FastQCFastQC Report
Fri 10 Feb 2017
ERR1631004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594717
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG86251.4502696240396693No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT82141.3811611236941268No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT61491.0339371499385421No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC55770.9377569499442592No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC29230.49149427374700905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.42137689018474334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24120.4055710531227458No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC19100.3211611573235673Illumina PCR Primer Index 7 (95% over 21bp)
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG14060.2364149671188145No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8890.14948286327782792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8230.1383851478938722No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8000.1345177622297664No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA7930.13334073181025596No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT7340.12342004684581069No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC6880.11568527551759913No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT6740.11333121467857821No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA6720.1129949202730038No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA6450.10845494579774918No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6240.1049238545392178No Hit
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT6040.10156091048347365RNA PCR Primer, Index 41 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10150.032.6256141
ATTACGG551.8526407E-830.2727282
GCGTTTA250.00549423829.59999825
TTATACA11400.029.2105262
TATACAC12300.027.8252053
CGTCTTC2600.027.7537
GATTACG604.3153705E-827.7499981
TTACGGG556.2532126E-726.909093
CCGTCTT8750.026.64000137
GCCGTCT8900.025.98314736
GTATCAA9000.025.488891
TCGCCAT1100.025.22727213
GTCGCCA1103.6379788E-1223.54545412
GGTATCA3050.023.049181
TCGCACC450.003823669220.55555728
ACGCTTT450.003823669220.55555722
TAGGACC450.003823669220.5555574
CCGTACA1001.2865712E-820.34999817
TTATACT1202.382876E-1020.0416664
TGCCGTC11600.019.93534535
Produced by FastQC (version 0.11.5)