##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631001.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542483
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273033440679246	31.0	31.0	34.0	28.0	34.0
2	31.33421876814573	31.0	31.0	34.0	28.0	34.0
3	30.794332725633797	31.0	31.0	34.0	26.0	34.0
4	34.91662595878581	37.0	35.0	37.0	32.0	37.0
5	35.1073452992997	37.0	35.0	37.0	32.0	37.0
6	35.29363869466877	37.0	35.0	37.0	32.0	37.0
7	35.28512598551475	37.0	35.0	37.0	32.0	37.0
8	35.33406392458381	37.0	35.0	37.0	32.0	37.0
9	37.11092329160545	39.0	37.0	39.0	33.0	39.0
10	36.92283260489269	39.0	37.0	39.0	33.0	39.0
11	36.98017818069875	39.0	37.0	39.0	33.0	39.0
12	36.8803243603947	39.0	37.0	39.0	33.0	39.0
13	36.99992995172199	39.0	37.0	39.0	33.0	39.0
14	38.02582569407705	40.0	37.0	41.0	33.0	41.0
15	38.035798356814865	40.0	37.0	41.0	33.0	41.0
16	38.036246297118986	40.0	37.0	41.0	33.0	41.0
17	37.95510827067392	40.0	37.0	41.0	33.0	41.0
18	38.05133617090305	40.0	37.0	41.0	33.0	41.0
19	38.05550404344468	40.0	37.0	41.0	33.0	41.0
20	38.07955456668688	40.0	37.0	41.0	33.0	41.0
21	38.052215461129656	40.0	37.0	41.0	33.0	41.0
22	37.945644379639546	40.0	37.0	41.0	33.0	41.0
23	37.940905060619414	40.0	37.0	41.0	33.0	41.0
24	37.88837069548723	40.0	37.0	41.0	33.0	41.0
25	37.82094001102339	40.0	37.0	41.0	32.0	41.0
26	37.67349391593838	39.0	37.0	41.0	32.0	41.0
27	37.600451995730744	39.0	37.0	41.0	32.0	41.0
28	37.61595662905566	39.0	37.0	41.0	32.0	41.0
29	37.57915178908832	39.0	37.0	41.0	32.0	41.0
30	37.495080951845495	39.0	37.0	41.0	32.0	41.0
31	37.46320345522348	39.0	37.0	41.0	32.0	41.0
32	37.3380511463032	39.0	36.0	41.0	31.0	41.0
33	37.13573144227561	39.0	36.0	40.0	31.0	41.0
34	37.14917333815069	39.0	36.0	40.0	31.0	41.0
35	37.17080535242579	39.0	36.0	40.0	31.0	41.0
36	37.13687986535984	39.0	36.0	40.0	31.0	41.0
37	37.10021327857279	39.0	36.0	40.0	31.0	41.0
38	37.05765157617842	39.0	36.0	40.0	31.0	41.0
39	36.90731691131335	39.0	36.0	40.0	30.0	41.0
40	36.701127961613544	39.0	35.0	40.0	30.0	41.0
41	36.71788056031249	39.0	35.0	40.0	30.0	41.0
42	36.701113214607645	39.0	35.0	40.0	30.0	41.0
43	35.855862764363124	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	7.0
10	19.0
11	15.0
12	16.0
13	17.0
14	9.0
15	7.0
16	12.0
17	14.0
18	24.0
19	43.0
20	80.0
21	156.0
22	279.0
23	578.0
24	981.0
25	1601.0
26	2600.0
27	3896.0
28	5667.0
29	8006.0
30	10638.0
31	13861.0
32	17796.0
33	22604.0
34	29666.0
35	39199.0
36	53302.0
37	77925.0
38	130974.0
39	122484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.8576195014406	23.906186921986496	16.589275608636584	26.646917967936325
2	12.17734011941388	20.65815887318128	42.771294215671276	24.393206791733565
3	13.242626957895453	28.829290503112542	36.588611993371224	21.339470545620784
4	10.413598214137586	20.244136682624156	34.33213575356279	35.01012934967547
5	12.00111339894522	37.79381105030019	35.55429386727326	14.650781683481325
6	27.059834133051176	39.48621431454995	17.121273846369377	16.332677706029497
7	22.252125873068834	34.77952304496178	21.07844854124461	21.88990254072478
8	25.040047337888925	34.37158399433715	23.270406630253852	17.317962037520072
9	21.321221125823296	14.290217389300677	18.113378668087297	46.27518281678873
10	13.689645574147026	28.33084170379533	32.819830298829636	25.15968242322801
11	28.681820444142954	21.86501696827366	25.64485891723796	23.808303670345428
12	18.043883402797874	27.19016079766555	26.811531421261126	27.95442437827545
13	29.69475541169032	18.771832481386515	26.49189006844454	25.041522038478625
14	21.705011954291656	23.874296521734323	23.78489279848401	30.635798725490016
