##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631002.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350914
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.518446115002536	31.0	30.0	31.0	25.0	34.0
2	29.445260092216326	31.0	30.0	31.0	25.0	34.0
3	28.902246704320717	31.0	28.0	31.0	22.0	34.0
4	33.037165801307445	35.0	32.0	37.0	26.0	37.0
5	32.22170104356053	35.0	32.0	35.0	25.0	37.0
6	32.631337022746315	35.0	32.0	36.0	26.0	37.0
7	32.805288475238946	35.0	32.0	36.0	26.0	37.0
8	32.9101004804596	35.0	32.0	36.0	26.0	37.0
9	34.319707962634716	37.0	32.0	39.0	27.0	39.0
10	33.87492946989861	35.0	32.0	39.0	27.0	39.0
11	34.17917495454727	37.0	32.0	39.0	27.0	39.0
12	33.99812204699727	35.0	32.0	39.0	27.0	39.0
13	34.25869301310293	37.0	32.0	39.0	27.0	39.0
14	34.79662538399722	37.0	33.0	39.0	27.0	40.0
15	34.83509064899092	37.0	33.0	39.0	27.0	40.0
16	34.88733706834153	37.0	33.0	39.0	27.0	40.0
17	34.7758909590384	37.0	33.0	39.0	27.0	40.0
18	34.924964521221725	37.0	33.0	39.0	27.0	40.0
19	34.918336116541376	37.0	33.0	39.0	27.0	40.0
20	34.936924716597225	37.0	33.0	39.0	27.0	40.0
21	34.84735291267946	37.0	33.0	39.0	27.0	40.0
22	34.58451643422605	37.0	32.0	39.0	26.0	40.0
23	34.62613631830021	37.0	32.0	39.0	26.0	40.0
24	34.53123842308942	37.0	32.0	39.0	26.0	40.0
25	34.44005368836809	37.0	32.0	39.0	25.0	40.0
26	34.178738950284114	37.0	32.0	39.0	25.0	40.0
27	33.988692386168694	36.0	32.0	39.0	24.0	40.0
28	33.91730452475535	36.0	32.0	39.0	24.0	40.0
29	33.78123129883675	36.0	31.0	39.0	24.0	40.0
30	33.665037587557066	36.0	31.0	39.0	24.0	40.0
31	33.640419020044796	36.0	31.0	39.0	24.0	40.0
32	33.43779387542247	36.0	31.0	39.0	24.0	40.0
33	33.24597764694484	36.0	31.0	39.0	23.0	40.0
34	33.11715406053905	36.0	30.0	39.0	23.0	40.0
35	32.996477769481984	36.0	30.0	39.0	22.0	40.0
36	33.02458437109947	36.0	30.0	39.0	22.0	40.0
37	32.911340100423466	35.0	30.0	39.0	21.0	40.0
38	32.78008001960594	35.0	30.0	38.0	21.0	40.0
39	32.67063725015246	35.0	30.0	38.0	20.0	40.0
40	32.375542155627876	35.0	30.0	38.0	19.0	40.0
41	32.347637882786096	35.0	30.0	38.0	19.0	40.0
42	32.19035433183059	35.0	30.0	38.0	18.0	40.0
43	31.365673640835077	34.0	28.0	38.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	12.0
11	6.0
12	11.0
13	7.0
14	18.0
15	19.0
16	59.0
17	129.0
18	216.0
19	374.0
20	649.0
21	1152.0
22	1831.0
23	2951.0
24	4269.0
25	6065.0
26	8458.0
27	11107.0
28	14515.0
29	17892.0
30	21417.0
31	25397.0
32	28816.0
33	31648.0
34	34091.0
35	34202.0
36	33732.0
37	31255.0
38	28003.0
39	12602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.732971611277975	26.34748114922745	17.645918943102867	20.273628296391706
2	16.299435189248648	25.475472622921856	37.058082607134516	21.167009580694984
3	17.649338584382498	30.694700125956786	33.44323680445921	18.212724485201505
4	13.077848133730772	19.593119681745385	36.10115298905145	31.227879195472397
5	11.842787691571154	40.92598186450241	32.84707934137709	14.384151102549342
6	25.642180135303803	40.70285027100657	17.42449717024684	16.230472423442784
7	22.37699265347065	35.698775198481684	22.027904272841777	19.89632787520589
8	26.38338738266356	32.54928557994267	23.327937899314364	17.739389138079414
9	23.78616983078475	14.281276894053812	19.40503941136575	42.52751386379568
10	15.084037684446901	27.51728343696746	33.19559778179269	24.203081096792946
11	31.470958696432742	23.353300238804948	23.32195352707501	21.853787537687296
