FastQCFastQC Report
Fri 10 Feb 2017
ERR1631017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865751
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT78820.910423435837787No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG68600.7923756368748058No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA55610.6423324951400575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47570.5494651464451096No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT45760.5285584423234856TruSeq Adapter, Index 7 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.512272004306088No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC37720.43569109362853753No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC18710.2161129470251839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16170.18677425726334707No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA14660.1693327527198929No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC14110.16297988682658177RNA PCR Primer, Index 7 (95% over 22bp)
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC13310.1537393546181292No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC10730.12393863824586976No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA10280.11874083887861522No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG9760.11273449294312106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9560.11042435989100793No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG9340.10788321353368346No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT8910.10291642747164023No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG8730.1008373077247384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11300.029.4690251
GGTATCA6200.028.943551
GATTACG652.9885996E-928.4615381
TATACAC12750.026.9882353
TTATACA12500.026.0480022
CCGTCTT7650.025.63398737
GTATCAA20900.025.2272721
GCCGTCT7900.024.82278436
GCTTTAT10000.024.6050011
CGTCTTC2700.023.29629537
TTATTGA11050.022.769234
ATTGAGC11200.021.8035726
TATTGAG11850.021.5443045
TTTGGCG705.0995222E-621.14285922
GGACCGT705.0995222E-621.1428596
AGCCTGT11900.020.98739610
CTTTATT12500.020.8680022
TTTATTG13100.020.618323
CTCGAAA450.003825056620.55555528
GCCTGTG12400.020.43951611
Produced by FastQC (version 0.11.5)