FastQCFastQC Report
Fri 10 Feb 2017
ERR1631014.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631014.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631204
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT107561.7040449680293537No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG94951.504268033789393No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT88741.4058846268401342TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC50580.8013257203693259No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC30150.47765856997103945RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC28690.4545281715578482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16120.2553849468634546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.24271075595211689No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC14110.22354104219871862No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13190.20896572265068028No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12590.19946007946717703No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG12140.19233084707954956No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC11550.18298363128243803No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11510.18234992173687112No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10480.16603190093852385No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA10250.16238807105151423No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10000.15842738639172121No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9830.15573412082306196No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA9600.15209029093605236No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8540.1352969879785299No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8010.1269003364997687No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT7960.1261081995678101No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC7880.12484078047667632No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG7870.1246823530902846No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT7680.1216722327488419No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT7240.11470142774760617No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC7050.11169130740616347No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6930.10979017876946282No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA6930.10979017876946282No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG6860.10868118706472076No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG6470.10250251899544362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12700.032.629921
CGTCTTC4400.031.95454637
AACGCAC303.5976557E-430.83333432
TTATACA13750.030.6763652
GATTACG551.8530045E-830.2727281
CTATCGC250.00549448929.63
GAACGCA250.00549448929.631
ACGTATA250.00549448929.66
TATACAC14450.028.9342563
CCGTCTT15200.026.53289637
GCCGTCT15500.026.01935636
TTACGGG601.3358112E-624.6666683
GGTATCA3250.023.3384631
GTATCAA8550.023.1520461
GTGATAC400.001930227623.1250023
CGGCCTT1450.021.68965524
TGCCGTC18750.021.50933535
CGCGATA806.9498856E-720.812534
GACCGTG450.003823926420.5555557
ATGCCGT19850.020.22418234
Produced by FastQC (version 0.11.5)