12	19.60366357569091	29.78707033632172	27.67202220487071	22.93724388311666
13	31.760773294881368	20.514997976712245	24.690664949246823	23.033563779159564
14	22.08432835395567	24.412249155063634	27.188427933909733	26.314994557070964
15	24.85024820896288	29.822691599651197	22.762842177855543	22.56421801353038
16	23.241306986897076	27.466273787879654	23.83062516741994	25.46179405780334
17	19.68544999629539	31.3202095100224	25.19164239671258	23.80269809696963
18	22.41802834882621	24.144662224932606	27.551194879657125	25.886114546584064
19	24.47437263831024	27.709923229053274	29.17723430812108	18.638469824515408
20	24.362664356509004	25.57920174173729	26.65353904375431	23.404594857999395
21	25.120969810266903	25.568372877685132	27.68684065041577	21.623816661632194
22	21.271878579936963	32.183953903235555	27.374513413542918	19.169654103284564
23	25.411639319035434	24.336447106698508	27.887459605487386	22.364453968778676
24	20.513573126179065	28.516388630832623	29.488136694460753	21.48190154852756
25	23.10423636560525	26.32126389941695	29.60326461754162	20.971235117436184
26	20.48935066711502	28.605299304103	29.769117219603665	21.136232809178317
27	22.905042261066814	29.56935317485196	28.663433205856702	18.862171358224522
28	21.42604740762694	25.443840941085284	30.16836033900044	22.96175131228734
29	20.12031437902164	28.370198966128452	28.831565568771833	22.677921086078072
30	23.540240628758045	25.127809092826165	31.938594641422114	19.39335563699368
31	21.697338949144235	26.16538525108716	26.65809856546048	25.47917723430812
32	21.87060077397881	26.846748776053392	32.045458431410545	19.237192018557252
33	20.281892429484145	28.191522709267797	31.63339165721516	19.893193204032897
34	20.662327521842958	29.527747539283126	28.38473244156688	21.425192497307034
35	20.55118918025499	24.9944430829206	35.783126350045876	18.67124138677853
36	23.01418581190833	26.557789087924675	26.474862786893656	23.95316231327334
37	22.17067429626632	24.74594914993417	30.76850738357546	22.314869170224043
38	21.710732544156116	24.591780322244198	29.22339946539608	24.474087668203605
39	25.37174350410642	25.66298295308822	28.236547986116257	20.728725556689103
40	20.442900539733383	23.989353516816088	34.566873934924224	21.000872008526304
41	23.297161127797693	26.10953111018654	27.288737411445542	23.304570350570224
42	20.111480305715933	24.873900727813652	33.46204483149718	21.55257413497324
43	23.354155149124857	23.722336526898328	30.170070159640254	22.75343816433656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	15.5
2	11.0
3	60.5
4	110.0
5	110.0
6	147.0
7	184.0
8	207.5
9	231.0
10	337.0
11	443.0
12	443.0
13	763.5
14	1084.0
15	2836.5
16	4589.0
17	4818.5
18	5048.0
19	5048.0
20	4780.0
21	4512.0
22	4753.5
23	4995.0
24	6145.0
25	7295.0
26	7295.0
27	8174.0
28	9053.0
29	10342.5
30	11632.0
31	13293.0
32	14954.0
33	14954.0
34	16191.5
35	17429.0
36	18471.0
37	19513.0
38	20088.0
39	20663.0
40	20663.0
41	21470.5
42	22278.0
43	22828.0
44	23378.0
45	23928.5
46	24479.0
47	24479.0
48	25455.5
49	26432.0
50	30900.5
51	35369.0
52	28368.5
53	21368.0
54	21368.0
55	26869.0
56	32370.0
57	26721.5
58	21073.0
59	15169.0
60	9265.0
61	9265.0
62	7597.0
63	5929.0
64	4645.5
65	3362.0
66	2628.0
67	1894.0
68	1894.0
69	1434.0
70	974.0
71	721.0
72	468.0
73	351.5
74	235.0
75	235.0
76	181.0
77	127.0
78	98.0
79	69.0
80	53.5
81	38.0
82	38.0
83	29.0
84	20.0
85	14.5
86	9.0
87	8.5
88	8.0
89	8.0
