FastQCFastQC Report
Fri 10 Feb 2017
ERR1631007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322437
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT189915.889832742520245No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG140544.358680920613949No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT131804.087620217282756RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC70282.179650598411473No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC57901.795699625043032RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56321.7466978045323582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49041.520917264457863No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC40651.2607113947840973No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31160.966390333615559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20170.6255485567723308No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17250.5349882302589343No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA16540.512968424839581No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16120.4999426244506679No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG16000.4962209671966927No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13830.42892099852064125No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12680.39325511650337897No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12650.39232470218988513No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT12510.3879827687269141No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12390.3842611114729389No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11640.3610007536355939No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11050.34270260547021586No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10990.3408417768432283No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8970.2781938797346458No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8870.2750924986896665No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8670.26888973659970783No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8060.24997131222533395No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8040.2493510360163381No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7220.22391971144750758No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7180.22267915902951582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.22205888282051997No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7050.2186473636710427No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7050.2186473636710427No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6890.21368515399907578No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6740.20903308243160681No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6640.20593170138662747No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6210.19259576289321637No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5910.18329161975827837No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5850.18143079113129076No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5680.1761584433548259No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5680.1761584433548259No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5560.1724367861008507No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5370.16654416211538997No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5320.16499347159290031No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5170.16034140002543135No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4980.15444877603997062No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4910.15227780930848506No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4910.15227780930848506No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4760.1476257377410161No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4560.1414229756510574No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4430.1373911802925843No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4430.1373911802925843No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4380.13584048977009464No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4230.1311884182026256No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4190.1299478657846339No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4130.1280870371576463No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT4090.12684648473965457No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4030.12498565611266697No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3990.12374510369467524No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3960.12281468938118144No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3930.12188427506768765No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3850.11940317023170417No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.11878289402270832No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3830.11878289402270832No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3830.11878289402270832No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3780.11723220350021864No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3680.11413082245523931No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3680.11413082245523931No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3600.11164971761925585No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3530.10947875088777033No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3530.10947875088777033No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3530.10947875088777033No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3490.10823819846977858No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3480.10792806036528065No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3460.10730778415628481No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3380.10482667932030132No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3360.10420640311130547No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3310.1026557125888158No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3290.10203543637981993No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3280.101725298275322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG200.001839972537.01
TCGCCGA452.6084308E-937.01
GATACAC308.293433E-637.03
ATTACTT200.001839972537.02
ATTACGG452.6084308E-937.02
TTACTTT200.001839972537.03
TCTACAC200.001839972537.03
TTACGGG452.6084308E-937.03
GATTACG452.6084308E-937.01
CTTATAC19200.035.9401051
TTATACA20650.033.5060542
CGTCTTC7300.033.1986337
ACTCAGA451.07587766E-732.888891
TATACAC21950.031.5216393
TAGGACC601.2641976E-930.8333344
CGCCGAG551.8470018E-830.2727282
CTCAGAG551.8470018E-830.2727282
AGCAACG250.005490552629.5999982
ATTAGCG250.005490552629.5999982
TTAGCGG250.005490552629.5999983
Produced by FastQC (version 0.11.5)