FastQCFastQC Report
Fri 10 Feb 2017
ERR1631008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372930
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG132883.563135172820637No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT112803.024696323706862TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC73511.9711474003164131No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT55721.494114176923283No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC43711.172069825436409RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC35280.9460220416700185No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG17740.4756924892070898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.2863808221382029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10210.27377792078942426No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT10170.27270533344059206No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA9880.2649290751615585No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9770.2619794599522699No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5190.1391682085109806No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4760.12763789451103424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4540.12173866409245704No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT4240.11369425897621538TruSeq Adapter, Index 12 (95% over 21bp)
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4010.10752688172043011No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3870.10377282599951733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7350.033.224491
CGTCTTC6650.031.71428737
CGGAGAT303.594857E-430.8333341
TAATACG250.00549164129.62
TGAACGC250.00549164129.64
TTATACA8950.027.4916212
TATACAC9900.025.6010073
GTATCAA4650.025.4623641
CCGTCTT18550.023.7358537
GCCGTCT19050.023.1128636
GGTATCA1700.022.8529431
TACACAT26000.021.2752
ATACACA26050.021.0211121
CTATGCA450.00382103520.5555559
TAAGAGT450.00382103520.5555554
CTTATAA555.1341444E-420.1818181
TGCCGTC22650.019.68432635
ATGCCGT23250.019.25591534
GATACAC801.6119024E-518.53
CGTATAC500.007026188518.51
Produced by FastQC (version 0.11.5)