##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631204
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487796338426246	31.0	31.0	34.0	28.0	34.0
2	31.508390314383306	31.0	31.0	34.0	30.0	34.0
3	30.949859316480882	31.0	31.0	34.0	27.0	34.0
4	35.07994404344713	37.0	35.0	37.0	32.0	37.0
5	35.295715806617196	37.0	35.0	37.0	33.0	37.0
6	35.48498742086552	37.0	35.0	37.0	33.0	37.0
7	35.49872465953955	37.0	35.0	37.0	33.0	37.0
8	35.53221779329662	37.0	35.0	37.0	33.0	37.0
9	37.304251874195984	39.0	37.0	39.0	34.0	39.0
10	37.143492436676574	39.0	37.0	39.0	33.0	39.0
11	37.20386911363046	39.0	37.0	39.0	33.0	39.0
12	37.13502290860007	39.0	37.0	39.0	33.0	39.0
13	37.20214700794038	39.0	37.0	39.0	33.0	39.0
14	38.34830894607766	40.0	38.0	41.0	34.0	41.0
15	38.36001039283655	40.0	38.0	41.0	34.0	41.0
16	38.359083592626156	40.0	38.0	41.0	34.0	41.0
17	38.32417728658247	40.0	38.0	41.0	33.0	41.0
18	38.37154073801813	40.0	38.0	41.0	34.0	41.0
19	38.397671751129586	40.0	38.0	41.0	34.0	41.0
20	38.37784773227039	40.0	38.0	41.0	34.0	41.0
21	38.349387836578984	40.0	38.0	41.0	34.0	41.0
22	38.27892725648127	40.0	38.0	41.0	34.0	41.0
23	38.28015348445194	40.0	38.0	41.0	34.0	41.0
24	38.21321474515371	40.0	38.0	41.0	34.0	41.0
25	38.20391505757251	40.0	38.0	41.0	34.0	41.0
26	38.10298413825007	40.0	37.0	41.0	33.0	41.0
27	38.023260308870036	40.0	37.0	41.0	33.0	41.0
28	38.03709260397589	40.0	37.0	41.0	33.0	41.0
29	37.9700556396981	40.0	37.0	41.0	33.0	41.0
30	37.9167923523932	40.0	37.0	41.0	33.0	41.0
31	37.88869842396436	40.0	37.0	41.0	33.0	41.0
32	37.80819354756941	40.0	37.0	41.0	33.0	41.0
33	37.78449439483907	40.0	37.0	41.0	33.0	41.0
34	37.72621371220715	40.0	37.0	41.0	33.0	41.0
35	37.740773189016544	40.0	37.0	41.0	33.0	41.0
36	37.72098085563463	40.0	37.0	41.0	33.0	41.0
37	37.657928340124585	40.0	37.0	41.0	33.0	41.0
38	37.625548950893844	40.0	37.0	41.0	33.0	41.0
39	37.563679887960156	40.0	37.0	41.0	32.0	41.0
40	37.478586954455295	40.0	37.0	41.0	32.0	41.0
41	37.45678100899234	40.0	37.0	41.0	32.0	41.0
42	37.41578316994189	40.0	37.0	41.0	32.0	41.0
43	36.767249573830334	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	15.0
11	16.0
12	12.0
13	9.0
14	7.0
15	12.0
16	3.0
17	9.0
18	16.0
19	22.0
20	46.0
21	90.0
22	208.0
23	395.0
24	687.0
25	1196.0
26	2020.0
27	3082.0
28	4808.0
29	6944.0
30	9788.0
31	13246.0
32	17240.0
33	23070.0
34	30525.0
35	41521.0
36	57665.0
37	86636.0
38	152687.0
39	179219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.12825013783183	23.31623373742879	15.564857003441043	23.990659121298343
2	13.35748822884519	22.620420656396348	41.78142090354307	22.24067021121539
3	14.386315676073028	28.961318369338596	35.56615610800946	21.086209846578917
4	11.584210492962656	19.617587974727662	37.67561675781522	31.12258477449446
5	11.718715344009228	39.36366055981901	35.99359319649432	12.92403089967744
6	26.80211152020583	40.202058288604	18.389142020646258	14.606688170543913
7	22.72118047414148	34.51910951134657	23.408438476308767	19.35127153820318
8	23.63689076748563	36.65692866331646	22.458507867504007	17.247672701693904
9	22.798968320859817	16.120461847516808	19.78203560180227	41.2985342298211
10	13.609704627980811	29.697372006514534	34.22823682993137	22.464686535573286
11	30.01739532702581	23.780109124783746	25.98462620642455	20.217869341765894
12	19.347152426156995	27.483824563849407	29.64017972002712	23.528843289966478
13	27.703246494001938	21.269985614793317	27.80337260220151	23.22339528900324
14	20.990994987357496	23.17951090297273	26.656199897339057	29.17329421233072
15	23.28137971242261	29.668221367418457	23.677289751015522	23.373109169143415
16	24.355992674317655	28.39129663310118	23.683309991698405	23.569400700882756
17	20.567835438305206	31.431518177958313	25.037547290574835	22.96309909316164
18	22.174922845862827	26.8849690432887	26.870235296354267	24.069872814494207
19	22.79564134574559	28.579825222907335	28.01439788087528	20.610135550471796
20	22.378977319535366	27.567791078637015	26.873720698854886	23.17951090297273
21	24.28295764919107	26.978758056032596	26.108833277355657	22.629451017420678
22	21.27125303388445	31.429775476708006	25.881173123110752	21.417798366296793
