FastQCFastQC Report
Fri 10 Feb 2017
ERR1631012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403033
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT82472.046234427453831RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT81162.0137308855602396No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG80261.9914002079234205No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC43621.0822935094644854No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC27170.6741383459915193No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC25680.6371686685705636RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19300.47886897598955913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18820.4669592812499224No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG10520.2610208097103711No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA8600.2133820307518243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7420.18410403118355073No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT6570.1630139467487774No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT6410.15904404850223183No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6030.1496155401666861No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5570.1382020827078676No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5460.13547277766336752No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4560.11314210002654869No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4200.10420982897182117No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4140.10272111712936657No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4050.1004880493656847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10700.034.406541
CGTCTTC2550.031.92156837
TTATACA12500.030.0442
TCGTCTT250.00549216129.614
TTACGAG250.00549216129.635
TATACAC13000.029.4576913
CCGTCTT10750.028.22325737
GGTATCA2650.027.9245281
GCCGTCT11050.027.45701436
GCTCTAC358.8593643E-426.428571
GTATCAA7850.024.0382181
GTCGCCA903.808964E-922.6111112
TCGCCAT957.1104296E-921.42105313
TGCCGTC14650.020.58361835
TTAGGGC450.003821562520.5555553
TCGCTAA450.003821562520.55555525
ATGCCGT15500.019.45483834
GCCGCAG1153.0322553E-919.30434834
CGGCAAG902.145167E-618.518
GTGCTAG801.6124295E-518.51
Produced by FastQC (version 0.11.5)