##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631017.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865751
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30999444413001	31.0	31.0	34.0	28.0	34.0
2	31.29080301379958	31.0	31.0	34.0	28.0	34.0
3	30.704350327057085	31.0	31.0	34.0	26.0	34.0
4	34.890411908273855	37.0	35.0	37.0	32.0	37.0
5	35.11924329281745	37.0	35.0	37.0	32.0	37.0
6	35.337695249557896	37.0	35.0	37.0	33.0	37.0
7	35.3567480719052	37.0	35.0	37.0	33.0	37.0
8	35.3871667488689	37.0	35.0	37.0	33.0	37.0
9	37.12466748522381	39.0	37.0	39.0	33.0	39.0
10	36.94278839989789	39.0	37.0	39.0	33.0	39.0
11	37.00890787304895	39.0	37.0	39.0	33.0	39.0
12	36.93648866706478	39.0	37.0	39.0	33.0	39.0
13	37.017039541392386	39.0	37.0	39.0	33.0	39.0
14	38.102115966369084	40.0	37.0	41.0	33.0	41.0
15	38.10618526574038	40.0	37.0	41.0	33.0	41.0
16	38.10996348834711	40.0	37.0	41.0	33.0	41.0
17	38.10473912244976	40.0	37.0	41.0	33.0	41.0
18	38.13918320625677	40.0	37.0	41.0	33.0	41.0
19	38.17851784173509	40.0	38.0	41.0	33.0	41.0
20	38.17435613704171	40.0	38.0	41.0	33.0	41.0
21	38.12646938900446	40.0	37.0	41.0	33.0	41.0
22	38.0572554926301	40.0	37.0	41.0	33.0	41.0
23	38.061629729564274	40.0	37.0	41.0	33.0	41.0
24	38.01882873944125	40.0	37.0	41.0	33.0	41.0
25	37.99492232755146	40.0	37.0	41.0	33.0	41.0
26	37.88297905517868	40.0	37.0	41.0	33.0	41.0
27	37.793229231037564	40.0	37.0	41.0	33.0	41.0
28	37.77352495116956	40.0	37.0	41.0	33.0	41.0
29	37.70164862645264	39.0	37.0	41.0	33.0	41.0
30	37.60923290876938	39.0	37.0	41.0	32.0	41.0
31	37.578385702124514	39.0	37.0	41.0	32.0	41.0
32	37.47953741895765	39.0	36.0	41.0	32.0	41.0
33	37.45577539038361	39.0	36.0	41.0	32.0	41.0
34	37.390511821528364	39.0	36.0	41.0	32.0	41.0
35	37.34202097369798	39.0	36.0	41.0	32.0	41.0
36	37.315057100713716	39.0	36.0	41.0	32.0	41.0
37	37.22925644902518	39.0	36.0	41.0	31.0	41.0
38	37.17681296354264	39.0	36.0	41.0	31.0	41.0
39	37.09615813322768	39.0	36.0	40.0	31.0	41.0
40	37.00774472105721	39.0	36.0	40.0	31.0	41.0
41	36.94473122179472	39.0	36.0	40.0	31.0	41.0
42	36.873016606391445	39.0	36.0	40.0	31.0	41.0
43	36.22676959079458	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	12.0
12	5.0
13	5.0
14	5.0
15	4.0
16	5.0
17	6.0
18	23.0
19	39.0
20	95.0
21	189.0
22	342.0
23	659.0
24	1235.0
25	2168.0
26	3473.0
27	5438.0
28	7980.0
29	11476.0
30	15545.0
31	20743.0
32	27122.0
33	34814.0
34	46193.0
35	62028.0
36	86496.0
37	127180.0
38	213273.0
39	199193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03772447274101	21.943376328759655	15.450285359185262	22.568613839314075
2	14.279163408416506	22.915364810436255	39.684620635725516	23.12085114542172
3	15.445896106386247	28.550934391066253	33.93204281600599	22.07112668654151
4	11.844745198099686	18.510518613319533	40.04107416566657	29.60366202291421
5	12.723750824428734	38.28548855271319	35.898890096575116	13.091870526282962
6	27.517496370203443	41.13827185876771	17.37023693879649	13.97399483223236
7	24.8559920808639	32.81151277907851	23.84761900361651	18.484876136441077
8	25.545104770309244	34.11673795352244	22.108781855290953	18.229375420877368
9	25.202050012070444	15.3734734352025	20.332174031563348	39.09230252116371
10	14.902668319181844	29.599503783420406	33.26175771093536	22.23607018646239
11	32.43715571798358	24.13707867504629	24.9152469936506	18.510518613319533
12	22.262521209909085	26.481979229593726	29.424511204722837	21.830988355774352
13	29.182755780819196	21.551115736510845	26.345103846256023	22.921024636413932
