##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631020.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831667
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465498811423323	31.0	31.0	34.0	28.0	34.0
2	31.481506420237906	31.0	31.0	34.0	28.0	34.0
3	30.9290809903483	31.0	31.0	34.0	27.0	34.0
4	35.060937851327516	37.0	35.0	37.0	32.0	37.0
5	35.2794688258642	37.0	35.0	37.0	33.0	37.0
6	35.46357616690334	37.0	35.0	37.0	33.0	37.0
7	35.476867544341665	37.0	35.0	37.0	33.0	37.0
8	35.504774146383106	37.0	35.0	37.0	33.0	37.0
9	37.27749087074514	39.0	37.0	39.0	34.0	39.0
10	37.116897748738374	39.0	37.0	39.0	33.0	39.0
11	37.17396385813072	39.0	37.0	39.0	33.0	39.0
12	37.10415466767348	39.0	37.0	39.0	33.0	39.0
13	37.18424802234548	39.0	37.0	39.0	33.0	39.0
14	38.32624716382879	40.0	38.0	41.0	34.0	41.0
15	38.334820306685245	40.0	38.0	41.0	34.0	41.0
16	38.32800507895588	40.0	38.0	41.0	34.0	41.0
17	38.293084852471004	40.0	38.0	41.0	33.0	41.0
18	38.31917582397762	40.0	38.0	41.0	33.0	41.0
19	38.33969966344703	40.0	38.0	41.0	34.0	41.0
20	38.32480788584854	40.0	38.0	41.0	34.0	41.0
21	38.294881244536576	40.0	38.0	41.0	34.0	41.0
22	38.216245203909736	40.0	38.0	41.0	34.0	41.0
23	38.222389490024256	40.0	38.0	41.0	34.0	41.0
24	38.16478229868445	40.0	38.0	41.0	33.0	41.0
25	38.132934215256824	40.0	37.0	41.0	33.0	41.0
26	38.017884561970114	40.0	37.0	41.0	33.0	41.0
27	37.942461345706874	40.0	37.0	41.0	33.0	41.0
28	37.93856675808948	40.0	37.0	41.0	33.0	41.0
29	37.852027313816706	40.0	37.0	41.0	33.0	41.0
30	37.78430309246369	40.0	37.0	41.0	33.0	41.0
31	37.78233595898358	40.0	37.0	41.0	33.0	41.0
32	37.69557166510154	40.0	37.0	41.0	33.0	41.0
33	37.663561257089675	40.0	37.0	41.0	33.0	41.0
34	37.58436489604613	40.0	37.0	41.0	32.0	41.0
35	37.56189797118318	40.0	37.0	41.0	32.0	41.0
36	37.54441140504553	40.0	37.0	41.0	32.0	41.0
37	37.471554119617586	40.0	37.0	41.0	32.0	41.0
38	37.4634006158715	39.0	37.0	41.0	32.0	41.0
39	37.386112470496	39.0	36.0	41.0	31.0	41.0
40	37.292891265374244	39.0	36.0	41.0	31.0	41.0
41	37.240182669265465	39.0	36.0	41.0	31.0	41.0
42	37.20266043981545	39.0	36.0	41.0	31.0	41.0
43	36.52866471797005	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	12.0
11	17.0
12	14.0
13	12.0
14	8.0
15	6.0
16	11.0
17	14.0
18	23.0
19	47.0
20	72.0
21	177.0
22	286.0
23	559.0
24	1056.0
25	1802.0
26	2897.0
27	4658.0
28	6781.0
29	9878.0
30	13642.0
31	18055.0
32	23981.0
33	31460.0
34	41742.0
35	56356.0
36	77088.0
37	114093.0
38	198037.0
39	228874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.396257155808755	21.403398235110927	14.669332797862605	25.531011811217713
2	14.154222783878645	20.893578800168818	41.32194736595296	23.63025104999958
3	14.708290698079882	27.747644189320965	33.750046593167696	23.794018519431457
4	11.977389989022049	18.83650547635051	37.26034578743656	31.925758747190887
5	12.80572633037021	38.10359194244812	35.24355300859599	13.847128718585683
6	28.69477807824526	39.18010453703225	17.36464233882071	14.760475045901785
7	25.424118066485747	32.556780538364514	22.30520148088117	19.71389991426857
8	25.12592179321772	35.17693980884176	21.703398114870495	17.993740283070025
9	24.29001030460509	15.016827648566073	19.105002362724505	41.58815968410433
10	15.039673330792253	28.504317232738586	32.80531751289879	23.65069192357037
11	31.64030795979641	22.592335634334415	25.0777053796772	20.689651026191974
12	21.539149683707542	25.5632362471999	28.577904377593438	24.31970969149912
13	28.552293165413563	19.911695426174177	26.800510300396674	24.735501108015587
14	21.878107463684383	21.585682731189287	25.982033674535604	30.554176130590726
15	24.289048381142933	27.941351526512413	22.60472039890966	25.164879693434994
16	25.199989899803647	26.58251439578581	23.016543881144738	25.200951823265804
17	21.34628402954548	30.017543079141053	24.45822667004943	24.17794622126404
