FastQCFastQC Report
Fri 10 Feb 2017
ERR1631015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641590
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG58170.9066537820103181No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT51880.8086160943904985No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT49180.7665331442198289RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC31580.4922146542184261No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19690.30689381068906935No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18190.28351439392758615No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17500.27275986221730386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16950.26418740940476004No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC16050.25015975934787016No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC15820.2465749154444427RNA PCR Primer, Index 47 (95% over 23bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC15580.2428342087626054No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT15530.24205489487055595No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC15230.23737901151825933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.23176795149550336No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA14340.2235072242397793No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14230.22179273367727054No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT13290.20714163250674106No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12650.19716641468850823No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG11530.1797097835066008No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT11320.17643666515999315No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA11170.1740987234838448No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC10050.15664209230193737No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA9640.15025171838713197No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG9520.14838136504621333No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC9490.14791377671098366No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT9290.1447965211427859No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC8680.13528889165978275No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC8640.13466544054614316No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG8520.1327950872052245No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG8150.12702816440405867No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA8020.12500194828473013No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG7800.12157296715971258No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA7630.1189232999267445No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA7580.11814398603469506No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA7310.11393569101762807No Hit
CTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCT7180.11190947489829954No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA7090.11050670989261055No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG6970.1086363565516919No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC6900.10754531710282268No Hit
ACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGCAA6820.10629841487554358No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA6810.10614255209713368No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA6730.10489564986985457No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG6550.10209011985847659No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA6450.10053149207437773No Hit
TTACAGGACTTAACATTTCAAACATCCCACGTGGCTAGCAGGT6440.10037562929596784No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA6440.10037562929596784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG554.984031E-1033.6363641
CTTATAC6800.032.3751
GGTATCA3050.029.1147521
CGTCTTC2800.029.0714337
ATTACGG652.9849616E-928.4615382
TTATACA8200.027.073172
TATACAC8750.025.5828573
GTATCAA9950.025.2864321
CCGTCTT8350.024.59281337
GCCGTCT8550.024.01754436
TTACGGG952.8012437E-1023.3684223
CCGACCT502.7002985E-422.29
TCGCCAT2900.021.68965713
GTCGCCA3150.019.96825412
CGGCCTT1950.019.92307724
TGCCGTC10350.019.8405835
ATGCCGT10600.019.37264334
TCGGCCT2150.018.93023323
TACCCTC701.2181319E-418.55
GATACAC609.230751E-418.53
Produced by FastQC (version 0.11.5)