FastQCFastQC Report
Fri 10 Feb 2017
ERR1631001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542483
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT209443.860766143823862No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT182543.364898070538616TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG178663.293375091938365No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC90871.6750755323208286No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC77601.4304595720050213RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC52090.9602144214657419No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG20050.3695968352925345No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA18950.34931970218421593No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT15410.2840642010901724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.24645933605292702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13160.24258824700497528No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11640.2145689358007532No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT5890.10857483091636051TruSeq Adapter, Index 8 (95% over 24bp)
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT5850.10783748062151256TruSeq Adapter, Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26200.033.5400731
TTATACA27950.031.5724532
TCGCCGA658.731149E-1131.3076921
CGCCGAG658.731149E-1131.3076922
CGTCTTC9600.031.02604337
TATACAC29200.030.6643853
GCGCACG250.00549381729.634
CTACGTT250.00549381729.630
CACGGTA358.8633323E-426.4285722
ACGGTAC358.8633323E-426.4285723
CCGTCTT30050.025.05657437
GCCGTCT30850.024.40680936
GCGCGCC603.721618E-521.58333411
TATCGCG603.721618E-521.5833347
ATCGCGC603.721618E-521.5833348
GCCGAGC957.1231625E-921.4210533
TGCCGTC36350.020.76478635
GTATCAA6350.020.685041
TTAGAAC450.003823242420.5555573
CACGACA450.003823242420.55555719
Produced by FastQC (version 0.11.5)