##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174170
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11470976632026	31.0	31.0	34.0	28.0	34.0
2	31.230435781133377	31.0	31.0	34.0	28.0	34.0
3	30.694023080897974	31.0	31.0	34.0	26.0	34.0
4	34.774748808635245	37.0	35.0	37.0	32.0	37.0
5	34.990331285525635	35.0	35.0	37.0	32.0	37.0
6	35.183918011138545	36.0	35.0	37.0	32.0	37.0
7	35.14804501349256	37.0	35.0	37.0	32.0	37.0
8	35.18776482746742	37.0	35.0	37.0	32.0	37.0
9	37.00773956479302	39.0	37.0	39.0	33.0	39.0
10	36.82788080610897	39.0	37.0	39.0	32.0	39.0
11	36.85987253832462	39.0	37.0	39.0	32.0	39.0
12	36.730562094505366	39.0	35.0	39.0	32.0	39.0
13	36.85731182178331	39.0	37.0	39.0	32.0	39.0
14	37.598937819371876	39.0	37.0	41.0	32.0	41.0
15	37.64106907044841	39.0	37.0	41.0	32.0	41.0
16	37.61261985416547	39.0	37.0	41.0	32.0	41.0
17	37.584612734684505	39.0	37.0	41.0	32.0	41.0
18	37.759832347706265	39.0	37.0	41.0	32.0	41.0
19	37.68879255899409	39.0	37.0	41.0	32.0	41.0
20	37.80314060974909	39.0	37.0	41.0	33.0	41.0
21	37.75239708330941	39.0	37.0	41.0	32.0	41.0
22	37.52894298673709	39.0	36.0	41.0	32.0	41.0
23	37.66774415800654	39.0	37.0	41.0	32.0	41.0
24	37.43430556352988	39.0	36.0	41.0	32.0	41.0
25	37.34544410633289	39.0	36.0	41.0	31.0	41.0
26	37.227834873973705	39.0	36.0	41.0	31.0	41.0
27	36.9702704254464	39.0	35.0	40.0	31.0	41.0
28	37.167652293736005	39.0	36.0	40.0	31.0	41.0
29	36.99982201297583	39.0	35.0	40.0	31.0	41.0
30	36.90358844806798	39.0	35.0	40.0	31.0	41.0
31	36.87605787449044	39.0	35.0	40.0	31.0	41.0
32	36.46099213412184	38.0	35.0	40.0	30.0	41.0
33	36.35070333582132	38.0	35.0	40.0	30.0	41.0
34	36.16385715105931	38.0	35.0	40.0	30.0	41.0
35	36.13459838089223	38.0	35.0	40.0	30.0	41.0
36	36.14165470517311	38.0	35.0	40.0	30.0	41.0
37	35.97820520181432	38.0	35.0	40.0	30.0	41.0
38	35.918292472871336	38.0	35.0	40.0	30.0	41.0
39	35.63106160647643	38.0	35.0	40.0	28.0	41.0
40	35.14916460928978	38.0	33.0	40.0	26.0	41.0
41	35.17770568984326	38.0	34.0	40.0	26.0	41.0
42	35.02582534305564	38.0	34.0	40.0	26.0	41.0
43	33.973416776712405	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	14.0
11	12.0
12	15.0
13	14.0
14	12.0
15	6.0
16	18.0
17	15.0
18	15.0
19	30.0
20	40.0
21	84.0
22	171.0
23	284.0
24	503.0
25	770.0
26	1175.0
27	1827.0
28	2584.0
29	3563.0
30	4409.0
31	5788.0
32	7071.0
33	8778.0
34	11083.0
35	14536.0
36	19143.0
37	26209.0
38	36256.0
39	29735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.106275477981285	24.538094964689673	19.80708503186542	28.548544525463626
2	9.72785209852443	19.235804099443072	45.420566113567205	25.61577768846529
3	11.343514956651548	31.496813458115636	40.03674570821611	17.122925877016705
4	7.464546133088362	22.260435206981686	23.814089682494117	46.46092897743584
5	12.360911752885112	36.52867887695929	30.74467474306712	20.36573462708848
6	25.102486076821496	36.91221220646495	14.457713727966928	23.527587988746625
7	19.03427685594534	36.37250961704082	13.241660446690014	31.351553080323825
8	28.155250617213067	27.841189642303497	25.105356835275877	18.898202905207555
9	16.876040649939714	10.794625940173393	12.420049377045416	59.909284032841484
10	14.279152552104266	27.18665671470403	26.08371131652983	32.45047941666188
11	26.085433771602457	18.144915886777284	23.309984497904345	32.45966584371591
12	16.037205029568813	28.365390136073948	17.45478555434346	38.142619280013776
13	36.20256071654131	13.64127002354022	24.061549061261985	26.094620198656486
14	23.66021702933915	26.826663604524313	18.480220474249297	31.032898891887235
15	29.18700120571855	25.418843658494573	15.059998851696617	30.33415628409026
16	33.74576563127978	20.383533329505653	14.606993167594878	31.263707871619683
17	15.891944651776999	39.23465579606132	15.293678589883447	29.579720962278234
18	27.76138255727163	13.501177010966298	18.105873571797666	40.6315668599644
19	21.737383016592986	24.817706838146638	21.20112533731412	32.243784807946255
20	16.80140093012574	17.46799104323362	26.99259344318769	38.73801458345295
21	28.233335247172302	14.917035080668311	21.856806568295344	34.99282310386404
22	14.539243268071425	36.97938795429753	29.30872136418442	19.172647413446633
23	23.407016133662513	14.678187977263594	22.62444737899753	39.29034851007636
24	23.871504851581786	25.945914910719413	30.49147384739048	19.69110639030832
25	14.658092668082908	33.912843773324916	23.456967330768787	27.97209622782339
26	23.736579204225755	18.543377160245736	21.92053740598266	35.79950622954585