23	22.10141253857707	27.246341911648216	26.311303477164277	24.34094207261044
24	23.436955405859276	29.979055899519015	26.751097901787695	19.832890792834014
25	22.040417994816256	28.501086811870646	27.411740103041172	22.046755090271926
26	24.72100937256418	26.8352228439617	27.40017490383458	21.043592879639547
27	21.087635693056445	30.649203743955994	27.307494882795417	20.95566568019214
28	21.535509914385838	26.667765096545647	28.222254611821217	23.57447037724729
29	22.468013510687513	28.094403711003096	28.074600287704133	21.362982490605255
30	20.229909823131667	28.864677663639647	28.382583126849642	22.522829386379048
31	24.377221944094142	27.668075614222975	26.082217476441848	21.872484965241032
32	23.125011882053983	27.259016102559553	28.808594368856976	20.80737764652949
33	21.91098282013422	26.610572810058237	28.740153737935753	22.738290631871788
34	20.306430250758865	30.867199827631005	27.101697707872574	21.724672213737556
35	21.214852884328998	26.42600490491188	31.90521606326956	20.45392614748956
36	24.054346930627815	27.01551320967548	25.44106184371455	23.489078015982155
37	21.902744596041853	27.36104333939582	28.090443026343305	22.645769038219022
38	22.004613405491728	26.185353704982855	28.51692955050982	23.293103339015595
39	23.583976020430796	26.419667809456215	27.309554438818513	22.68680173129448
40	20.846192356195463	25.43329890178136	31.679140182888577	22.041368559134604
41	21.672232748841896	27.435662638386322	26.85724425067015	24.034860362101636
42	22.51189789671802	25.791503222413038	29.93612207780686	21.760476803062083
43	22.122800235739952	25.641789342272865	29.56255030069518	22.672860121292008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	60.5
4	105.0
5	105.0
6	133.0
7	161.0
8	162.5
9	164.0
10	273.0
11	382.0
12	382.0
13	589.5
14	797.0
15	2103.5
16	3410.0
17	3770.5
18	4131.0
19	4131.0
20	4399.5
21	4668.0
22	6095.5
23	7523.0
24	9372.0
25	11221.0
26	11221.0
27	14196.5
28	17172.0
29	22181.0
30	27190.0
31	28591.0
32	29992.0
33	29992.0
34	32786.5
35	35581.0
36	37163.0
37	38745.0
38	42534.0
39	46323.0
40	46323.0
41	49050.0
42	51777.0
43	47402.5
44	43028.0
45	43734.5
46	44441.0
47	44441.0
48	44938.5
49	45436.0
50	54772.5
51	64109.0
52	53251.0
53	42393.0
54	42393.0
55	42587.0
56	42781.0
57	35365.0
58	27949.0
59	21011.5
60	14074.0
61	14074.0
62	12145.5
63	10217.0
64	8450.5
65	6684.0
66	5512.0
67	4340.0
68	4340.0
69	3514.0
70	2688.0
71	2236.0
72	1784.0
73	1386.0
74	988.0
75	988.0
76	725.5
77	463.0
78	363.5
79	264.0
80	192.0
81	120.0
82	120.0
83	83.0
84	46.0
85	35.0
86	24.0
87	16.0
88	8.0
89	8.0
90	6.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	631204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.04929540316967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.23629059699134	43.00203282353054
2	9.571254362737967	9.772115826845738
3	2.4484878075775645	3.7498073214025895
4	0.9808381036730007	2.0028437638835106
5	0.5374419108656252	1.3718015434911643
6	0.36019788791221197	1.1032709030176984
7	0.2596428992908752	0.9278210945664724
8	0.18435830743001483	0.7529089356818557
9	0.135992301259899	0.6248080043616083
>10	1.0410070478028048	10.4265344231732
>50	0.12394868319739477	4.396025696373014
>100	0.1072156081656393	10.579567925025145
>500	0.008366535897318646	2.939278582758551
>1k	0.0037184603988082866	2.943866015022692
>5k	9.296150997020716E-4	3.7058543328284044
>10k+	3.0987169990069055E-4	1.7014628080378333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	10756	1.7040449680293537	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	9495	1.504268033789393	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	8874	1.4058846268401342	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	5058	0.8013257203693259	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3015	0.47765856997103945	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	2869	0.4545281715578482	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1612	0.2553849468634546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1532	0.24271075595211689	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1411	0.22354104219871862	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1319	0.20896572265068028	