##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631005.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583688
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97543036690835	31.0	30.0	34.0	28.0	34.0
2	30.988766258686148	31.0	31.0	34.0	27.0	34.0
3	30.405466619152698	31.0	30.0	34.0	26.0	34.0
4	34.623362138676825	35.0	35.0	37.0	32.0	37.0
5	34.741123682515315	35.0	35.0	37.0	32.0	37.0
6	34.95299886240594	35.0	35.0	37.0	32.0	37.0
7	34.97564280917202	36.0	35.0	37.0	32.0	37.0
8	35.01583722810817	36.0	35.0	37.0	32.0	37.0
9	36.7198212058497	38.0	35.0	39.0	32.0	39.0
10	36.498178821562206	38.0	35.0	39.0	32.0	39.0
11	36.572273200751084	38.0	35.0	39.0	32.0	39.0
12	36.48202464330259	38.0	35.0	39.0	32.0	39.0
13	36.59260598127767	38.0	35.0	39.0	32.0	39.0
14	37.55054069982594	39.0	36.0	40.0	32.0	41.0
15	37.55761982428969	39.0	36.0	40.0	32.0	41.0
16	37.546242170474635	39.0	36.0	40.0	32.0	41.0
17	37.51984279272488	39.0	36.0	40.0	32.0	41.0
18	37.57245823110977	39.0	37.0	40.0	32.0	41.0
19	37.58770267677252	39.0	37.0	40.0	32.0	41.0
20	37.57422629898165	39.0	37.0	40.0	32.0	41.0
21	37.509996779101165	39.0	37.0	40.0	32.0	41.0
22	37.4121345650416	39.0	36.0	40.0	32.0	41.0
23	37.45082818217952	39.0	36.0	40.0	32.0	41.0
24	37.36610826331876	39.0	36.0	40.0	32.0	41.0
25	37.32702231329066	39.0	36.0	40.0	32.0	41.0
26	37.17124902345088	39.0	36.0	40.0	31.0	41.0
27	37.04219034826825	39.0	36.0	40.0	31.0	41.0
28	37.052988925590384	39.0	36.0	40.0	31.0	41.0
29	36.97732178835268	39.0	36.0	40.0	31.0	41.0
30	36.89220097038144	39.0	36.0	40.0	31.0	41.0
31	36.86311865243075	39.0	36.0	40.0	31.0	41.0
32	36.74874076561451	39.0	35.0	40.0	30.0	41.0
33	36.70934985814339	39.0	35.0	40.0	30.0	41.0
34	36.64661600032894	38.0	35.0	40.0	30.0	41.0
35	36.643191225449215	38.0	35.0	40.0	30.0	41.0
36	36.64100341278217	38.0	35.0	40.0	30.0	41.0
37	36.57494757473171	38.0	35.0	40.0	30.0	41.0
38	36.47815613821083	38.0	35.0	40.0	30.0	41.0
39	36.41770260824276	38.0	35.0	40.0	30.0	41.0
40	36.26569331560697	38.0	35.0	40.0	30.0	41.0
41	36.265607653403876	38.0	35.0	40.0	30.0	41.0
42	36.21430284672633	38.0	35.0	40.0	30.0	41.0
43	35.42213819711901	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	18.0
11	16.0
12	17.0
13	14.0
14	7.0
15	22.0
16	19.0
17	16.0
18	31.0
19	54.0
20	92.0
21	187.0
22	373.0
23	720.0
24	1256.0
25	2016.0
26	3262.0
27	4830.0
28	7099.0
29	10086.0
30	13486.0
31	17313.0
32	22324.0
33	29125.0
34	37780.0
35	51087.0
36	69814.0
37	99967.0
38	141539.0
39	71109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.74495278299366	24.258850618823754	16.402769972999273	23.593426625183316
2	13.635538164224723	22.85656035416181	40.98987815408231	22.518023327531147
3	15.013157714395362	29.398240155699618	35.25736352297803	20.331238606926988
4	11.554117953427173	19.92845492797522	36.519167774564494	31.998259344033116
5	11.762277106947547	39.65697427392716	35.13383862611532	13.446909993009964
6	26.711873466646562	40.14370691191184	17.88712462822604	15.257294993215552
7	22.592549444223625	35.000034264881236	22.456003892490507	19.951412398404628
8	24.306307479338276	35.4490755335042	23.008182453639616	17.236434533517908
9	22.544407286084347	15.08442866737024	19.29472594947986	43.076438097065555
10	13.471580707501268	29.089170926933566	33.916750044544344	23.52249832102082
11	29.830320308109815	23.182419374734447	25.39712997354751	21.59013034360823
12	18.870698044160577	27.80749304422911	28.914934005838738	24.406874905771577
13	29.071353188689848	20.64990200243966	26.85338742615918	23.42535738271131
14	21.158735488822796	23.85572429105961	26.35055029399268	28.634989926124916
