##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631015.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641590
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.993551956857182	31.0	30.0	34.0	28.0	34.0
2	30.997761810502034	31.0	31.0	34.0	27.0	34.0
3	30.389159743761592	31.0	30.0	34.0	26.0	34.0
4	34.63128321825465	35.0	35.0	37.0	32.0	37.0
5	34.735471251110525	35.0	35.0	37.0	32.0	37.0
6	34.96435885846101	35.0	35.0	37.0	32.0	37.0
7	34.99600679561714	36.0	35.0	37.0	32.0	37.0
8	35.035499306410635	36.0	35.0	37.0	32.0	37.0
9	36.73600741906825	38.0	35.0	39.0	32.0	39.0
10	36.50125157811063	38.0	35.0	39.0	32.0	39.0
11	36.58955563521875	38.0	35.0	39.0	32.0	39.0
12	36.51170529465858	38.0	35.0	39.0	32.0	39.0
13	36.61182998488131	38.0	35.0	39.0	32.0	39.0
14	37.630142302716685	39.0	37.0	40.0	32.0	41.0
15	37.64558518680154	39.0	37.0	40.0	32.0	41.0
16	37.64375535778301	39.0	37.0	40.0	32.0	41.0
17	37.602150906342054	39.0	37.0	40.0	32.0	41.0
18	37.648866098287066	39.0	37.0	40.0	32.0	41.0
19	37.68372480867844	39.0	37.0	40.0	32.0	41.0
20	37.682970432830935	39.0	37.0	40.0	32.0	41.0
21	37.64601380944217	39.0	37.0	40.0	32.0	41.0
22	37.56043735095622	39.0	37.0	40.0	32.0	41.0
23	37.55042940195452	39.0	37.0	40.0	32.0	41.0
24	37.49550647609844	39.0	36.0	40.0	32.0	41.0
25	37.4714272354619	39.0	36.0	40.0	32.0	41.0
26	37.34161224457987	39.0	36.0	40.0	32.0	41.0
27	37.26293583129413	39.0	36.0	40.0	32.0	41.0
28	37.26772393584688	39.0	36.0	40.0	32.0	41.0
29	37.1795819760283	39.0	36.0	40.0	31.0	41.0
30	37.119596627129475	39.0	36.0	40.0	31.0	41.0
31	37.08805000077931	39.0	36.0	40.0	31.0	41.0
32	36.99032248008853	39.0	36.0	40.0	31.0	41.0
33	36.97361399024299	39.0	36.0	40.0	31.0	41.0
34	36.89812964665908	39.0	36.0	40.0	31.0	41.0
35	36.89195436337848	39.0	36.0	40.0	31.0	41.0
36	36.87391012952197	39.0	36.0	40.0	30.0	41.0
37	36.81399959475677	39.0	36.0	40.0	30.0	41.0
38	36.75123677114668	39.0	35.0	40.0	30.0	41.0
39	36.69250767624184	39.0	35.0	40.0	30.0	41.0
40	36.63541046462694	38.0	35.0	40.0	30.0	41.0
41	36.59144157483751	38.0	35.0	40.0	30.0	41.0
42	36.561704515344694	38.0	35.0	40.0	30.0	41.0
43	35.83383936782057	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	0.0
12	4.0
13	4.0
14	4.0
15	2.0
16	5.0
17	16.0
18	18.0
19	33.0
20	92.0
21	164.0
22	303.0
23	714.0
24	1066.0
25	1977.0
26	3120.0
27	4793.0
28	6951.0
29	9965.0
30	13698.0
31	17928.0
32	23587.0
33	30893.0
34	40643.0
35	54005.0
36	76154.0
37	110356.0
38	158413.0
39	86678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.15442884084852	23.562711388893216	15.872597764927757	22.410262005330505
2	14.418398042363503	23.65747595816643	40.536167957730015	21.387958041740053
3	15.808382300222885	28.871085895977185	35.09562181455447	20.224909989245468
4	12.627846443990713	19.46211755170748	38.499820757804834	29.410215246496985
5	11.785252263906857	40.27759160834801	35.75398619055783	12.183169937187301
6	26.76989978023348	40.96962234448791	18.517589114543558	13.742888760735047
7	22.99318879658349	34.895494007076174	24.079708224878818	18.031608971461523
8	23.441606010068735	37.39568883554919	22.01748780373759	17.145217350644494
9	23.752084664661233	16.656275814772677	20.163655917330384	39.42798360323571
10	13.569881076700074	30.059383718574168	34.664193643915894	21.706541560809864
11	30.258108761046778	24.64346389438738	25.991365202076093	19.107062142489752
12	19.852553811624247	27.904424944279054	30.23847005096713	22.00455119312957
13	27.078508081485058	22.27746691812528	28.12294455960972	22.521080440779937
14	20.73645162798672	23.317850964011285	27.715675119624684	28.23002228837731
15	22.916192584049004	30.391215573808815	24.68741719789897	22.00517464424321
16	23.093252700322637	29.673935067566514	24.32488037531757	22.90793185679328
17	21.096494646113563	30.724450194049158	25.749310307205537	22.429744852631742
18	21.430664443024362	28.372169142287127	27.324303683037453	22.872862731651054
19	23.32579996571019	28.92641718231269	28.087719571689085	19.660063280288036
20	22.889540048940912	26.754157639614085	28.94013310681276	21.41616920463224
21	22.66899421749092	28.47223304602628	26.394893935379294	22.463878801103508
22	20.300971025109494	30.693901089480818	27.02457956015524	21.980548325254446
23	22.2808958992503	28.177184806496356	27.13960629062174	22.4023130036316
24	22.85041849156003	29.802210134197853	26.30979285836749	21.037578515874625
