FastQCFastQC Report
Fri 10 Feb 2017
ERR1631016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174170
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT159459.154848711029453No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG139818.027214790147557No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT135417.774588046161796RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC71954.131021415858069No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC52433.0102773152666935RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC41722.395360854337716RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24351.3980593672848367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21721.247057472584257No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG16300.9358672561290693No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA13210.7584543836481599No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT11640.6683125681805133No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10150.5827639662398806No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9570.5494631681690303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9250.531090314060975No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6680.3835333295056554No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT5830.3347304357811334Illumina PCR Primer Index 11 (95% over 24bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5670.3255440087271057No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4520.2595165642762818No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4460.25607165413102145No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4360.2503301372222541No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4230.24286616524085664No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT4180.239995406786473Illumina PCR Primer Index 11 (95% over 21bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4130.23712464833208932No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4110.2359763449503359No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3960.2273640695871849No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2960.16994890049951197No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2950.16937474880863523No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2910.16707814204512833No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2790.16018832175460757No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2760.15846586668197737No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2760.15846586668197737No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2720.15616925991847047No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA2670.1532985014640868No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2650.15215019808233335No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2630.1510018947005799No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2610.14985359131882645No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2580.14813113624619625No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.14411207441005913No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2470.14181546764655223No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2390.1372222541195384No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2310.13262904059252453No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2200.1263133719928805RNA PCR Primer, Index 39 (95% over 23bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2180.12516506861112706No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1990.1142561864844692No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1950.1119595797209623No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1950.1119595797209623No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1920.11023712464833209No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1910.10966297295745536No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT1870.10736636619394845No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1810.10392145604868805No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1770.10162484928518115No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA1770.10162484928518115No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.10105069759430442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACAT200.001837651637.02
TCGCCGA200.001837651637.01
GGGGTGG200.001837651637.019
CTTATAC17100.036.242691
TTATACA18600.033.3198932
GATACAC451.07103915E-732.888893
TGATACA451.07103915E-732.888892
TAGCTAC401.5897695E-632.3755
GTATTAG401.5897695E-632.3751
CCGTTTC401.5897695E-632.37511
GCTACCG401.5897695E-632.3757
TATTAGC401.5897695E-632.3752
CGTCTTC7100.032.04929437
TATACAC19800.031.3005033
CGGAGAT303.5870605E-430.8333321
CAGCAGT250.00548370429.621
GATATTA250.00548370429.69
ATGATAT250.00548370429.67
GGATCAA250.00548370429.62
AGGCAGC250.00548370429.618
Produced by FastQC (version 0.11.5)