15	23.892387713984185	29.679897479475336	22.838571291511904	23.589143515028578
16	24.623086306382863	27.97042255451543	23.112690341415277	24.293800797686433
17	19.92794095475665	32.12041364564631	24.517036498951494	23.43460890064555
18	22.34087389153109	25.62105097243733	25.75554063129617	26.282534504735406
19	23.397088855690026	27.813146749633365	25.90065240333877	22.88911199133784
20	23.46167815682351	25.849083757075697	26.724380148298405	23.964857937802385
21	23.27202203917161	26.722666904236508	27.20357451241074	22.801736544181136
22	19.762098929565113	33.78311700771645	25.431737503597812	21.023046559120626
23	21.94802702813832	25.546010882526282	27.090500404325596	25.4154616850098
24	22.662449801948988	29.29835802689108	27.386548978221242	20.652643192938694
25	22.53498444374392	29.64272008333219	25.779183399350337	22.043112073573553
26	24.14235002261482	26.28373377557873	27.081420210797553	22.492495991008894
27	21.933978426830773	31.13444168802511	26.48349803319582	20.448081851948302
28	19.546058853360016	25.77747015528844	30.61824125217582	24.058229739175722
29	20.43540384589027	28.620598676004988	27.23835336686723	23.70564411123751
30	21.710228752347145	27.399055659873085	28.0812694453201	22.80944614245967
31	22.42516549937638	25.408266059949835	30.161490385274327	22.00507805539946
32	21.171584819287016	31.206569263030936	27.416188100492043	20.205657817190005
33	21.77807321719823	25.842230780828114	29.865955784597247	22.51374021737641
34	19.94096160962706	29.729410232864133	28.73795589424487	21.591672263263938
35	20.595420841271363	28.01016981675141	31.65475390962295	19.739655432354272
36	24.583167719740683	26.310117734131932	25.06664519400776	24.040069352119627
37	21.880696536505802	26.908725209358426	28.211647318430394	22.998930935705378
38	21.44861638409561	25.500438590479845	28.793807650662686	24.257137374761857
39	24.436514028042378	25.86981401022464	27.067885582708573	22.625786379024408
40	20.176361343731582	24.94174970189553	33.30854840257123	21.57334055180165
41	21.80462850015762	27.121681446252104	26.711188169021803	24.36250188456847
42	22.017242088238923	24.83895505818177	31.349796466605444	21.794006386973862
43	22.230712298351175	24.879730266854896	30.133735831471608	22.755821603322325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	13.0
2	17.0
3	61.0
4	105.0
5	105.0
6	164.0
7	223.0
8	223.5
9	224.0
10	337.0
11	450.0
12	450.0
13	697.5
14	945.0
15	2334.0
16	3723.0
17	4185.5
18	4648.0
19	4648.0
20	4925.0
21	5202.0
22	6476.5
23	7751.0
24	9582.5
25	11414.0
26	11414.0
27	13915.0
28	16416.0
29	20562.5
30	24709.0
31	25910.0
32	27111.0
33	27111.0
34	29471.0
35	31831.0
36	32994.0
37	34157.0
38	36211.5
39	38266.0
40	38266.0
41	40116.0
42	41966.0
43	39174.5
44	36383.0
45	37227.5
46	38072.0
47	38072.0
48	39183.5
49	40295.0
50	51339.5
51	62384.0
52	50173.0
53	37962.0
54	37962.0
55	43052.5
56	48143.0
57	39183.5
58	30224.0
59	22234.0
60	14244.0
61	14244.0
62	12187.5
63	10131.0
64	8359.5
65	6588.0
66	5374.5
67	4161.0
68	4161.0
69	3325.5
70	2490.0
71	2047.0
72	1604.0
73	1259.5
74	915.0
75	915.0
76	678.5
77	442.0
78	356.0
79	270.0
80	192.0
81	114.0
82	114.0
83	87.0
84	60.0
85	41.0
86	22.0
87	17.5
88	13.0
89	13.0
90	8.5
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	583688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.85346323195955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.93234348177526	44.88968493418169
2	9.222324244954843	9.748635507878598
3	2.2234230228209726	3.5254682095728187
4	0.9345726235295001	1.9758159918124962
5	0.5413088897257491	1.430502475012636