25	22.518430773546967	28.46599853488988	27.493103072055362	21.522467619507786
26	23.344191773562557	28.109228635109652	26.685733879892144	21.860845711435655
27	20.775417322589192	28.980969154756153	28.327592387661905	21.916021134992754
28	21.354759269938747	28.13354322854159	28.375909848968966	22.135787652550697
29	21.382970432830938	29.32994591561589	26.88508237347839	22.40200127807478
30	21.31922255646129	29.074019233466856	28.43934599978179	21.16741221029006
31	23.167599245624153	27.373244595458157	27.18184510357082	22.277311055346875
32	21.29428451191571	29.231284776882433	27.635561651522	21.83886905967986
33	22.57189170654156	27.26024408111099	28.713820352561605	21.454043859785845
34	20.63810221481008	29.212425380694835	27.22844807431537	22.92102433017971
35	21.41726024408111	28.525538116242462	29.09911314079085	20.95808849888558
36	22.92024501628766	28.241244408422823	25.96440094141118	22.874109633878334
37	22.677566670303463	27.213329384809615	27.815115572250193	22.29398837263673
38	22.45187736716595	27.423744135662965	28.048753877086614	22.075624620084476
39	22.957496220327624	27.537056375566948	27.67889150391995	21.826555900185475
40	21.5006468305304	26.578812013902958	29.922692061908695	21.997849093657944
41	21.423183029660684	28.239374055081907	27.552019202294296	22.78542371296311
42	22.346514128960862	26.769743917455074	28.948393834068487	21.93534811951558
43	21.873626459265264	26.823204850449667	28.836016770834956	22.467151919450114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	13.5
2	17.0
3	64.5
4	112.0
5	112.0
6	134.0
7	156.0
8	155.5
9	155.0
10	289.5
11	424.0
12	424.0
13	610.5
14	797.0
15	2161.0
16	3525.0
17	3973.0
18	4421.0
19	4421.0
20	4912.5
21	5404.0
22	7113.5
23	8823.0
24	10938.5
25	13054.0
26	13054.0
27	16029.5
28	19005.0
29	25101.5
30	31198.0
31	31941.5
32	32685.0
33	32685.0
34	35027.5
35	37370.0
36	38672.5
37	39975.0
38	44749.5
39	49524.0
40	49524.0
41	53136.0
42	56748.0
43	50419.0
44	44090.0
45	45301.5
46	46513.0
47	46513.0
48	45652.5
49	44792.0
50	45651.5
51	46511.0
52	51757.5
53	57004.0
54	57004.0
55	42558.5
56	28113.0
57	28182.0
58	28251.0
59	22603.0
60	16955.0
61	16955.0
62	13272.0
63	9589.0
64	7833.5
65	6078.0
66	5084.5
67	4091.0
68	4091.0
69	3259.0
70	2427.0
71	2065.5
72	1704.0
73	1363.0
74	1022.0
75	1022.0
76	771.0
77	520.0
78	428.5
79	337.0
80	217.0
81	97.0
82	97.0
83	73.5
84	50.0
85	38.5
86	27.0
87	20.0
88	13.0
89	13.0
90	7.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	641590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.26661152859774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.50469824512594	37.799747362748946
2	9.464507691189835	8.568523859330318
3	2.4839200371618864	3.3731593017092156
4	1.1305540735652737	2.0470540824061185
5	0.6270416036604801	1.419202434258395
6	0.4181333792206878	1.1356488746593632
7	0.32042137983901875	1.0153073088641087
8	0.2255545627484956	0.8168072616351101
9	0.18195708681466338	0.7412922687343232
>10	1.3092059542843975	11.782812404869565
>50	0.15330285167546906	4.9273594512836825
>100	0.15919911484389593	14.949756760001376
>500	0.013873561206439735	4.282865098257483
>1k	0.006936780603219867	5.412653479322069
>5k	6.936780603219866E-4	1.7278100519199238
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	5817	0.9066537820103181	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	5188	0.8086160943904985	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	4918	0.7665331442198289	RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	3158	0.4922146542184261	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1969	0.30689381068906935	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1819	0.28351439392758615	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1750	0.27275986221730386	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1695	0.26418740940476004	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1605	0.25015975934787016	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	1582	0.2465749154444427	RNA PCR Primer, Index 47 (95% over 23bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	1558	0.2428342087626054	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1553	0.24205489487055595	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1523	0.23737901151825933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1487	0.23176795149550336	