##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631013.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382041
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.431618595909864	31.0	30.0	33.0	27.0	34.0
2	30.429846534796003	31.0	30.0	33.0	27.0	34.0
3	29.81660868859625	31.0	30.0	34.0	25.0	34.0
4	34.09613104352674	35.0	33.0	37.0	28.0	37.0
5	33.95470643203216	35.0	33.0	37.0	29.0	37.0
6	34.238403731536664	35.0	35.0	37.0	30.0	37.0
7	34.29471705916381	35.0	35.0	37.0	30.0	37.0
8	34.352629691577604	35.0	35.0	37.0	30.0	37.0
9	35.96051209163414	37.0	35.0	39.0	32.0	39.0
10	35.626966739171976	37.0	35.0	39.0	30.0	39.0
11	35.80447124785036	37.0	35.0	39.0	30.0	39.0
12	35.68538978800704	37.0	35.0	39.0	30.0	39.0
13	35.82923560560254	37.0	35.0	39.0	30.0	39.0
14	36.673058650773086	38.0	36.0	40.0	31.0	41.0
15	36.67986682057685	38.0	36.0	40.0	31.0	41.0
16	36.68706500087687	38.0	36.0	40.0	31.0	41.0
17	36.61929740525232	38.0	36.0	40.0	31.0	41.0
18	36.699013980174904	38.0	36.0	40.0	31.0	41.0
19	36.743278862739864	38.0	36.0	40.0	31.0	41.0
20	36.72686962917592	38.0	36.0	40.0	31.0	41.0
21	36.68829000028793	38.0	36.0	40.0	31.0	41.0
22	36.551815119319656	38.0	35.0	40.0	30.0	41.0
23	36.57268984218971	38.0	35.0	40.0	30.0	41.0
24	36.493693085297124	38.0	35.0	40.0	30.0	41.0
25	36.45078407814868	38.0	35.0	40.0	30.0	41.0
26	36.28688282147728	38.0	35.0	40.0	30.0	41.0
27	36.1615690462542	38.0	35.0	40.0	30.0	41.0
28	36.143662591187855	38.0	35.0	40.0	30.0	41.0
29	36.05532652254601	38.0	34.0	40.0	30.0	41.0
30	35.95260456338456	38.0	34.0	40.0	29.0	41.0
31	35.927683154425836	38.0	34.0	40.0	29.0	41.0
32	35.803591761093706	38.0	34.0	40.0	29.0	41.0
33	35.741915134763026	38.0	34.0	40.0	29.0	41.0
34	35.631696598009114	38.0	34.0	40.0	29.0	41.0
35	35.59173753602362	38.0	34.0	40.0	28.0	41.0
36	35.59406974644083	38.0	34.0	40.0	28.0	41.0
37	35.50905007577721	38.0	34.0	40.0	28.0	41.0
38	35.428511599540364	38.0	34.0	40.0	27.0	41.0
39	35.36752861603859	38.0	34.0	40.0	27.0	41.0
40	35.244785245562646	38.0	34.0	40.0	27.0	41.0
41	35.190194246167295	38.0	33.0	40.0	27.0	41.0
42	35.120400689978304	38.0	33.0	40.0	27.0	41.0
43	34.31434584246193	37.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	5.0
12	5.0
13	9.0
14	5.0
15	4.0
16	7.0
17	13.0
18	30.0
19	47.0
20	118.0
21	222.0
22	421.0
23	791.0
24	1225.0
25	2093.0
26	3146.0
27	4610.0
28	6472.0
29	9063.0
30	12066.0
31	15663.0
32	20134.0
33	26029.0
34	33453.0
35	43996.0
36	56272.0
37	66098.0
38	59941.0
39	20092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.280553657853474	24.49763245306132	16.071835221874093	22.149978667211112
2	15.023518418180249	23.846655201928588	39.539735264016166	21.590091115874998
3	16.542727089500865	29.45390677963883	33.84793778678205	20.155428344078253
4	12.5842514285116	19.808606929622737	38.12496564504857	29.482175996817094
5	11.756853322025647	40.12239524030144	35.43101394876466	12.689737488908259
6	26.437738357924935	41.02308390984214	18.21401367916009	14.325164053072836
7	22.98208830989344	34.86589135721035	23.649555937713494	18.502464395182717
8	24.033284385707294	36.252391758999686	22.44523493551739	17.269088919775626
9	23.65452922592078	15.95666433707377	20.072452956619838	40.316353480385615
10	13.700362003031088	29.640797715428448	34.55728573634767	22.101554545192794
11	30.887260791381028	24.234048178075128	25.23289385170702	19.645797178836826
12	20.06826492444528	27.739954612201306	30.049654356469595	22.142126106883815
13	28.42417436871959	21.841896550370247	27.212786062228922	22.52114301868124
