FastQCFastQC Report
Fri 10 Feb 2017
ERR1631005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583688
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG128772.206144378503584No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT120712.06805690711476TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT112961.9352804923178135No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC70991.2162319595400282No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC44130.7560546045147407RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC32180.5513219391181591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.3203766395745672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18310.3136949877331725No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG15910.27257713024766655No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA11880.20353339455325448No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT10240.17543619193815874No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9660.16549937637916146No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9150.15676183166349145No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7820.13397568564027357No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7270.12455284329984512No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT7070.12112635517605295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6970.11941311111415688No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA6920.11855648908320883No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC6800.11650059620893355No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6660.11410205452227903No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6450.11050424199229726No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT6190.10604980743136744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14450.033.543251
CGTCTTC5850.031.9401737
TTATACA16150.030.4705892
TATACAC17350.028.7896233
TTACGGG454.003812E-628.7777793
GATTACG454.003812E-628.7777791
CGGGATA405.9351976E-527.7517
GGTATCA3850.027.389611
CCGTCTT18000.024.97537
GCCGTCT18650.024.30294836
GTATCAA10400.023.3028851
CCGAGGT555.138927E-420.18181832
TGCCGTC22750.020.08571435
CTTACAC759.252966E-619.7333323
ATTGGTC851.2431592E-619.58823616
ATACAGT1254.110916E-1019.246
ATGCCGT23700.019.20253234
GTCCTAG609.2293904E-418.51
CGGGGTG902.1485248E-618.516
ACAGCGT500.00703083518.58
Produced by FastQC (version 0.11.5)