FastQCFastQC Report
Fri 10 Feb 2017
ERR1631010.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631010.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019574
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT249082.442981088179965No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG191921.8823547873915971No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT153161.5021960151985045Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC100590.9865885163803706No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC58210.5709247195397293Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC50140.49177401542212734No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC32060.31444505254155164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.2390213952101564No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG24080.23617707003120914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23900.23441162681669012No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA17940.17595584038039416No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT15250.1495722723411935No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG14750.144668263411974No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG12440.12201174215898013No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT12230.11995205840870794No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA12080.11848085572994212No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG11660.11436148822939778No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG11380.11161524322903488No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT11320.11102676215752855No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10630.10425922983520568No Hit
TATCAACGCAGAGTACGGGAGCCCTCCAGGACAGGCTGCATCA10350.10151298483484278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC32050.034.2293321
TTATACA34050.032.2187962
GATTACG950.031.1578961
TATACAC35300.031.0779043
CGTCTTC7550.030.38410837
GGTATCA5350.026.9719621
CGCACCT358.868704E-426.42857212
TTACGGG1150.025.739133
CGGATCG951.0913936E-1125.3157937
CCGTCTT23700.025.2911437
GCCGTCT24350.024.61601636
GTATCAA16700.023.9281431
TCGCATG400.001931038523.12537
CCGTTTA502.7018122E-422.227
GGTCGAA450.00382551720.55555530
TCGGACA450.00382551720.55555524
TGCCGTC29600.020.43750235
TTTACCG555.142547E-420.18181830
TTACCGG555.142547E-420.18181831
CGTTTAC555.142547E-420.18181828
Produced by FastQC (version 0.11.5)