FastQCFastQC Report
Fri 10 Feb 2017
ERR1631019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075971
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT75910.7055022858422765No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG61280.5695320784667988No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT45160.41971391422259524TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC33370.31013847027475644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31060.28866949016283894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.25093613117825664No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC16340.15186282901676718No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC14770.13727135768529078Illumina Paired End PCR Primer 2 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.028.0583341
CTTATAC11200.026.593751
CGGCGTA358.869026E-426.4285712
CCGTCTT7250.024.75172237
GCCGTCT7250.024.75172236
GTATCAA18850.024.1432341
TATACAC13400.023.055973
GATTACG652.682582E-622.769231
CGTCTTC2600.022.05769237
TTATACA13950.022.0143362
TCGACCC450.003825652420.55555528
TACCGCT450.003825652420.55555533
ATGACGG555.1428E-420.18181820
AGGACCG656.901607E-519.9230775
TAACGGC951.6758713E-719.47368436
TTAACGG801.6165144E-518.535
TGCCGTC9500.018.535
GCGAGCG1054.796548E-717.619059
AACGGCC752.0671121E-417.26666637
ATGCCGT10400.017.07692334
Produced by FastQC (version 0.11.5)