90	5.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	350914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.57446201010442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.91086999572404	42.094106988479375
2	7.870895179193998	7.803907880929338
3	2.319497804356402	3.449635673537612
4	1.062634480003107	2.1071813063816434
5	0.6422533996830722	1.5919683381724437
6	0.4819407264860966	1.4335171341784272
7	0.35391752324834824	1.2281689567689844
8	0.2818639672604396	1.1178603629575985
9	0.19957592828886445	0.8904482347578871
>10	1.657630871069159	16.06942569552028
>50	0.15093966491542937	5.229930043987847
>100	0.059914981493147536	4.67386441513003
>500	0.0023044223651210592	0.7945426705097307
>1k	0.004032739138961854	4.759544789376227
>5k	0.0017283167738407945	6.75589750931261
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	9676	2.7573707518081356	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	8080	2.302558461617376	RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	5899	1.6810386590446664	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	3204	0.9130442216611477	RNA PCR Primer, Index 29 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2995	0.8534854693742625	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2705	0.7708441384498766	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	2663	0.7588753939711724	RNA PCR Primer, Index 29 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	2598	0.7403523370398445	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	1448	0.4126367144086585	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.2997885521808762	No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT	768	0.2188570418963051	No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT	702	0.20004901485834137	No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA	696	0.19833919421852647	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	616	0.17554158568766137	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	399	0.11370307254768976	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	376	0.10714876009506602	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	359	0.1023042682822572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	2.849701066358139E-4	0.0
23	0.0	2.849701066358139E-4	0.0	2.849701066358139E-4	0.0
24	0.0	2.849701066358139E-4	0.0	2.849701066358139E-4	0.0
25	0.0	2.849701066358139E-4	0.0	5.699402132716278E-4	0.0
26	0.0	2.849701066358139E-4	0.0	8.549103199074417E-4	0.0
27	0.0	2.849701066358139E-4	0.0	0.0011398804265432556	0.0
28	0.0	2.849701066358139E-4	0.0	0.0037046113862655807	0.0
29	0.0	2.849701066358139E-4	0.0	0.010828864052160929	0.0
30	0.0	2.849701066358139E-4	0.0	0.019377967251235344	0.0
31	0.0	2.849701066358139E-4	0.0	0.04189060567546465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCAACG	25	1.2318786E-4	37.0	2
CGTCTTC	290	0.0	32.53448	37
CTTATAC	380	0.0	31.157896	1
GCAACGC	30	3.5944284E-4	30.833334	3
GGTATCA	275	0.0	30.272726	1
CTTCGGT	25	0.005491206	29.6	2
GCACGTG	40	5.928555E-5	27.75	7
CCGTCTT	1155	0.0	27.38961	37
GTATCAA	1115	0.0	27.210762	1
GCCGTCT	1195	0.0	26.472803	36
ACGTGTG	45	1.3206541E-4	24.666668	9
AGTAGTG	45	1.3206541E-4	24.666668	5
TAGGACC	45	1.3206541E-4	24.666668	4
GACTATT	45	1.3206541E-4	24.666668	2
TTATACA	485	0.0	24.41237	2
ATACACA	1735	0.0	22.818443	1
TACACAT	1780	0.0	22.137642	2
CGTGTGT	80	6.931805E-7	20.8125	10
ATCACGC	45	0.0038205916	20.555557	28
GGATTCG	45	0.0038205916	20.555557	33
>>END_MODULE