##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631008.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372930
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.145413348349557	31.0	30.0	31.0	26.0	34.0
2	30.140047730137024	31.0	30.0	33.0	26.0	34.0
3	29.585477167296812	31.0	30.0	33.0	25.0	34.0
4	33.813742525406916	35.0	33.0	37.0	28.0	37.0
5	33.40635239857346	35.0	33.0	37.0	28.0	37.0
6	33.713683533102724	35.0	33.0	37.0	29.0	37.0
7	33.81142841820181	35.0	33.0	37.0	29.0	37.0
8	33.88403990024938	35.0	33.0	37.0	29.0	37.0
9	35.470270560158745	37.0	34.0	39.0	30.0	39.0
10	35.0967634676749	37.0	34.0	39.0	29.0	39.0
11	35.28963880621028	37.0	34.0	39.0	30.0	39.0
12	35.16412999758668	37.0	34.0	39.0	30.0	39.0
13	35.358198589547634	37.0	34.0	39.0	30.0	39.0
14	36.105561365403695	38.0	34.0	40.0	30.0	41.0
15	36.123218834633846	38.0	34.0	40.0	30.0	41.0
16	36.115742900812485	38.0	34.0	40.0	30.0	41.0
17	36.01665996299574	38.0	34.0	40.0	29.0	41.0
18	36.11278524119808	38.0	34.0	40.0	30.0	41.0
19	36.09573914675676	38.0	34.0	40.0	29.0	41.0
20	36.12548467540825	38.0	34.0	40.0	30.0	41.0
21	36.00738744536508	38.0	34.0	40.0	29.0	41.0
22	35.75178988013836	38.0	34.0	40.0	29.0	41.0
23	35.821449065508276	38.0	34.0	40.0	29.0	41.0
24	35.63388303434961	38.0	34.0	40.0	28.0	41.0
25	35.55079237390395	38.0	34.0	40.0	27.0	41.0
26	35.36215107392808	38.0	34.0	40.0	27.0	41.0
27	35.19552194781863	38.0	33.0	40.0	27.0	40.0
28	35.171431635963856	37.0	33.0	40.0	27.0	40.0
29	35.002169307913015	37.0	33.0	39.0	27.0	40.0
30	34.883506288043336	37.0	33.0	39.0	27.0	40.0
31	34.88737296543587	37.0	33.0	39.0	27.0	40.0
32	34.533386962700774	37.0	33.0	39.0	25.0	40.0
33	34.4737511061057	37.0	33.0	39.0	25.0	40.0
34	34.45642077601695	37.0	33.0	39.0	25.0	40.0
35	34.35365081918859	37.0	32.0	39.0	25.0	40.0
36	34.506231732496715	37.0	33.0	39.0	25.0	40.0
37	34.51255999785482	37.0	33.0	39.0	25.0	40.0
38	34.39078647467353	37.0	32.0	39.0	25.0	40.0
39	34.23434692837798	37.0	32.0	39.0	25.0	40.0
40	33.94363285335049	37.0	32.0	39.0	24.0	40.0
41	33.98797361435122	37.0	32.0	39.0	24.0	40.0
42	33.9472340653742	37.0	32.0	39.0	24.0	40.0
43	32.99300136754887	36.0	30.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	16.0
11	19.0
12	16.0
13	12.0
14	16.0
15	22.0
16	26.0
17	37.0
18	62.0
19	115.0
20	203.0
21	427.0
22	763.0
23	1253.0
24	1980.0
25	3104.0
26	4533.0
27	6579.0
28	9173.0
29	11951.0
30	15652.0
31	19956.0
32	25384.0
33	31364.0
34	39289.0
35	46639.0
36	51667.0
37	50304.0
38	38727.0
39	13631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.20515914514788	26.504705976993005	17.20832327782694	23.08181160003218
2	13.95838361086531	23.09495079505537	39.55782586544392	23.3888397286354
3	15.749872630252327	30.485345775346577	34.513179417048775	19.251602177352318
4	11.92609873166546	19.73533907167565	33.71973292575014	34.618829270908755
5	11.867106427479687	40.37781889362615	33.44488241761188	14.310192261282278
6	26.532593248062643	38.54503526130909	17.805754431126488	17.11661705950178
7	21.816962968921782	36.34649934304025	21.30319362883115	20.533344059206822
8	24.811358700024133	33.55267744616952	24.200520204864183	17.43544364894216
9	21.569999731853162	15.147614833883033	18.165875633496903	45.1165098007669
10	13.685678277424717	27.81084922103344	33.53337087389055	24.9701016276513
11	29.42804279623522	22.577695546081035	23.77872522993591	24.215536427747836
12	18.18008741586893	28.667042072238758	27.026519722199875	26.126350789692438
13	30.55881800874159	19.660526104094604	25.24334325476631	24.5373126323975
