FastQCFastQC Report
Fri 10 Feb 2017
ERR1631003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411564
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT233975.684899553896843No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG184904.4926184019982305No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT163603.9750804249156877TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC93222.2650183203584375No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC67051.6291512377175847RNA PCR Primer, Index 1 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53991.311825135337396No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC50221.2202233431495466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49781.2095324178013627No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA28860.7012275126104324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20430.4963991019622707No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG19900.483521396429231No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA19740.4796337872117095No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17800.4324965254492618No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16410.39872292037204415No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT15760.3829295079258633No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15250.37053775354501367No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13550.3292319056088482No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12880.3129525420104771No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12740.30955088394514585No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT11600.2818516682703055No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10810.2626565977587933No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10800.2624136221826982No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10570.2568251839325111No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8340.20264163046330586No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8280.20118377700673531No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8210.19948294797406965No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7640.18563334013664948No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7600.1846614378322691No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7460.18125977976693783No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7430.18053085303865254No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7310.17761514612551146No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7200.17494241478846548No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6870.16692422077732746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.1659523184729471No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6810.16546636732075692No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6780.16473744059247164No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6260.1521027106355269No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6170.1499159304506711No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6140.14918700372238583No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5770.14019690740686747No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5760.13995393183077237No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5650.13728120049372636No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT5390.130963835515254RNA PCR Primer, Index 40 (95% over 22bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5290.1285340797543031No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5230.12707622629773258No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5120.12440349496068656No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4910.11930100786268964No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4880.11857208113440437No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4810.11687125210173874No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4740.1151704230690731No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4630.11249769173202709No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.11055388712326639No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4540.11031091154717128No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4500.10933900924279091No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4430.10763818021012528No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4380.10642330232964983No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4360.10593735117745964No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4310.1047224732969842No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4300.1044794977208891No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4120.10010593735117745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT200.001840563337.01
ATTACTT452.6120688E-937.02
GATTACG600.037.01
ATACATC200.001840563337.03
CTTATAC27000.035.492591
TTACGGG651.8189894E-1234.1538473
TTATACA28400.033.6778142
CGTCTTC8350.033.23353637
TATACAC30050.032.3211333
TCGATCA303.5954994E-430.83333434
AATGATC303.5954994E-430.8333347
CGCCGAG303.5954994E-430.8333342
TTCGATC303.5954994E-430.83333433
AACCAGG250.005492295629.628
TGGGGCG250.005492295629.637
ACAGAGT250.005492295629.63
AGTGTTA250.005492295629.612
AGACAGA454.0000414E-628.77777715
GTATACA454.0000414E-628.7777772
CAGACAC454.0000414E-628.77777718
Produced by FastQC (version 0.11.5)