306.0
82	306.0
83	200.5
84	95.0
85	64.0
86	33.0
87	28.0
88	23.0
89	23.0
90	16.0
91	9.0
92	4.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	913197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.60896038626906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20457584225754	39.07243320439517
2	13.104590065363913	13.264193589925949
3	4.073921872276172	6.185308519523395
4	1.7508695980251467	3.5443876051190997
5	0.9194286112846841	2.3265663083254475
6	0.5614798711585339	1.704954753428982
7	0.36488256427356847	1.2926429068672909
8	0.28163722897654825	1.1402693891658175
9	0.19217988077413412	0.8753421575822459
>10	1.3178125582324858	13.044026005979351
>50	0.14189009423550553	4.937391019034444
>100	0.08110830407155051	7.14236408311004
>500	0.0034606209662579527	1.1371957143539873
>1k	0.0015140216727378543	1.8955451136267203
>5k	6.488664311733661E-4	2.4373796295620593
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	8323	0.9114134190103559	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	7377	0.8078213134734346	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	6567	0.7191219419249077	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	4249	0.4652884317403583	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2940	0.32194586710206013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2746	0.3007018200892031	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	2412	0.2641270175000575	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2314	0.2533954885966555	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1597	0.17488011896666328	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1059	0.11596621539492573	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	941	0.10304457855205393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.1901079394697967E-4	0.0
10	0.0	0.0	0.0	2.1901079394697967E-4	0.0
11	0.0	0.0	0.0	2.1901079394697967E-4	0.0
12	0.0	0.0	0.0	3.2851619092046953E-4	0.0
13	0.0	0.0	0.0	3.2851619092046953E-4	0.0
14	0.0	0.0	0.0	3.2851619092046953E-4	0.0
15	0.0	0.0	0.0	3.2851619092046953E-4	0.0
16	0.0	0.0	0.0	4.3802158789395934E-4	1.0950539697348984E-4
17	0.0	0.0	0.0	5.475269848674492E-4	1.0950539697348984E-4
18	0.0	0.0	0.0	5.475269848674492E-4	1.0950539697348984E-4
19	0.0	0.0	0.0	5.475269848674492E-4	1.0950539697348984E-4
20	0.0	0.0	0.0	5.475269848674492E-4	1.0950539697348984E-4
21	0.0	0.0	0.0	7.665377788144288E-4	1.0950539697348984E-4
22	0.0	0.0	0.0	0.0010950539697348984	1.0950539697348984E-4
23	0.0	0.0	0.0	0.0013140647636818781	1.0950539697348984E-4
24	0.0	0.0	0.0	0.0016425809546023475	1.0950539697348984E-4
25	0.0	0.0	0.0	0.0016425809546023475	1.0950539697348984E-4
26	0.0	0.0	0.0	0.0017520863515758374	1.0950539697348984E-4
27	0.0	0.0	0.0	0.0026281295273637563	1.0950539697348984E-4
28	0.0	0.0	0.0	0.0070083454063033495	1.0950539697348984E-4
29	0.0	0.0	0.0	0.018615917485493273	1.0950539697348984E-4
30	0.0	0.0	0.0	0.03843639433769493	1.0950539697348984E-4
31	0.0	0.0	0.0	0.08136250995130295	1.0950539697348984E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1195	0.0	29.723848	1
CGTCTTC	330	0.0	29.151514	37
TATACAC	1265	0.0	28.810278	3
TTATACA	1335	0.0	26.745317	2
GGTATCA	425	0.0	26.117647	1
GTGGTCG	50	9.088417E-6	25.899998	9
GATTACG	65	9.3874405E-8	25.615385	1
GTATCAA	1335	0.0	24.389511	1
CCGTCTT	840	0.0	22.90476	37
ACGGTCA	50	2.7015133E-4	22.199999	11
GCCGTCT	875	0.0	21.98857	36
AGACCGC	70	5.100028E-6	21.142857	14
CGGGGCG	45	0.0038252152	20.555555	4
CGTCCGG	45	0.0038252152	20.555555	9
TACGGTC	55	5.141982E-4	20.181818	10
TTACGGG	95	1.6751619E-7	19.473684	3
GTTCAAG	295	0.0	18.813559	1
ATAAACG	50	0.0070338026	18.499998	3
CGCCGAG	50	0.0070338026	18.499998	2
CAGTTCG	65	0.0015795979	17.076923	9
>>END_MODULE