FastQCFastQC Report
Fri 10 Feb 2017
ERR1631002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350914
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG96762.7573707518081356No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT80802.302558461617376RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC58991.6810386590446664No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC32040.9130442216611477RNA PCR Primer, Index 29 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.8534854693742625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27050.7708441384498766No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC26630.7588753939711724RNA PCR Primer, Index 29 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT25980.7403523370398445No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG14480.4126367144086585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10520.2997885521808762No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT7680.2188570418963051No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT7020.20004901485834137No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA6960.19833919421852647No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6160.17554158568766137No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3990.11370307254768976No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3760.10714876009506602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.1023042682822572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG251.2318786E-437.02
CGTCTTC2900.032.5344837
CTTATAC3800.031.1578961
GCAACGC303.5944284E-430.8333343
GGTATCA2750.030.2727261
CTTCGGT250.00549120629.62
GCACGTG405.928555E-527.757
CCGTCTT11550.027.3896137
GTATCAA11150.027.2107621
GCCGTCT11950.026.47280336
ACGTGTG451.3206541E-424.6666689
AGTAGTG451.3206541E-424.6666685
TAGGACC451.3206541E-424.6666684
GACTATT451.3206541E-424.6666682
TTATACA4850.024.412372
ATACACA17350.022.8184431
TACACAT17800.022.1376422
CGTGTGT806.931805E-720.812510
ATCACGC450.003820591620.55555728
GGATTCG450.003820591620.55555733
Produced by FastQC (version 0.11.5)