14	21.781667015111736	21.725530781945388	28.57195660184048	27.920845601102396
15	23.616259178447383	29.65344539018725	23.941525912184915	22.78876951918046
16	22.959026325121197	27.91541678842993	25.316286091497442	23.809270794951434
17	22.207078016658368	28.902883161555692	25.936903335947637	22.95313548583831
18	23.478806261846653	26.1199813803276	28.122751229857084	22.278461127968665
19	25.09370477192634	26.43323542219414	28.026418681583966	20.446641124295553
20	24.56306720985595	26.47631940361605	26.543544275432545	22.417069111095454
21	24.45298936992276	25.800605485872957	27.514262183930484	22.232142960273794
22	21.945686461811768	28.93152881140189	27.675393964315376	21.447390762470963
23	23.771269106244176	25.719635322396396	27.612904865255715	22.896190706103717
24	22.056110821702777	27.021164283956935	28.190091608326178	22.73263328601411
25	22.26367627643514	28.231096470001187	27.639009368744595	21.866217884819076
26	23.74424054953445	26.40597585217921	27.94845169107515	21.901331907211198
27	22.32916854846255	28.015445549586428	27.58218009566261	22.073205806288414
28	23.302081083359997	26.21677595521114	28.02202942878495	22.45911353264391
29	22.125068293308352	26.975192636219887	28.212961925542103	22.686777144929664
30	23.669969771909013	27.125582297912448	27.553649952469012	21.650797977709527
31	23.061249712677203	26.102539875784146	27.270427640279944	23.56578277125871
32	21.778086308880958	27.022203843830383	28.77998408318327	22.419725764105383
33	21.90387305356852	26.889255686681274	28.66355337735677	22.54331788239344
34	22.89665273271414	26.519980918300988	27.56520061772958	23.01816573125529
35	21.566478121307398	26.215274368727265	30.026647384756128	22.191600125209213
36	22.27522694169571	26.681112698685883	27.48931274696766	23.55434761265075
37	22.43208497593419	26.532917663392823	28.996559056818878	22.03843830385411
38	22.573349612070906	25.86009141196487	28.675912589185575	22.890646386778645
39	23.615681645184356	26.229597193650367	27.583104148883454	22.571617012281823
40	21.998704015357763	25.698151085011737	30.179809206111223	22.12333569351927
41	22.137311998484552	26.19251955816395	27.326101846835872	24.344066596515628
42	22.1668817015516	25.883885782401638	28.9888201110943	22.960412404952464
43	22.77698784061468	26.083943304714634	28.815675638838417	22.323393215832265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36.0
1	38.5
2	41.0
3	167.0
4	293.0
5	293.0
6	383.5
7	474.0
8	463.5
9	453.0
10	652.0
11	851.0
12	851.0
13	1375.0
14	1899.0
15	4553.5
16	7208.0
17	7974.5
18	8741.0
19	8741.0
20	8601.5
21	8462.0
22	9913.0
23	11364.0
24	14503.5
25	17643.0
26	17643.0
27	21263.0
28	24883.0
29	29012.5
30	33142.0
31	36681.5
32	40221.0
33	40221.0
34	43434.0
35	46647.0
36	49157.0
37	51667.0
38	53375.0
39	55083.0
40	55083.0
41	55751.5
42	56420.0
43	56534.5
44	56649.0
45	57550.5
46	58452.0
47	58452.0
48	59138.0
49	59824.0
50	58296.0
51	56768.0
52	61574.5
53	66381.0
54	66381.0
55	57362.5
56	48344.0
57	46793.5
58	45243.0
59	40496.5
60	35750.0
61	35750.0
62	30839.5
63	25929.0
64	22547.5
65	19166.0
66	15544.0
67	11922.0
68	11922.0
69	9820.5
70	7719.0
71	6662.0
72	5605.0
73	3536.0
74	1467.0
75	1467.0
76	998.5
77	530.0
78	396.0
79	262.0
80	184.0
81	106.0
82	106.0
83	87.0
84	68.0
85	45.0
86	22.0
87	16.5
88	11.0
89	11.0
90	7.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	865751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.618500592558114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06716347804809	40.813284248435146