14	21.58153004585311	25.310111817231117	24.440511624165392	28.667846512750383
15	25.174697664441048	29.70289330437347	21.041750462553292	24.08065856863218
16	25.101761724720458	27.551551229453246	21.528168825248706	25.81851822057759
17	19.310862628375297	33.21373984393854	22.830826160405437	24.644571367280722
18	23.054460622636956	24.71965248169898	23.947657737376986	28.278229158287076
19	26.064408870297374	29.029576596144047	23.96347840077226	20.942536132786312
20	21.834660660177512	25.643954629555143	24.26621617998016	28.255168530287182
21	29.382994127584265	24.903333065186494	24.545357037513742	21.168315769715495
22	19.028772155632424	33.581369157750785	26.997828010618612	20.39203067599818
23	25.53642774783472	27.766873139731317	23.95731102351648	22.739388088917494
24	27.00533612206044	28.74105059930818	25.60882739388089	18.64478588475049
25	20.361730083393667	28.16882524870619	27.304051698710214	24.165392969189927
26	28.050036199823026	28.85662188614485	24.178264017375913	18.91507789665621
27	19.288338294049822	29.411685839165525	28.2490011530314	23.05097471375325
28	22.902689512777197	27.420963719732928	27.733891078754723	21.94245568873515
29	23.225002011101278	25.249778778859305	29.426702062049177	22.09851714799024
30	18.805673987075323	29.043520231678865	30.506261228648807	21.644544552597004
31	26.566379749550855	26.36553776848202	24.53463116402542	22.533451317941704
32	21.997425790362804	26.492103075644224	32.60451022980184	18.905960904191137
33	19.170085538841068	30.800686455903254	30.092778805673987	19.93644919958169
34	21.36084519883088	31.205856326924625	26.2151073928083	21.218191081436196
35	20.404633577346956	24.730914648861717	35.9753841203443	18.88906765344703
36	22.667524736545733	27.350709248384415	24.01335371249296	25.968412302576894
37	21.818839996782238	25.394309924114445	29.282707210468452	23.504142868634865
38	21.76199286729413	23.982248679376827	28.06558871638109	26.190169736947954
39	26.51275038210924	25.359718982114604	26.338991231598424	21.78853940417773
40	19.898640495535354	23.29096613305446	34.96849274662806	21.84190062478213
41	22.365323251012253	27.220658032338505	25.74826374922908	24.66575496742016
42	21.180650524227065	23.50494730914649	33.404928538867885	21.90947362775856
43	23.20194138310139	23.526935349797547	29.78065588716381	23.490467379937254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	32.5
4	56.0
5	56.0
6	84.5
7	113.0
8	114.0
9	115.0
10	191.0
11	267.0
12	267.0
13	374.0
14	481.0
15	1299.5
16	2118.0
17	2325.0
18	2532.0
19	2532.0
20	2717.0
21	2902.0
22	3584.5
23	4267.0
24	5393.5
25	6520.0
26	6520.0
27	7963.0
28	9406.0
29	11680.0
30	13954.0
31	15055.0
32	16156.0
33	16156.0
34	17454.0
35	18752.0
36	19579.5
37	20407.0
38	22159.0
39	23911.0
40	23911.0
41	24899.5
42	25888.0
43	24352.5
44	22817.0
45	22868.0
46	22919.0
47	22919.0
48	24095.5
49	25272.0
50	34343.5
51	43415.0
52	34003.0
53	24591.0
54	24591.0
55	30948.5
56	37306.0
57	30440.0
58	23574.0
59	16196.5
60	8819.0
61	8819.0
62	7435.5
63	6052.0
64	5108.0
65	4164.0
66	3312.5
67	2461.0
68	2461.0
69	1988.5
70	1516.0
71	1233.0
72	950.0
73	761.0
74	572.0
75	572.0
76	429.0
77	286.0
78	240.0
79	194.0
80	138.5
81	83.0
82	83.0
83	64.5
84	46.0
85	33.0
86	20.0
87	15.0
88	10.0
89	10.0
90	8.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	372930.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.795422455329714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.66288528127093	48.91187717921617
2	7.392315684873256	8.249147531213266
3	1.8326700753262841	3.0676380322221286
4	0.7843958460074175	1.7506279040075845