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1259	0.19946007946717703	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1214	0.19233084707954956	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1155	0.18298363128243803	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1151	0.18234992173687112	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1048	0.16603190093852385	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1025	0.16238807105151423	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1000	0.15842738639172121	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	983	0.15573412082306196	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	960	0.15209029093605236	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	854	0.1352969879785299	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	801	0.1269003364997687	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	796	0.1261081995678101	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	788	0.12484078047667632	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	787	0.1246823530902846	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	768	0.1216722327488419	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	724	0.11470142774760617	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	705	0.11169130740616347	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	693	0.10979017876946282	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	693	0.10979017876946282	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	686	0.10868118706472076	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	647	0.10250251899544362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.584273863917212E-4	0.0	0.0	0.0	0.0
6	1.584273863917212E-4	0.0	0.0	0.0	0.0
7	1.584273863917212E-4	0.0	0.0	0.0	0.0
8	1.584273863917212E-4	0.0	0.0	0.0	0.0
9	1.584273863917212E-4	0.0	0.0	0.0	0.0
10	1.584273863917212E-4	0.0	0.0	1.584273863917212E-4	0.0
11	1.584273863917212E-4	0.0	0.0	1.584273863917212E-4	0.0
12	1.584273863917212E-4	0.0	0.0	1.584273863917212E-4	0.0
13	1.584273863917212E-4	1.584273863917212E-4	0.0	1.584273863917212E-4	0.0
14	1.584273863917212E-4	1.584273863917212E-4	0.0	1.584273863917212E-4	0.0
15	1.584273863917212E-4	1.584273863917212E-4	0.0	1.584273863917212E-4	0.0
16	1.584273863917212E-4	1.584273863917212E-4	0.0	3.168547727834424E-4	0.0
17	1.584273863917212E-4	1.584273863917212E-4	0.0	4.7528215917516366E-4	0.0
18	1.584273863917212E-4	1.584273863917212E-4	0.0	4.7528215917516366E-4	0.0
19	1.584273863917212E-4	1.584273863917212E-4	0.0	4.7528215917516366E-4	0.0
20	1.584273863917212E-4	1.584273863917212E-4	0.0	4.7528215917516366E-4	0.0
21	1.584273863917212E-4	1.584273863917212E-4	0.0	7.921369319586061E-4	0.0
22	1.584273863917212E-4	1.584273863917212E-4	0.0	9.505643183503273E-4	0.0
23	1.584273863917212E-4	1.584273863917212E-4	0.0	0.0015842738639172123	0.0
24	1.584273863917212E-4	1.584273863917212E-4	0.0	0.0026932655686592605	0.0
25	1.584273863917212E-4	1.584273863917212E-4	0.0	0.0026932655686592605	0.0
26	1.584273863917212E-4	1.584273863917212E-4	0.0	0.0028516929550509817	0.0
27	1.584273863917212E-4	1.584273863917212E-4	0.0	0.0033269751142261457	0.0
28	3.168547727834424E-4	1.584273863917212E-4	0.0	0.0071292323876274545	0.0
29	3.168547727834424E-4	1.584273863917212E-4	0.0	0.014575319548038352	0.0
30	3.168547727834424E-4	1.584273863917212E-4	0.0	0.02709108307298433	0.0
31	3.168547727834424E-4	1.584273863917212E-4	0.0	0.06384623671586365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1270	0.0	32.62992	1
CGTCTTC	440	0.0	31.954546	37
AACGCAC	30	3.5976557E-4	30.833334	32
TTATACA	1375	0.0	30.676365	2
GATTACG	55	1.8530045E-8	30.272728	1
CTATCGC	25	0.005494489	29.6	3
GAACGCA	25	0.005494489	29.6	31
ACGTATA	25	0.005494489	29.6	6
TATACAC	1445	0.0	28.934256	3
CCGTCTT	1520	0.0	26.532896	37
GCCGTCT	1550	0.0	26.019356	36
TTACGGG	60	1.3358112E-6	24.666668	3
GGTATCA	325	0.0	23.338463	1
GTATCAA	855	0.0	23.152046	1
GTGATAC	40	0.0019302276	23.125002	3
CGGCCTT	145	0.0	21.689655	24
TGCCGTC	1875	0.0	21.509335	35
CGCGATA	80	6.9498856E-7	20.8125	34
GACCGTG	45	0.0038239264	20.555555	7
ATGCCGT	1985	0.0	20.224182	34
>>END_MODULE