FastQCFastQC Report
Fri 10 Feb 2017
ERR1631020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831667
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT157321.89162248832766No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG122341.471021454500419No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT97721.1749895090222409RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC61500.7394786615315986No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC35890.4315429132092532RNA PCR Primer, Index 33 (95% over 23bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC30140.36240466436686797No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC29930.3598796152787113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19580.2354307673624179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.2172744620142437No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG15120.18180353434728083No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG13870.1667734802511101No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA12670.15234462831878623No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA11150.13406808253784266No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG10170.12228452012644485No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT10160.12216427969367546No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT10040.12072139450044307No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG9640.11591177718966847No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG9580.11519033459305227No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG9320.11206408334104875No Hit
TATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATCA9100.10941879382012272No Hit
GTATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATC9040.10869735122350653No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT8460.1017234061228833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20150.032.501241
TATACCG352.3875198E-531.7142875
CGTCTTC4700.031.48936337
TTATACA21900.029.7351592
CGCTCCG250.00549548129.5999985
TATACAC23150.028.6090723
TTAGCGA358.8673236E-426.42857227
CCGTCTT15400.025.70779237
GATTACG659.385258E-825.6153851
GGTATCA4950.025.4141431
GCCGTCT15850.025.09463736
TTACGGG753.738187E-722.23
GTATCAA12850.022.0272391
GTTAGCG450.003824932320.55555526
TGCCGTC19350.020.4599535
ATGCCGT20200.019.5990134
AGACACG609.2338916E-418.512
TTCGGTC609.2338916E-418.528
ACACGTA500.007033287518.49999823
TAGCGAG500.007033287518.49999828
Produced by FastQC (version 0.11.5)