14	21.09590331927725	23.46135624186933	27.7370753400813	27.70566509877212
15	23.334930020599884	29.99416293015671	24.042707458100047	22.628199591143357
16	22.991511382286195	28.94479911841923	24.386649600435558	23.67703989885902
17	20.566902505228494	30.640166893082153	25.894864687297957	22.89806591439139
18	21.92722770592685	27.11174978601773	27.49940451417518	23.46161799388024
19	23.16295894943213	28.5123847963962	28.470504474650625	19.854151779521047
20	22.25520297559686	27.803822102863307	27.40150926209491	22.53946565944493
21	23.20431576715588	27.107038249821354	26.884549040548006	22.80409694247476
22	21.219450268426687	30.835433893220888	26.91805329794446	21.027062540407968
23	21.92722770592685	27.06882245622852	27.510921602655213	23.493028235189417
24	22.67479144908531	28.786700903829693	28.338319709141164	20.200187937943834
25	22.51250520232122	28.163469365853405	26.951557555340923	22.37246787648446
26	21.934295010221415	26.808640957384156	28.639334521687466	22.617729510706965
27	21.772008763457325	30.055936404731433	27.255451640007223	20.916603191804022
28	20.09025209336171	26.866226399784317	28.681738347454854	24.361783159399124
29	22.828963383511194	27.17535552466882	28.695349452022167	21.30033163979782
30	21.328077352954264	28.238330440973613	27.901455602932668	22.532136603139456
31	21.79059315623192	26.49480029630328	28.36266264615578	23.35194390130902
32	23.972296167165304	27.389992173614875	27.758277252964998	20.879434406254827
33	20.475289301410058	26.56102355506346	29.421187778275105	23.542499365251373
34	20.854044461196573	28.844286346229854	28.534895469334444	21.76677372323913
35	22.08663468057093	26.893710360929845	30.448040917074344	20.571614041424873
36	22.29943906544062	27.05704361573758	26.31366790475368	24.329849414068125
37	22.063338751599957	27.19001363727977	28.65556314636387	22.0910844647564
38	22.075641096112722	26.54741245049615	28.412919032250468	22.964027421140663
39	23.378380854410914	27.103635473679528	27.652791192568337	21.86519247934122
40	20.96371855376779	26.336963833724653	31.18304056370913	21.516277048798425
41	21.86833350347214	27.788378734219627	27.319580882680132	23.023706879628104
42	21.95942320326876	26.21760491674977	29.884488837585494	21.938483042395973
43	22.435811863124638	26.053224653898404	29.058922995175912	22.452040487801046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	8.0
2	9.0
3	40.5
4	72.0
5	72.0
6	106.5
7	141.0
8	145.5
9	150.0
10	231.5
11	313.0
12	313.0
13	507.0
14	701.0
15	1719.5
16	2738.0
17	3047.5
18	3357.0
19	3357.0
20	3552.0
21	3747.0
22	4559.0
23	5371.0
24	6796.5
25	8222.0
26	8222.0
27	10064.0
28	11906.0
29	14367.5
30	16829.0
31	18055.0
32	19281.0
33	19281.0
34	20804.0
35	22327.0
36	23111.5
37	23896.0
38	25481.5
39	27067.0
40	27067.0
41	28187.0
42	29307.0
43	27530.0
44	25753.0
45	26033.5
46	26314.0
47	26314.0
48	25553.0
49	24792.0
50	25001.0
51	25210.0
52	29338.0
53	33466.0
54	33466.0
55	25860.5
56	18255.0
57	19515.0
58	20775.0
59	16950.0
60	13125.0
61	13125.0
62	9984.0
63	6843.0
64	5750.5
65	4658.0
66	3855.0
67	3052.0
68	3052.0
69	2384.0
70	1716.0
71	1467.5
72	1219.0
73	965.5
74	712.0
75	712.0
76	540.5
77	369.0
78	284.5
79	200.0
80	139.5
81	79.0
82	79.0
83	55.5
84	32.0
85	23.0
86	14.0
87	9.0
88	4.0
89	4.0
90	7.0
91	10.0
92	6.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	382041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.72388962245788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.94295714525207	53.4023842276083
2	6.984396875750938	8.482394899250794
