FastQCFastQC Report
Fri 10 Feb 2017
ERR1631006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728348
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT183582.520498443051948No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG154702.1239846886378486No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT144151.9791363469110919TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC84221.1563153876992867No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC55440.7611746033489486RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC40510.5561901728294716TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG19610.2692394295034791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.267179974407838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19170.2631983612229319No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA15060.2067692916023659No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13060.1793098903271513No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT11260.15459642917945818No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10340.14196510459285946No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7970.10942571408173016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20750.033.8795171
GATTACG352.3871162E-531.7142851
CGTCTTC7350.030.70748337
TATACAC23950.030.2025053
TTATACA23500.030.1510642
GGTATCA3900.027.5128171
CCGTCTT22650.025.40176637
GCCGTCT23200.024.71982836
GTATCAA9550.022.664921
TTAGTAC603.7238744E-521.5833323
GCTCTAG957.1340764E-921.4210531
TGCCGTC27450.020.82513835
TTCGCAC450.003824482220.55555527
CCTTAGT555.1406084E-420.1818181
TAGCACT555.1406084E-420.1818184
TTTGGCG555.1406084E-420.18181822
ATGCCGT28250.020.16991234
TCGCCGA656.897695E-519.9230771
TCTATTC1153.0449883E-919.3043483
TATGCCG29900.018.80936633
Produced by FastQC (version 0.11.5)