##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1075971
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45591749220007	31.0	31.0	34.0	28.0	34.0
2	31.44513560309711	31.0	31.0	34.0	28.0	34.0
3	30.87637306209926	31.0	31.0	34.0	27.0	34.0
4	35.03627328245835	37.0	35.0	37.0	32.0	37.0
5	35.26745795193365	37.0	35.0	37.0	33.0	37.0
6	35.46653023176275	37.0	35.0	37.0	33.0	37.0
7	35.48676126029419	37.0	35.0	37.0	33.0	37.0
8	35.50708058116808	37.0	35.0	37.0	33.0	37.0
9	37.26974054133429	39.0	37.0	39.0	34.0	39.0
10	37.08967899692464	39.0	37.0	39.0	33.0	39.0
11	37.165145714893804	39.0	37.0	39.0	33.0	39.0
12	37.096207053907584	39.0	37.0	39.0	33.0	39.0
13	37.16489756694186	39.0	37.0	39.0	33.0	39.0
14	38.318857106743586	40.0	38.0	41.0	34.0	41.0
15	38.319610844530196	40.0	38.0	41.0	34.0	41.0
16	38.33245412748113	40.0	38.0	41.0	34.0	41.0
17	38.31199260946624	40.0	38.0	41.0	33.0	41.0
18	38.33583990646588	40.0	38.0	41.0	34.0	41.0
19	38.375962735055126	40.0	38.0	41.0	34.0	41.0
20	38.37383070733319	40.0	38.0	41.0	34.0	41.0
21	38.33528784697729	40.0	38.0	41.0	34.0	41.0
22	38.28747243187781	40.0	38.0	41.0	34.0	41.0
23	38.26378034352227	40.0	38.0	41.0	34.0	41.0
24	38.23482045519814	40.0	38.0	41.0	34.0	41.0
25	38.21344534378715	40.0	38.0	41.0	34.0	41.0
26	38.11472614038854	40.0	37.0	41.0	33.0	41.0
27	38.05646806465973	40.0	37.0	41.0	33.0	41.0
28	38.02652394906554	40.0	37.0	41.0	33.0	41.0
29	37.96298320307889	40.0	37.0	41.0	33.0	41.0
30	37.888207953560084	40.0	37.0	41.0	33.0	41.0
31	37.869319898026994	40.0	37.0	41.0	33.0	41.0
32	37.79280389527227	40.0	37.0	41.0	33.0	41.0
33	37.77711016374977	40.0	37.0	41.0	33.0	41.0
34	37.70779695735294	40.0	37.0	41.0	33.0	41.0
35	37.68385950922469	40.0	37.0	41.0	33.0	41.0
36	37.65827517656145	40.0	37.0	41.0	33.0	41.0
37	37.58993597411083	40.0	37.0	41.0	32.0	41.0
38	37.562868330094396	40.0	37.0	41.0	32.0	41.0
39	37.514051958649446	40.0	37.0	41.0	32.0	41.0
40	37.46597724288108	40.0	37.0	41.0	32.0	41.0
41	37.411923741439125	39.0	37.0	41.0	32.0	41.0
42	37.361919605639926	39.0	37.0	41.0	32.0	41.0
43	36.78162515532482	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	4.0
11	8.0
12	7.0
13	6.0
14	6.0
15	4.0
16	6.0
17	10.0
18	15.0
19	39.0
20	85.0
21	155.0
22	334.0
23	624.0
24	1168.0
25	2079.0
26	3346.0
27	5484.0
28	8193.0
29	12150.0
30	16771.0
31	22647.0
32	29895.0
33	39502.0
34	52534.0
35	71295.0
36	100300.0
37	150512.0
38	265011.0
39	293774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.08683412471154	22.521517773248537	15.09826937714864	23.293378724891284
2	13.703808002260285	23.172278806770816	41.05008406360394	22.07382912736496
3	14.851608454131199	28.834792015769946	34.06141987098165	22.252179659117207
4	11.685630932432193	19.256002252848823	40.77293904761374	28.285427767105247
5	12.169658847682697	38.82911342406069	36.4166878103592	12.584539917897416
6	27.022010816276648	41.541361244866266	18.31694348639508	13.119684452462007
7	24.1496285680562	33.770333958814874	24.335042487204582	17.744994985924343
8	24.011427817292475	36.5350924885522	22.146228848175276	17.30725084598005
9	24.592205552008373	15.807024538765448	21.277896894990665	38.32287301423551
10	13.964874517993515	29.761582793588303	34.76487749205137	21.508665196366817
11	31.817121465169603	24.209667360923294	25.83824285227018	18.134968321636922
12	20.793961919048005	26.787617881894587	31.61535022784071	20.803069971216694
13	27.77955911451145	22.576723722107754	27.405013703900945	22.238703459479854
14	20.922125224564603	22.301344552966576	28.82884390006794	27.947686322400884