18	24.114218791896274	25.474498807816108	25.39045074531032	25.020831654977293
19	24.023918226886483	28.000870540733253	24.627885920687007	23.34732531169326
20	22.8039587960085	27.104598354870397	24.958306629937223	25.133136219183882
21	26.31041029642874	25.916743119541835	24.5890482609025	23.183798323126926
22	22.47762626147244	30.86343452367354	24.828326722113538	21.830612492740485
23	25.706683083493754	25.11401799037355	24.792735554013806	24.38656337211889
24	25.44948879780008	27.88531948484189	24.430451130079707	22.23474058727832
25	22.887886618081517	27.948084990747496	25.105601160079694	24.05842723109129
26	25.528967723860628	28.284637962068953	25.117024001192785	21.06937031287763
27	22.738668241014732	30.039186357039537	24.645441023871335	22.576704378074396
28	24.143918178790308	25.117144241625557	26.52696331584637	24.21197426373777
29	23.377144939020063	28.50720300312505	25.433376579808986	22.682275478045902
30	23.913176788305897	26.898506253103704	26.643356054767114	22.544960903823284
31	26.151332203874865	25.538346477616642	25.18027046882947	23.13005084967902
32	22.848567996565933	27.182995117036025	26.22840632128003	23.74003056511801
33	24.1510123643237	26.89586096358278	26.791251787073435	22.161874885020087
34	24.533737661828592	27.571612195746614	25.383717281075235	22.510932861349556
35	22.83979044497377	25.045240462829472	28.901832103474106	23.21313698872265
36	24.044719821755585	27.490089182328987	24.09041118620794	24.37477980970749
37	22.80323735341188	26.52936812450175	27.11541999381964	23.551974528266722
38	23.595140843630922	24.48287595876715	27.41926756742783	24.502715630174094
39	24.241914131497342	25.427484798603288	26.047805191260444	24.282795878638925
40	22.22319750573246	24.106403163766267	30.91273310110898	22.757666229392292
41	22.72051193566656	25.404518875944337	26.028686962450116	25.84628222593899
42	23.304880438925675	24.881352752964826	28.51333526519629	23.30043154291321
43	23.736904313866006	23.479469547306795	28.988044493769742	23.795581645057457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	13.5
2	21.0
3	81.0
4	141.0
5	141.0
6	163.0
7	185.0
8	198.5
9	212.0
10	324.0
11	436.0
12	436.0
13	656.0
14	876.0
15	2022.0
16	3168.0
17	3714.0
18	4260.0
19	4260.0
20	4407.0
21	4554.0
22	5635.5
23	6717.0
24	9092.5
25	11468.0
26	11468.0
27	14510.5
28	17553.0
29	20943.5
30	24334.0
31	28213.0
32	32092.0
33	32092.0
34	36829.5
35	41567.0
36	44638.5
37	47710.0
38	50685.5
39	53661.0
40	53661.0
41	54825.0
42	55989.0
43	56898.5
44	57808.0
45	56858.5
46	55909.0
47	55909.0
48	55357.0
49	54805.0
50	67969.5
51	81134.0
52	65718.5
53	50303.0
54	50303.0
55	59172.5
56	68042.0
57	58941.0
58	49840.0
59	39500.0
60	29160.0
61	29160.0
62	27592.0
63	26024.0
64	20646.0
65	15268.0
66	12545.5
67	9823.0
68	9823.0
69	8355.5
70	6888.0
71	6048.5
72	5209.0
73	6429.5
74	7650.0
75	7650.0
76	6809.5
77	5969.0
78	4124.0
79	2279.0
80	1324.0
81	369.0
82	369.0
83	256.0
84	143.0
85	101.5
86	60.0
87	38.5
88	17.0
89	17.0
90	15.0
91	13.0
92	6.5
93	0.0
94	2.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	831667.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.298329258517796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4584794046167	43.12440706059352
2	10.711852666656029	11.204239954590177
3	2.837533131150805	4.451947259246332
4	1.1549609715622495	2.416101166860005
5	0.6098739926327449	1.5947695436457072
6	0.3703826742097879	1.1622237028484286
7	0.2683835251672516	0.9825206976731035
8	0.18542647151036287	0.7757995728235301
9	0.1464721946225791	0.6894225955430408
>10	1.041276823293157	10.821452133162635
>50	0.12857233662258263	4.709998583740268
>100	0.07668375623682148	7.86080758975036
>500	0.00642857836657803	2.3334952677202354
>1k	0.002755105014247727	2.6030587583053917
>5k	4.5918416904128776E-4	1.911799508729113
>10k+	4.5918416904128776E-4	3.3579566047681433
