FastQCFastQC Report
Fri 10 Feb 2017
ERR1631013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382041
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG56171.4702610452804803No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT38551.0090540020573708Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT35070.9179643022607521No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC29590.7745242002821686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14600.38215793592834274No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC14090.3688085833719418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.3682850793501221No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC11930.3122701490154198Illumina PCR Primer Index 11 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG6780.17746786339686055No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5390.14108433388039504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5390.14108433388039504No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA5010.13113775746582174No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4810.12590271724762525No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4670.12223818909488772No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA4490.11752665289851089No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4450.1164796448548716No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4190.10967409257121617No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT4100.10731832447302776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT200.001840398337.022
GATTACG352.384166E-531.7142851
GCTCACG303.5950207E-430.83333221
CTTATAC5600.030.0624981
GGTATCA1850.030.01
CGTCTTC2150.027.53488337
CTCACGC358.8585145E-426.4285722
GTATCAA6950.026.086331
TTATACA6550.025.9847322
TATACAC6650.025.8721793
CCGTCTT6400.025.14843837
GTCGCCA751.3676981E-824.66666612
GCCGTCT6650.024.20300736
TCGCCAT855.1579264E-821.76470613
ACACTTA705.0872222E-621.1428576
AGCGAAC450.003821203320.55555522
CACTTAC555.134459E-420.1818187
TGCCGTC8000.020.1187535
ACAATAC656.886722E-519.9230773
ATACTGG759.239238E-619.7333346
Produced by FastQC (version 0.11.5)