3	1.8105567102148297	3.298321374788573
4	0.8045782308322803	1.9542847872676732
5	0.4649406960864021	1.4116503755069711
6	0.3109806118925887	1.133037141077398
7	0.21991840078542751	0.9348010486669228
8	0.17671438634199454	0.8584627912745325
9	0.13916153380944024	0.7605386656862752
>10	0.9806838985337508	11.748099987625775
>50	0.11553494729525925	4.865200292130689
>100	0.044403356609694876	4.9560383067279705
>500	0.0017244021984347524	0.5908397664422688
>1k	0.0030177038472608167	4.133522335898725
>5k	4.311005496086881E-4	1.4704240000470639
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	5617	1.4702610452804803	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	3855	1.0090540020573708	Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	3507	0.9179643022607521	No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	2959	0.7745242002821686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1460	0.38215793592834274	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	1409	0.3688085833719418	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1407	0.3682850793501221	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	1193	0.3122701490154198	Illumina PCR Primer Index 11 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	678	0.17746786339686055	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	539	0.14108433388039504	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	539	0.14108433388039504	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	501	0.13113775746582174	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	481	0.12590271724762525	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	467	0.12223818909488772	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	449	0.11752665289851089	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	445	0.1164796448548716	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	419	0.10967409257121617	No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT	410	0.10731832447302776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.617520109098238E-4	0.0
13	0.0	0.0	0.0	5.235040218196476E-4	0.0
14	0.0	0.0	0.0	5.235040218196476E-4	0.0
15	0.0	0.0	0.0	5.235040218196476E-4	0.0
16	0.0	0.0	0.0	5.235040218196476E-4	0.0
17	0.0	0.0	0.0	7.852560327294715E-4	0.0
18	0.0	0.0	0.0	7.852560327294715E-4	0.0
19	0.0	0.0	0.0	7.852560327294715E-4	0.0
20	0.0	0.0	0.0	7.852560327294715E-4	0.0
21	0.0	0.0	0.0	0.001308760054549119	0.0
22	0.0	0.0	0.0	0.001308760054549119	0.0
23	0.0	0.0	0.0	0.001570512065458943	0.0
24	0.0	0.0	0.0	0.0020940160872785904	0.0
25	0.0	0.0	0.0	0.0020940160872785904	0.0
26	0.0	0.0	0.0	0.0020940160872785904	0.0
27	0.0	0.0	0.0	0.0020940160872785904	0.0
28	0.0	0.0	0.0	0.005758544240016124	0.0
29	0.0	0.0	0.0	0.014658112610950133	0.0
30	0.0	0.0	0.0	0.026436953101892206	0.0
31	0.0	0.0	0.0	0.054967922291063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGAAT	20	0.0018403983	37.0	22
GATTACG	35	2.384166E-5	31.714285	1
GCTCACG	30	3.5950207E-4	30.833332	21
CTTATAC	560	0.0	30.062498	1
GGTATCA	185	0.0	30.0	1
CGTCTTC	215	0.0	27.534883	37
CTCACGC	35	8.8585145E-4	26.42857	22
GTATCAA	695	0.0	26.08633	1
TTATACA	655	0.0	25.984732	2
TATACAC	665	0.0	25.872179	3
CCGTCTT	640	0.0	25.148438	37
GTCGCCA	75	1.3676981E-8	24.666666	12
GCCGTCT	665	0.0	24.203007	36
TCGCCAT	85	5.1579264E-8	21.764706	13
ACACTTA	70	5.0872222E-6	21.142857	6
AGCGAAC	45	0.0038212033	20.555555	22
CACTTAC	55	5.134459E-4	20.181818	7
TGCCGTC	800	0.0	20.11875	35
ACAATAC	65	6.886722E-5	19.923077	3
ATACTGG	75	9.239238E-6	19.733334	6
>>END_MODULE