2	12.520397804408448	12.925683229718418
3	3.59843624074766	5.572376496759471
4	1.4416043208509615	2.976538139603188
5	0.7940171242605465	2.0492986699572153
6	0.4537668298673166	1.405365802583358
7	0.29817530597547354	1.0773953545726827
8	0.21647288678692672	0.8939204667906995
9	0.17471529895213225	0.8116687586240647
>10	1.2208141751428643	12.411845318808243
>50	0.1265607840913719	4.570922848361697
>100	0.08049687102778177	7.512240192977907
>500	0.004248445968736419	1.4808413736749693
>1k	0.0024596266134789793	3.1552424498949834
>5k	6.708072582215398E-4	2.34337664923795
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	7882	0.910423435837787	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	6860	0.7923756368748058	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	5561	0.6423324951400575	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4757	0.5494651464451096	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	4576	0.5285584423234856	TruSeq Adapter, Index 7 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4435	0.512272004306088	No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	3772	0.43569109362853753	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	1871	0.2161129470251839	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1617	0.18677425726334707	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1466	0.1693327527198929	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	1411	0.16297988682658177	RNA PCR Primer, Index 7 (95% over 22bp)
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1331	0.1537393546181292	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	1073	0.12393863824586976	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1028	0.11874083887861522	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	976	0.11273449294312106	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	956	0.11042435989100793	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	934	0.10788321353368346	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	891	0.10291642747164023	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	873	0.1008373077247384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.465199578169705E-4	0.0
17	0.0	0.0	0.0	3.465199578169705E-4	0.0
18	0.0	0.0	0.0	3.465199578169705E-4	0.0
19	0.0	0.0	0.0	4.620266104226273E-4	0.0
20	0.0	0.0	0.0	4.620266104226273E-4	0.0
21	0.0	0.0	0.0	9.240532208452546E-4	0.0
22	0.0	0.0	0.0	0.0010395598734509114	0.0
23	0.0	0.0	0.0	0.0015015864838735387	0.0
24	0.0	0.0	0.0	0.001963613094296166	0.0
25	0.0	0.0	0.0	0.00254114635732445	0.0
26	0.0	0.0	0.0	0.002772159662535764	0.0
27	0.0	0.0	0.0	0.0028876663151414204	0.0
28	0.0	0.0	0.0	0.010626612039720427	0.0
29	0.0	0.0	0.0	0.02656653009930107	0.0
30	0.0	0.0	0.0	0.048628300746981525	0.0
31	0.0	0.0	0.0	0.10153034764037235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1130	0.0	29.469025	1
GGTATCA	620	0.0	28.94355	1
GATTACG	65	2.9885996E-9	28.461538	1
TATACAC	1275	0.0	26.988235	3
TTATACA	1250	0.0	26.048002	2
CCGTCTT	765	0.0	25.633987	37
GTATCAA	2090	0.0	25.227272	1
GCCGTCT	790	0.0	24.822784	36
GCTTTAT	1000	0.0	24.605001	1
CGTCTTC	270	0.0	23.296295	37
TTATTGA	1105	0.0	22.76923	4
ATTGAGC	1120	0.0	21.803572	6
TATTGAG	1185	0.0	21.544304	5
TTTGGCG	70	5.0995222E-6	21.142859	22
GGACCGT	70	5.0995222E-6	21.142859	6
AGCCTGT	1190	0.0	20.987396	10
CTTTATT	1250	0.0	20.868002	2
TTTATTG	1310	0.0	20.61832	3
CTCGAAA	45	0.0038250566	20.555555	28
GCCTGTG	1240	0.0	20.439516	11
>>END_MODULE