15	22.379134753631835	30.172188655642206	24.602335936563346	22.84634065416261
16	22.651261046998478	29.23378046434337	25.354214937019677	22.760743551638473
17	20.834297578652215	29.869857087226325	27.076566189980955	22.2192791441405
18	22.729701822818647	27.8237982250451	27.78903892391152	21.657461028224738
19	23.1455122861118	28.31916473585255	27.59507458844151	20.940248389594142
20	22.55153716968208	27.530667648105755	27.626023377953494	22.291771804258666
21	22.98602843385184	27.361518107830047	28.030681124305396	21.62177233401272
22	21.802725166384597	29.09307035226786	27.862646855723806	21.241557625623738
23	22.077453760370865	27.727327223503234	28.09443748948624	22.10078152663966
24	22.556091195766427	28.28737949256997	27.456502080446406	21.7000272312172
25	21.794639446602186	28.390542124276585	27.604461458533734	22.210356970587497
26	22.985563737312624	28.227526578318564	27.696192555375564	21.09071712899325
27	21.8918539626068	28.623448029733144	27.60455439784158	21.880143609818482
28	21.506899349517784	27.852423531860985	28.538780320287444	22.101896798333783
29	22.618639349945305	28.09415867156271	27.67026248848714	21.616939490004842
30	21.57799792001829	28.057354705656568	28.028543520224986	22.336103854100156
31	23.150252190811834	27.158073962959968	28.142951808180705	21.548722038047494
32	22.222067323375818	27.565612827855023	28.188492069024164	22.02382777974499
33	21.86815443910663	28.017204924668043	28.80904782749721	21.305592808728115
34	22.313519602294114	27.899264943014263	27.949730987173442	21.837484467518177
35	21.567588717539785	27.451390418514997	29.22197717224721	21.75904369169801
36	23.19486305857686	27.287631358094224	27.268578799986244	22.248926783342675
37	21.889995176449922	27.665801401710645	28.43821998920045	22.005983432638985
38	21.71842921417027	27.349807755041727	28.390728002892274	22.541035027895735
39	22.348743599966912	27.269787010988217	28.184123921555504	22.197345467489367
40	21.469816565688106	26.949796974082012	29.9797113490977	21.60067511113218
41	21.853841785698684	27.05370312025138	28.06088639935463	23.031568694695302
42	21.840086768137805	26.925818632658316	29.124669716934747	22.10942488226913
43	21.997154198393822	26.58092086124998	29.22346420117271	22.19846073918349
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	22.0
2	29.0
3	108.0
4	187.0
5	187.0
6	224.5
7	262.0
8	271.5
9	281.0
10	442.5
11	604.0
12	604.0
13	989.5
14	1375.0
15	3135.0
16	4895.0
17	5981.0
18	7067.0
19	7067.0
20	7934.5
21	8802.0
22	11501.0
23	14200.0
24	18458.0
25	22716.0
26	22716.0
27	28481.5
28	34247.0
29	40730.0
30	47213.0
31	52900.0
32	58587.0
33	58587.0
34	63783.0
35	68979.0
36	72351.0
37	75723.0
38	78350.5
39	80978.0
40	80978.0
41	82408.0
42	83838.0
43	82545.5
44	81253.0
45	79915.0
46	78577.0
47	78577.0
48	75800.5
49	73024.0
50	69868.0
51	66712.0
52	70004.5
53	73297.0
54	73297.0
55	62896.5
56	52496.0
57	50347.0
58	48198.0
59	41494.5
60	34791.0
61	34791.0
62	28042.0
63	21293.0
64	17910.5
65	14528.0
66	11871.5
67	9215.0
68	9215.0
69	7387.5
70	5560.0
71	4463.0
72	3366.0
73	2612.5
74	1859.0
75	1859.0
76	1373.0
77	887.0
78	660.5
79	434.0
80	339.5
81	245.0
82	245.0
83	184.5
84	124.0
85	94.5
86	65.0
87	49.5
88	34.0
89	34.0
90	23.5
91	13.0
92	7.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1075971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.57785421750225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11678674146648	47.13608386640305
2	12.69692141220611	15.129106658149968