27	14.30269277143021	34.27226273181375	35.576735373485675	15.848309123270369
28	12.03996095768502	15.613481081701785	29.131882643394384	43.214675317218806
29	13.918585290233679	17.442728368835045	34.67761382557272	33.96107251535856
30	13.17620715393007	19.116380547740714	40.24344031693173	27.463971981397485
31	19.02910949072745	13.706149164609291	28.239076764081073	39.025664580582195
32	20.523626342079577	27.12751909054372	35.82878796578056	16.52006660159614
33	13.706723316300168	17.836022277085604	33.33122811046679	35.126026296147444
34	14.877992765688694	31.884365849457424	32.65545157030487	20.582189814549004
35	16.55164494459436	26.064764310730894	41.88034678762129	15.503243957053453
36	22.77372681862548	17.91066199689958	19.92421197680427	39.391399207670666
37	19.205374059826603	25.01923408164437	30.1406671642648	25.63472469426423
38	19.358672561290692	15.018659929953493	32.59975885628983	33.02290865246598
39	31.33605098467015	17.339381064477237	24.007578802319575	27.316989148533043
40	15.29310443819257	15.211000746397199	48.424527760234255	21.071367055175976
41	22.827697077567894	21.112705976919102	22.348280415685824	33.71131652982718
42	24.040879600390422	14.850433484526611	40.96112993052765	20.14755698455532
43	23.796865131767813	14.997416317391055	36.891542745593384	24.314175805247746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	38.5
4	73.0
5	73.0
6	104.5
7	136.0
8	161.5
9	187.0
10	274.0
11	361.0
12	361.0
13	564.5
14	768.0
15	2132.0
16	3496.0
17	3480.0
18	3464.0
19	3464.0
20	2844.5
21	2225.0
22	1707.0
23	1189.0
24	1196.0
25	1203.0
26	1203.0
27	1238.0
28	1273.0
29	1230.0
30	1187.0
31	1225.0
32	1263.0
33	1263.0
34	1417.0
35	1571.0
36	1734.5
37	1898.0
38	1996.0
39	2094.0
40	2094.0
41	2334.0
42	2574.0
43	3335.0
44	4096.0
45	5699.5
46	7303.0
47	7303.0
48	10893.0
49	14483.0
50	28454.5
51	42426.0
52	28045.0
53	13664.0
54	13664.0
55	23954.5
56	34245.0
57	27338.0
58	20431.0
59	12771.5
60	5112.0
61	5112.0
62	4174.5
63	3237.0
64	2538.0
65	1839.0
66	1529.0
67	1219.0
68	1219.0
69	969.5
70	720.0
71	502.5
72	285.0
73	176.0
74	67.0
75	67.0
76	53.0
77	39.0
78	29.5
79	20.0
80	14.5
81	9.0
82	9.0
83	7.5
84	6.0
85	4.0
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	174170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.193891026009073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19772659288064	14.049491875753576
2	10.840562369129525	4.161451455474537
3	4.313490876458271	2.4837802147327324
4	2.485791205504038	1.9084802204742493
5	1.5495064313490876	1.4870528793707296
6	1.136703559676937	1.3090658551989436
7	0.7149267125336524	0.9605557788367686
8	0.5773257553096022	0.8864902107136705
9	0.5294645527968891	0.9146236435666304
>10	3.942566556984745	15.074926795659414
>50	0.41280287167215074	5.418843658494574
>100	0.24827998803469936	9.137050008612276
>500	0.014956625785222853	2.1243612562439
>1k	0.020939276099311996	7.985875868404432
>5k	0.005982650314089142	7.141298731124763
>10k+	0.008973975471133712	24.956651547338808
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	15945	9.154848711029453	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	13981	8.027214790147557	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	13541	7.774588046161796	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	7195	4.131021415858069	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	5243	3.0102773152666935	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	4172	2.395360854337716	RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2435	1.3980593672848367	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2172	1.247057472584257	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1630	0.9358672561290693	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1321	0.7584543836481599	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1164	0.6683125681805133	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1015	0.5827639662398806	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	957	0.5494631681690303	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	925	0.531090314060975	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	668	0.3835333295056554	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	583	0.3347304357811334	Illumina PCR Primer Index 11 (95% over 24bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	567	0.3255440087271057	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	452	0.2595165642762818	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	