15	24.494039444554023	28.910583373119525	20.46239236989915	26.132984812427303
16	27.60842275241805	26.077683540313707	21.40380435884627	24.91008934842198
17	18.610352766814813	34.30725018111166	22.92698573042842	24.1554113216451
18	25.453885190872338	23.351699500260835	23.39612485552543	27.798290453341394
19	24.989538842691843	27.554411843320437	23.3203621127298	24.13568720125792
20	19.616835919282263	24.659390248173676	24.76814941666375	30.95562441588031
21	25.167424601323912	23.671340853077425	26.581109454121144	24.58012509147752
22	19.046495466217376	33.69045665947136	23.34045490826441	23.922592966046864
23	22.91352908754744	26.783327772483194	25.620342019934267	24.682801120035098
24	26.199715014111042	26.964162932294656	26.840841095481334	19.995280958112975
25	22.729007176261746	30.00130879677336	23.74526022013593	23.52442380682897
26	26.21280298184459	28.879245985588486	26.261283763730848	18.646667268836072
27	19.923942317086436	31.041525725230102	24.730728889200215	24.30380306848325
28	19.142904017268744	25.641725178484855	31.164294549322285	24.051076254924116
29	23.186164359067472	23.826368752569206	27.10389081316834	25.88357607519498
30	20.511241826932824	26.756598824294954	28.265401865127572	24.46675748364465
31	20.54884669197007	24.993778606887222	31.242269343002455	23.215105358140256
32	25.321162137799707	24.91230139930652	26.53871181216739	23.227824650726383
33	18.795059015674223	32.174648790837686	29.436314133346116	19.593978060141975
34	20.248007771672107	30.014028089359485	29.36810923107268	20.369854907895732
35	24.562797359548593	23.54875636655895	33.36546951701712	18.52297675687533
36	23.192616174147393	28.488450329319075	23.11150764171412	25.207425854819416
37	20.664795025834913	27.01762082867113	28.339874244907215	23.977709900586746
38	21.3966151934715	23.09196785890065	29.477237074710178	26.03417987291768
39	25.715828883117076	24.365003142955633	25.83509529330873	24.08407268061856
40	19.413695913051654	22.402360995644102	36.48556728966622	21.698375801638022
41	21.69505772531121	25.67177220299991	25.448355063660983	27.1848150080279
42	22.513332215018718	23.026712357806602	33.19790666251293	21.26204876466175
43	22.44125622369733	22.451763465398916	31.98902085410971	23.117959456794036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	11.0
2	11.0
3	51.5
4	92.0
5	92.0
6	137.0
7	182.0
8	186.5
9	191.0
10	262.0
11	333.0
12	333.0
13	549.0
14	765.0
15	1607.0
16	2449.0
17	2800.0
18	3151.0
19	3151.0
20	3496.5
21	3842.0
22	4885.0
23	5928.0
24	7609.0
25	9290.0
26	9290.0
27	11641.5
28	13993.0
29	15974.0
30	17955.0
31	19980.5
32	22006.0
33	22006.0
34	24378.5
35	26751.0
36	28188.5
37	29626.0
38	31224.5
39	32823.0
40	32823.0
41	33391.5
42	33960.0
43	33785.0
44	33610.0
45	33648.5
46	33687.0
47	33687.0
48	34785.0
49	35883.0
50	53010.5
51	70138.0
52	49665.0
53	29192.0
54	29192.0
55	41924.0
56	54656.0
57	45182.5
58	35709.0
59	25225.0
60	14741.0
61	14741.0
62	12897.0
63	11053.0
64	8891.0
65	6729.0
66	5558.0
67	4387.0
68	4387.0
69	3626.5
70	2866.0
71	2420.0
72	1974.0
73	2085.0
74	2196.0
75	2196.0
76	1824.5
77	1453.0
78	1028.5
79	604.0
80	382.0
81	160.0
82	160.0
83	105.5
84	51.0
85	35.5
86	20.0
87	16.0
88	12.0
89	12.0
90	7.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	542483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.368018118271536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30575104047004	36.90757948853772
2	13.842778174708158	13.390954911229958
3	4.3193945738612225	6.267616650254501
4	1.8078677806767685	3.497719262848532
5	0.985556385488732	2.38347045549486
6	0.5655310704755718	1.6412170237924775
7	0.3831848986600216	1.2973725884725174
8	0.2715480005816537	1.0507390889691068