5	0.4527142661900978	1.2629691866815551
6	0.3009784035479572	1.0075930305533387
7	0.2092619924873054	0.8173102892271267
8	0.1562127711655842	0.6972766048081215
9	0.1439422880933192	0.722818869601817
>10	0.8926535782948886	9.733172301649514
>50	0.11288521227542322	4.3395174061477
>100	0.05283988659700545	5.300906383718597
>500	0.0014410878162819667	0.665762537018758
>1k	0.0028821756325639334	3.425031988954391
>5k	9.607252108546445E-4	3.463626918636638
>10k+	9.607252108546445E-4	6.584723836343335
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	13288	3.563135172820637	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	11280	3.024696323706862	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	7351	1.9711474003164131	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	5572	1.494114176923283	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	4371	1.172069825436409	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3528	0.9460220416700185	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1774	0.4756924892070898	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.2863808221382029	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1021	0.27377792078942426	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	1017	0.27270533344059206	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	988	0.2649290751615585	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	977	0.2619794599522699	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	519	0.1391682085109806	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	476	0.12763789451103424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	454	0.12173866409245704	No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT	424	0.11369425897621538	TruSeq Adapter, Index 12 (95% over 21bp)
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	401	0.10752688172043011	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	387	0.10377282599951733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.6814683720805514E-4	0.0
11	0.0	0.0	0.0	2.6814683720805514E-4	0.0
12	0.0	0.0	0.0	2.6814683720805514E-4	0.0
13	0.0	0.0	0.0	2.6814683720805514E-4	0.0
14	0.0	0.0	0.0	2.6814683720805514E-4	0.0
15	0.0	0.0	0.0	2.6814683720805514E-4	0.0
16	0.0	0.0	0.0	2.6814683720805514E-4	0.0
17	0.0	0.0	0.0	2.6814683720805514E-4	0.0
18	0.0	0.0	0.0	5.362936744161103E-4	0.0
19	0.0	0.0	0.0	5.362936744161103E-4	0.0
20	0.0	0.0	0.0	5.362936744161103E-4	0.0
21	0.0	0.0	0.0	8.044405116241654E-4	0.0
22	0.0	0.0	0.0	0.0013407341860402757	0.0
23	0.0	0.0	0.0	0.0016088810232483307	0.0
24	0.0	0.0	0.0	0.0016088810232483307	0.0
25	0.0	0.0	0.0	0.001877027860456386	0.0
26	0.0	0.0	0.0	0.002145174697664441	0.0
27	0.0	0.0	0.0	0.0026814683720805515	0.0
28	0.0	0.0	0.0	0.004290349395328882	0.0
29	0.0	0.0	0.0	0.006971817767409434	0.0
30	0.0	0.0	0.0	0.013407341860402756	0.0
31	0.0	0.0	0.0	0.036736116697503554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	735	0.0	33.22449	1
CGTCTTC	665	0.0	31.714287	37
CGGAGAT	30	3.594857E-4	30.833334	1
TAATACG	25	0.005491641	29.6	2
TGAACGC	25	0.005491641	29.6	4
TTATACA	895	0.0	27.491621	2
TATACAC	990	0.0	25.601007	3
GTATCAA	465	0.0	25.462364	1
CCGTCTT	1855	0.0	23.73585	37
GCCGTCT	1905	0.0	23.11286	36
GGTATCA	170	0.0	22.852943	1
TACACAT	2600	0.0	21.275	2
ATACACA	2605	0.0	21.021112	1
CTATGCA	45	0.003821035	20.555555	9
TAAGAGT	45	0.003821035	20.555555	4
CTTATAA	55	5.1341444E-4	20.181818	1
TGCCGTC	2265	0.0	19.684326	35
ATGCCGT	2325	0.0	19.255915	34
GATACAC	80	1.6119024E-5	18.5	3
CGTATAC	50	0.0070261885	18.5	1
>>END_MODULE