FastQCFastQC Report
Fri 10 Feb 2017
ERR1631009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204918
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT139796.821753091480495No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG122025.954576952732312No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT101074.932216789154687RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC62353.042680486828878No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC36941.802672288427566RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC33781.6484642637542821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31551.5396402463424395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28511.3912882226061156No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18560.9057281449165032No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG13600.663680106188817No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA12110.5909680945548951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10930.5333840853414537No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10860.5299680847948937No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT10300.5026400804224128No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10060.4909280785484926No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9860.48116807698689235No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8390.4094320655091305No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8070.3938160630105701RNA PCR Primer, Index 26 (95% over 21bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7910.3860080617612899No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7570.36941605910656944No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7280.3552640568422491No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6990.34111205457792876No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6570.32061605129856824No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5220.2547360407577665No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5040.2459520393523263No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4920.24009603841536614No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4760.23228803716608595No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4720.2303360368537659No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4580.22350403576064573No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4560.2225280356044857No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4470.21813603490176559No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4360.21276803404288547No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4290.20935203349632536No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4100.20008003201280514No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4020.19617603138816503No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3990.194712031153925No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3750.1830000292800047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.17812002849920455No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3620.17665602826496452No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3600.1756800281088045No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3580.17470402795264448No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3540.17275202764032443RNA PCR Primer, Index 26 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3430.1673840267814443No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3300.16104002576640414No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3210.15664802506368403No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3030.1478640236582438No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3020.14737602358016377No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2990.14591202334592374No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2900.14152002264320362No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2780.13566402170624348No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2740.1337120213939234No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2720.1327360212377634No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2610.12736802037888326No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2580.12590402014464322No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2550.12444001991040318No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2520.12297601967616315No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2500.12200001952000313No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2490.12151201944192311No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2470.12053601928576309No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2460.12004801920768307No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2440.11907201905152305No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2430.11858401897344303No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2410.11760801881728301No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2350.11468001834880294No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2340.11419201827072292No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2310.11272801803648289No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2290.11175201788032287No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2280.11126401780224285No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2250.10980001756800281No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2230.10882401741184279No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2220.10833601733376277No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2210.10784801725568277No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2180.10638401702144273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2130.10394401663104266No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2130.10394401663104266No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2120.10345601655296266No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2110.10296801647488264No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10101601616256259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAA200.001838408637.02
AACCTTG200.001838408637.06
CCTTGCG200.001838408637.08
TTTTAAC200.001838408637.02
ATTACGG251.2300878E-437.02
TCGCCGA200.001838408637.01
TCAGAGT308.278665E-637.03
TACGAGC200.001838408637.03
CTTGCGG200.001838408637.09
ACCTTGC200.001838408637.07
TTGCGGC200.001838408637.010
GATTACG251.2300878E-437.01
TTTACAC501.7826096E-1037.02
TTACGGG251.2300878E-437.03
TGCGGCC200.001838408637.011
CGCCGAG200.001838408637.02
CTTATAC16300.035.9785271
CGTCTTC3850.033.63636437
TTATACA17650.033.3314482
ACTCAGA352.3788107E-531.7142871
Produced by FastQC (version 0.11.5)