3	3.677380853639995	6.572713812011928
4	1.4974356667534465	3.568560154157005
5	0.7509208004245186	2.236912498829103
6	0.47367175536911743	1.6932208072997799
7	0.29033079053566696	1.2108099859370303
8	0.2255188292608567	1.0748742346404083
9	0.16989531917224698	0.9109798702092131
>10	0.9845172374119183	10.932917707577905
>50	0.08423048570968489	3.4054752233288204
>100	0.03019947406728697	2.8685897639880014
>500	7.823692795615588E-4	0.3178930412550409
>1k	0.0010953169913861824	1.6629255336971909
>5k	3.129477118246235E-4	1.2789368425155152
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	7591	0.7055022858422765	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	6128	0.5695320784667988	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	4516	0.41971391422259524	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	3337	0.31013847027475644	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3106	0.28866949016283894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2700	0.25093613117825664	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	1634	0.15186282901676718	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	1477	0.13727135768529078	Illumina Paired End PCR Primer 2 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.293930784379876E-5	0.0
4	0.0	0.0	0.0	1.8587861568759753E-4	0.0
5	0.0	0.0	0.0	1.8587861568759753E-4	0.0
6	0.0	0.0	0.0	1.8587861568759753E-4	0.0
7	0.0	0.0	0.0	1.8587861568759753E-4	0.0
8	0.0	0.0	0.0	1.8587861568759753E-4	0.0
9	0.0	0.0	0.0	1.8587861568759753E-4	0.0
10	0.0	0.0	0.0	1.8587861568759753E-4	9.293930784379876E-5
11	0.0	0.0	0.0	2.7881792353139627E-4	9.293930784379876E-5
12	0.0	0.0	0.0	2.7881792353139627E-4	9.293930784379876E-5
13	0.0	0.0	0.0	3.7175723137519506E-4	9.293930784379876E-5
14	0.0	0.0	0.0	3.7175723137519506E-4	9.293930784379876E-5
15	0.0	0.0	0.0	4.646965392189938E-4	9.293930784379876E-5
16	0.0	0.0	0.0	4.646965392189938E-4	1.8587861568759753E-4
17	0.0	0.0	0.0	4.646965392189938E-4	1.8587861568759753E-4
18	0.0	0.0	0.0	4.646965392189938E-4	1.8587861568759753E-4
19	0.0	0.0	0.0	4.646965392189938E-4	1.8587861568759753E-4
20	0.0	0.0	0.0	5.576358470627925E-4	1.8587861568759753E-4
21	0.0	0.0	0.0	8.364537705941889E-4	2.7881792353139627E-4
22	0.0	0.0	0.0	9.293930784379876E-4	2.7881792353139627E-4
23	0.0	0.0	0.0	0.001208211001969384	2.7881792353139627E-4
24	0.0	0.0	0.0	0.001579968233344579	2.7881792353139627E-4
25	0.0	0.0	0.0	0.0016729075411883777	2.7881792353139627E-4
26	0.0	0.0	0.0	0.0018587861568759752	2.7881792353139627E-4
27	0.0	0.0	0.0	0.0029740578510015605	3.7175723137519506E-4
28	0.0	0.0	0.0	0.008643355629473284	3.7175723137519506E-4
29	0.0	0.0	0.0	0.019889011878572935	3.7175723137519506E-4
30	0.0	0.0	0.0	0.03922038791008308	3.7175723137519506E-4
31	0.0	0.0	0.0	0.08931467483789061	3.7175723137519506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	600	0.0	28.058334	1
CTTATAC	1120	0.0	26.59375	1
CGGCGTA	35	8.869026E-4	26.42857	12
CCGTCTT	725	0.0	24.751722	37
GCCGTCT	725	0.0	24.751722	36
GTATCAA	1885	0.0	24.143234	1
TATACAC	1340	0.0	23.05597	3
GATTACG	65	2.682582E-6	22.76923	1
CGTCTTC	260	0.0	22.057692	37
TTATACA	1395	0.0	22.014336	2
TCGACCC	45	0.0038256524	20.555555	28
TACCGCT	45	0.0038256524	20.555555	33
ATGACGG	55	5.1428E-4	20.181818	20
AGGACCG	65	6.901607E-5	19.923077	5
TAACGGC	95	1.6758713E-7	19.473684	36
TTAACGG	80	1.6165144E-5	18.5	35
TGCCGTC	950	0.0	18.5	35
GCGAGCG	105	4.796548E-7	17.61905	9
AACGGCC	75	2.0671121E-4	17.266666	37
ATGCCGT	1040	0.0	17.076923	34
>>END_MODULE