446	0.25607165413102145	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	436	0.2503301372222541	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	423	0.24286616524085664	No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	418	0.239995406786473	Illumina PCR Primer Index 11 (95% over 21bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	413	0.23712464833208932	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	411	0.2359763449503359	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	396	0.2273640695871849	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	296	0.16994890049951197	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	295	0.16937474880863523	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	291	0.16707814204512833	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	279	0.16018832175460757	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	276	0.15846586668197737	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	276	0.15846586668197737	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	272	0.15616925991847047	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	267	0.1532985014640868	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	265	0.15215019808233335	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	263	0.1510018947005799	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	261	0.14985359131882645	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	258	0.14813113624619625	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	251	0.14411207441005913	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	247	0.14181546764655223	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	239	0.1372222541195384	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	231	0.13262904059252453	No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	220	0.1263133719928805	RNA PCR Primer, Index 39 (95% over 23bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	218	0.12516506861112706	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	199	0.1142561864844692	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	195	0.1119595797209623	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	195	0.1119595797209623	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	192	0.11023712464833209	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	191	0.10966297295745536	No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	187	0.10736636619394845	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	181	0.10392145604868805	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	177	0.10162484928518115	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	177	0.10162484928518115	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.10105069759430442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.741516908767296E-4	0.0
10	0.0	0.0	0.0	0.0011483033817534592	0.0
11	0.0	0.0	0.0	0.0011483033817534592	0.0
12	0.0	0.0	0.0	0.0011483033817534592	0.0
13	0.0	0.0	0.0	0.0011483033817534592	0.0
14	0.0	0.0	0.0	0.0011483033817534592	0.0
15	0.0	0.0	0.0	0.0017224550726301889	0.0
16	0.0	0.0	0.0	0.0022966067635069184	0.0
17	0.0	0.0	0.0	0.002870758454383648	0.0
18	0.0	0.0	0.0	0.002870758454383648	0.0
19	0.0	0.0	0.0	0.0034449101452603778	0.0
20	0.0	0.0	0.0	0.0040190618361371075	0.0
21	0.0	0.0	0.0	0.004593213527013837	0.0
22	0.0	0.0	0.0	0.005167365217890567	0.0
23	0.0	0.0	0.0	0.005167365217890567	0.0
24	0.0	0.0	0.0	0.007463971981397486	0.0
25	0.0	0.0	0.0	0.007463971981397486	0.0
26	0.0	0.0	0.0	0.009186427054027673	0.0
27	0.0	0.0	0.0	0.011483033817534592	0.0
28	0.0	0.0	0.0	0.024114371016822643	0.0
29	0.0	0.0	0.0	0.05282195556065913	0.0
30	0.0	0.0	0.0	0.07923293334098869	5.741516908767296E-4
31	0.0	0.0	0.0	0.13090658551989437	5.741516908767296E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACAT	20	0.0018376516	37.0	2
TCGCCGA	20	0.0018376516	37.0	1
GGGGTGG	20	0.0018376516	37.0	19
CTTATAC	1710	0.0	36.24269	1
TTATACA	1860	0.0	33.319893	2
GATACAC	45	1.07103915E-7	32.88889	3
TGATACA	45	1.07103915E-7	32.88889	2
TAGCTAC	40	1.5897695E-6	32.375	5
GTATTAG	40	1.5897695E-6	32.375	1
CCGTTTC	40	1.5897695E-6	32.375	11
GCTACCG	40	1.5897695E-6	32.375	7
TATTAGC	40	1.5897695E-6	32.375	2
CGTCTTC	710	0.0	32.049294	37
TATACAC	1980	0.0	31.300503	3
CGGAGAT	30	3.5870605E-4	30.833332	1
CAGCAGT	25	0.005483704	29.6	21
GATATTA	25	0.005483704	29.6	9
ATGATAT	25	0.005483704	29.6	7
GGATCAA	25	0.005483704	29.6	2
AGGCAGC	25	0.005483704	29.6	18
>>END_MODULE