FastQCFastQC Report
Fri 10 Feb 2017
ERR1631011.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631011.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839343
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT142241.6946587986079589No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT117321.3977599145998716RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG110331.314480492480428No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC55610.6625420120260728No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC50560.6023759059168897RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC33200.39554746986631206RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.21040266017587567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17360.20682843605057763No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13450.16024438161752705No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG13220.15750414312146524No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA11790.14046700812421145No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11730.13975216329915185No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10620.12652753403554926No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT10560.12581268921048963No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10390.12378729553948745No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC10330.12307245071442782No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT9440.11246891914271043No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT9220.10984782145082522No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8910.10615445652135062No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT8630.10281851400440582No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8460.10079312033340364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18800.031.8829771
CGTCTTC6350.030.59055137
AGCTAGC250.005495509629.59999825
TATACAC20600.029.4563083
TTATACA21100.028.6706142
CCGTCTT18400.025.03532637
GATTACG1002.0008883E-1124.051
GCCGTCT19200.023.9921936
GGTATCA3550.023.4507051
AACGCCC400.001930755423.12512
GCGTCTC400.001930755423.1259
GTATCAA9850.022.5380731
TTACGGG1107.4578566E-1121.8636363
TGCCGTC22100.021.01131235
ATGCCGT22500.020.47333334
TTAGAAC1052.257184E-819.380953
TATGCCG23650.019.32135433
ATTACGG1254.110916E-1019.2400022
ATAGCGG801.6158237E-518.57
ACGCCCC500.0070333418.49999813
Produced by FastQC (version 0.11.5)