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	15732	1.89162248832766	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	12234	1.471021454500419	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	9772	1.1749895090222409	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	6150	0.7394786615315986	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	3589	0.4315429132092532	RNA PCR Primer, Index 33 (95% over 23bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3014	0.36240466436686797	No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	2993	0.3598796152787113	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1958	0.2354307673624179	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1807	0.2172744620142437	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1512	0.18180353434728083	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	1387	0.1667734802511101	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1267	0.15234462831878623	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	1115	0.13406808253784266	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1017	0.12228452012644485	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1016	0.12216427969367546	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1004	0.12072139450044307	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	964	0.11591177718966847	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	958	0.11519033459305227	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	932	0.11206408334104875	No Hit
TATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATCA	910	0.10941879382012272	No Hit
GTATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATC	904	0.10869735122350653	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	846	0.1017234061228833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.4048086553873124E-4	0.0
11	0.0	0.0	0.0	2.4048086553873124E-4	0.0
12	0.0	0.0	0.0	2.4048086553873124E-4	0.0
13	0.0	0.0	0.0	2.4048086553873124E-4	0.0
14	0.0	0.0	0.0	2.4048086553873124E-4	0.0
15	0.0	1.2024043276936562E-4	0.0	2.4048086553873124E-4	0.0
16	0.0	1.2024043276936562E-4	0.0	3.6072129830809686E-4	0.0
17	0.0	1.2024043276936562E-4	0.0	3.6072129830809686E-4	0.0
18	0.0	1.2024043276936562E-4	0.0	3.6072129830809686E-4	0.0
19	0.0	1.2024043276936562E-4	0.0	4.809617310774625E-4	0.0
20	0.0	1.2024043276936562E-4	0.0	0.0013226447604630218	0.0
21	0.0	1.2024043276936562E-4	0.0	0.0021643277898485812	1.2024043276936562E-4
22	0.0	1.2024043276936562E-4	0.0	0.0026452895209260435	1.2024043276936562E-4
23	0.0	1.2024043276936562E-4	0.0	0.003246491684772872	1.2024043276936562E-4
24	0.0	2.4048086553873124E-4	0.0	0.0034869725503116032	1.2024043276936562E-4
25	0.0	2.4048086553873124E-4	0.0	0.003727453415850334	1.2024043276936562E-4
26	0.0	2.4048086553873124E-4	0.0	0.003967934281389066	1.2024043276936562E-4
27	0.0	2.4048086553873124E-4	0.0	0.004809617310774625	1.2024043276936562E-4
28	0.0	2.4048086553873124E-4	0.0	0.01070139851647354	1.2024043276936562E-4
29	0.0	2.4048086553873124E-4	0.0	0.023446884390026297	1.2024043276936562E-4
30	0.0	2.4048086553873124E-4	0.0	0.03931862151558256	1.2024043276936562E-4
31	0.0	2.4048086553873124E-4	0.0	0.08416830293855594	1.2024043276936562E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2015	0.0	32.50124	1
TATACCG	35	2.3875198E-5	31.714287	5
CGTCTTC	470	0.0	31.489363	37
TTATACA	2190	0.0	29.735159	2
CGCTCCG	25	0.005495481	29.599998	5
TATACAC	2315	0.0	28.609072	3
TTAGCGA	35	8.8673236E-4	26.428572	27
CCGTCTT	1540	0.0	25.707792	37
GATTACG	65	9.385258E-8	25.615385	1
GGTATCA	495	0.0	25.414143	1
GCCGTCT	1585	0.0	25.094637	36
TTACGGG	75	3.738187E-7	22.2	3
GTATCAA	1285	0.0	22.027239	1
GTTAGCG	45	0.0038249323	20.555555	26
TGCCGTC	1935	0.0	20.45995	35
ATGCCGT	2020	0.0	19.59901	34
AGACACG	60	9.2338916E-4	18.5	12
TTCGGTC	60	9.2338916E-4	18.5	28
ACACGTA	50	0.0070332875	18.499998	23
TAGCGAG	50	0.0070332875	18.499998	28
>>END_MODULE