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1434	0.2235072242397793	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1423	0.22179273367727054	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1329	0.20714163250674106	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1265	0.19716641468850823	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1153	0.1797097835066008	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1132	0.17643666515999315	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1117	0.1740987234838448	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1005	0.15664209230193737	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	964	0.15025171838713197	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	952	0.14838136504621333	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	949	0.14791377671098366	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	929	0.1447965211427859	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	868	0.13528889165978275	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	864	0.13466544054614316	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	852	0.1327950872052245	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	815	0.12702816440405867	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	802	0.12500194828473013	No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	780	0.12157296715971258	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	763	0.1189232999267445	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	758	0.11814398603469506	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	731	0.11393569101762807	No Hit
CTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCT	718	0.11190947489829954	No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA	709	0.11050670989261055	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	697	0.1086363565516919	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	690	0.10754531710282268	No Hit
ACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGCAA	682	0.10629841487554358	No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA	681	0.10614255209713368	No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA	673	0.10489564986985457	No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG	655	0.10209011985847659	No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA	645	0.10053149207437773	No Hit
TTACAGGACTTAACATTTCAAACATCCCACGTGGCTAGCAGGT	644	0.10037562929596784	No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA	644	0.10037562929596784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5586277840988794E-4	0.0
5	0.0	0.0	0.0	1.5586277840988794E-4	0.0
6	0.0	0.0	0.0	1.5586277840988794E-4	0.0
7	0.0	0.0	0.0	1.5586277840988794E-4	0.0
8	0.0	0.0	0.0	1.5586277840988794E-4	0.0
9	0.0	0.0	0.0	1.5586277840988794E-4	0.0
10	0.0	0.0	0.0	1.5586277840988794E-4	0.0
11	0.0	0.0	0.0	3.117255568197759E-4	0.0
12	0.0	0.0	0.0	3.117255568197759E-4	0.0
13	0.0	0.0	0.0	3.117255568197759E-4	0.0
14	0.0	0.0	0.0	3.117255568197759E-4	0.0
15	0.0	0.0	0.0	3.117255568197759E-4	0.0
16	0.0	0.0	0.0	3.117255568197759E-4	0.0
17	0.0	0.0	0.0	3.117255568197759E-4	0.0
18	0.0	0.0	0.0	3.117255568197759E-4	0.0
19	0.0	0.0	0.0	4.675883352296638E-4	0.0
20	0.0	0.0	0.0	4.675883352296638E-4	0.0
21	0.0	0.0	0.0	7.793138920494396E-4	0.0
22	0.0	0.0	0.0	7.793138920494396E-4	0.0
23	0.0	0.0	0.0	0.0012469022272791036	0.0
24	0.0	0.0	0.0	0.0012469022272791036	0.0
25	0.0	0.0	0.0	0.0014027650056889914	0.0
26	0.0	0.0	0.0	0.0014027650056889914	0.0
27	0.0	0.0	0.0	0.0017144905625087674	0.0
28	0.0	0.0	0.0	0.004675883352296638	0.0
29	0.0	0.0	0.0	0.011845571159151483	0.0
30	0.0	0.0	0.0	0.02571735843763151	0.0
31	0.0	0.0	0.0	0.055175423557100325	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	55	4.984031E-10	33.636364	1
CTTATAC	680	0.0	32.375	1
GGTATCA	305	0.0	29.114752	1
CGTCTTC	280	0.0	29.07143	37
ATTACGG	65	2.9849616E-9	28.461538	2
TTATACA	820	0.0	27.07317	2
TATACAC	875	0.0	25.582857	3
GTATCAA	995	0.0	25.286432	1
CCGTCTT	835	0.0	24.592813	37
GCCGTCT	855	0.0	24.017544	36
TTACGGG	95	2.8012437E-10	23.368422	3
CCGACCT	50	2.7002985E-4	22.2	9
TCGCCAT	290	0.0	21.689657	13
GTCGCCA	315	0.0	19.968254	12
CGGCCTT	195	0.0	19.923077	24
TGCCGTC	1035	0.0	19.84058	35
ATGCCGT	1060	0.0	19.372643	34
TCGGCCT	215	0.0	18.930233	23
TACCCTC	70	1.2181319E-4	18.5	5
GATACAC	60	9.230751E-4	18.5	3
>>END_MODULE