FastQCFastQC Report
Fri 10 Feb 2017
ERR1631018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193204
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT123966.416016231548001No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG111855.789217614542142No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT93884.859112647771267TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC58603.033063497650152No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC37841.958551582782965RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC30261.5662201610732698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29721.5382704291836609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27621.429577027390737No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16410.8493612968675598No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG13380.6925322457091986No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA11370.5884971325645432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10450.540879070826691No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10390.5377735450611789No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9880.511376576054326No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT9330.48290925653713174No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9150.4735926792405954No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7640.3954369474752075No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7240.37473344237179357No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6820.35299476201320884No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6650.34419577234425786No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6490.3359143703028923No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5980.3095174012960394No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5850.3027887621374299No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5040.26086416430301645No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4880.25258276226165094No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4420.22877373139272478No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4370.226185793254798No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4290.22204509223411523No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4170.215834040703091No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4100.2122109273099936No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3930.20341193764104262No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3820.19771847373760376No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3730.19306018508933565No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3650.18891948406865283No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3560.18426119542038466No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.18219084491004328No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3490.18063808202728723No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3460.17908531914453116No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3400.17597979337901906No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3340.17287426761350697No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT3300.17080391710316556No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3130.1620049274342146No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3120.16148733980662924No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3100.16045216455145855No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3050.15786422641353182No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3000.15527628827560505No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2960.15320593776526364No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2810.1454421233514834No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2780.14388936046872736No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2730.1413014223308006No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2660.13767830893770316No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2650.1371607213101178No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2590.1340551955446057No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2560.13250243266184966No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT2420.12525620587565475TruSeq Adapter, Index 10 (95% over 23bp)
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2410.12473861824806941No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2380.12318585536531335No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2370.12266826773772799No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2370.12266826773772799No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2330.1205979172273866No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2300.11904515434463055No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2280.11800997908945984No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.11697480383428915No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2260.11697480383428915No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2240.11593962857911844No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2240.11593962857911844No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2230.1154220409515331No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2210.11438686569636239No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2200.11386927806877704No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2170.112316515186021No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2170.112316515186021No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2160.11179892755843564No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2110.10921098942050889No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA2100.10869340179292354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2050.1061054636549968No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2040.10558787602741145No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2020.10455270077224074No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2010.10403511314465538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC200.00183814937.03
TAGCAAC200.00183814937.01
ACTCAGA308.276214E-637.01
TCAGAGT308.276214E-637.03
AGCAACG200.00183814937.02
TTTACAC200.00183814937.02
CTTATAC12800.035.5546881
CGTCTTC4600.033.38043637
CGGGAGC401.5909627E-632.3756
TTATACA14350.031.9721242
TCTACAC352.3781127E-531.7142853
GCAACGC303.588501E-430.8333343
GGGAATG303.588501E-430.83333417
TATACAC15450.029.6957913
CTATACA250.00548517329.5999981
GGATCCT250.00548517329.59999818
GAATGGT250.00548517329.59999819
ACCTGAG250.00548517329.59999832
GATCCTC250.00548517329.59999819
ACCTTAG250.00548517329.5999981
Produced by FastQC (version 0.11.5)