6	0.3442055113551111	1.091547200318712
7	0.2377368508683877	0.8795651134377926
8	0.18861555181326964	0.7975188106190726
9	0.1384675163259112	0.6586639004657076
>10	1.0194707603418987	10.710307521455485
>50	0.12551147571527793	4.626759229926225
>100	0.08324077073534951	7.762822711264178
>500	0.0052025481709593435	1.8528003464719747
>1k	0.0022761148247947127	2.601069774960888
>5k	3.2515926068495897E-4	1.220019447138906
>10k+	9.754777820548769E-4	6.228818825482817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	12877	2.206144378503584	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	12071	2.06805690711476	TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	11296	1.9352804923178135	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	7099	1.2162319595400282	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	4413	0.7560546045147407	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3218	0.5513219391181591	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1870	0.3203766395745672	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1831	0.3136949877331725	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1591	0.27257713024766655	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1188	0.20353339455325448	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	1024	0.17543619193815874	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	966	0.16549937637916146	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	915	0.15676183166349145	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	782	0.13397568564027357	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	727	0.12455284329984512	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	707	0.12112635517605295	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	697	0.11941311111415688	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	692	0.11855648908320883	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	680	0.11650059620893355	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	666	0.11410205452227903	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	645	0.11050424199229726	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	619	0.10604980743136744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.426488123792163E-4	0.0
16	0.0	0.0	0.0	5.139732185688244E-4	0.0
17	0.0	0.0	0.0	6.852976247584326E-4	0.0
18	0.0	0.0	0.0	6.852976247584326E-4	0.0
19	0.0	0.0	0.0	6.852976247584326E-4	0.0
20	0.0	0.0	0.0	8.566220309480407E-4	0.0
21	0.0	0.0	0.0	0.001199270843327257	0.0
22	0.0	0.0	0.0	0.001199270843327257	0.0
23	0.0	0.0	0.0	0.0018845684680856897	0.0
24	0.0	0.0	0.0	0.0022272172804649057	0.0
25	0.0	0.0	0.0	0.002398541686654514	0.0
26	0.0	0.0	0.0	0.0030838393114129468	0.0
27	0.0	0.0	0.0	0.0035978125299817713	0.0
28	0.0	0.0	0.0	0.00925151793423884	0.0
29	0.0	0.0	0.0	0.020558928742752978	0.0
30	0.0	0.0	0.0	0.032037663957456726	0.0
31	0.0	0.0	0.0	0.06750181603870561	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1445	0.0	33.54325	1
CGTCTTC	585	0.0	31.94017	37
TTATACA	1615	0.0	30.470589	2
TATACAC	1735	0.0	28.789623	3
TTACGGG	45	4.003812E-6	28.777779	3
GATTACG	45	4.003812E-6	28.777779	1
CGGGATA	40	5.9351976E-5	27.75	17
GGTATCA	385	0.0	27.38961	1
CCGTCTT	1800	0.0	24.975	37
GCCGTCT	1865	0.0	24.302948	36
GTATCAA	1040	0.0	23.302885	1
CCGAGGT	55	5.138927E-4	20.181818	32
TGCCGTC	2275	0.0	20.085714	35
CTTACAC	75	9.252966E-6	19.733332	3
ATTGGTC	85	1.2431592E-6	19.588236	16
ATACAGT	125	4.110916E-10	19.24	6
ATGCCGT	2370	0.0	19.202532	34
GTCCTAG	60	9.2293904E-4	18.5	1
CGGGGTG	90	2.1485248E-6	18.5	16
ACAGCGT	50	0.007030835	18.5	8
>>END_MODULE