9	0.19281167327357002	0.8393326655679257
>10	1.202554730286293	11.06540598854813
>50	0.08912405926659175	2.9889737381849986
>100	0.028184531534881718	2.0757939017613585
>500	0.0011426161433060157	0.3128060033005668
>1k	0.0022852322866120313	1.7055111770062703
>5k	0.0011426161433060157	4.063162078208069
>10k+	0.0011426161433060157	10.512344977823052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	20944	3.860766143823862	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	18254	3.364898070538616	TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	17866	3.293375091938365	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	9087	1.6750755323208286	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	7760	1.4304595720050213	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	5209	0.9602144214657419	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	2005	0.3695968352925345	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1895	0.34931970218421593	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1541	0.2840642010901724	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1337	0.24645933605292702	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1316	0.24258824700497528	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	1164	0.2145689358007532	No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT	589	0.10857483091636051	TruSeq Adapter, Index 8 (95% over 24bp)
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT	585	0.10783748062151256	TruSeq Adapter, Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.8433757371198728E-4	0.0	0.0	0.0	0.0
7	1.8433757371198728E-4	0.0	0.0	0.0	0.0
8	1.8433757371198728E-4	0.0	0.0	1.8433757371198728E-4	0.0
9	1.8433757371198728E-4	0.0	0.0	1.8433757371198728E-4	0.0
10	1.8433757371198728E-4	0.0	0.0	3.6867514742397456E-4	0.0
11	1.8433757371198728E-4	0.0	0.0	3.6867514742397456E-4	0.0
12	1.8433757371198728E-4	0.0	0.0	3.6867514742397456E-4	0.0
13	1.8433757371198728E-4	0.0	0.0	3.6867514742397456E-4	0.0
14	1.8433757371198728E-4	0.0	0.0	3.6867514742397456E-4	0.0
15	1.8433757371198728E-4	0.0	0.0	3.6867514742397456E-4	0.0
16	1.8433757371198728E-4	0.0	0.0	5.530127211359619E-4	0.0
17	1.8433757371198728E-4	0.0	0.0	9.216878685599365E-4	0.0
18	1.8433757371198728E-4	0.0	0.0	9.216878685599365E-4	0.0
19	1.8433757371198728E-4	0.0	0.0	9.216878685599365E-4	0.0
20	1.8433757371198728E-4	0.0	0.0	0.0011060254422719238	0.0
21	1.8433757371198728E-4	0.0	0.0	0.0022120508845438476	0.0
22	1.8433757371198728E-4	0.0	0.0	0.002580726031967822	0.0
23	1.8433757371198728E-4	0.0	0.0	0.0029494011793917965	0.0
24	1.8433757371198728E-4	0.0	0.0	0.0029494011793917965	0.0
25	1.8433757371198728E-4	0.0	0.0	0.0029494011793917965	0.0
26	1.8433757371198728E-4	0.0	0.0	0.0035024139005277585	0.0
27	1.8433757371198728E-4	0.0	0.0	0.00479277691651167	0.0
28	3.6867514742397456E-4	0.0	0.0	0.009954228980447314	0.0
29	3.6867514742397456E-4	0.0	0.0	0.01972412038718264	0.0
30	3.6867514742397456E-4	0.0	0.0	0.03410245113671765	0.0
31	3.6867514742397456E-4	0.0	0.0	0.0768687682378987	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2620	0.0	33.540073	1
TTATACA	2795	0.0	31.572453	2
TCGCCGA	65	8.731149E-11	31.307692	1
CGCCGAG	65	8.731149E-11	31.307692	2
CGTCTTC	960	0.0	31.026043	37
TATACAC	2920	0.0	30.664385	3
GCGCACG	25	0.005493817	29.6	34
CTACGTT	25	0.005493817	29.6	30
CACGGTA	35	8.8633323E-4	26.428572	2
ACGGTAC	35	8.8633323E-4	26.428572	3
CCGTCTT	3005	0.0	25.056574	37
GCCGTCT	3085	0.0	24.406809	36
GCGCGCC	60	3.721618E-5	21.583334	11
TATCGCG	60	3.721618E-5	21.583334	7
ATCGCGC	60	3.721618E-5	21.583334	8
GCCGAGC	95	7.1231625E-9	21.421053	3
TGCCGTC	3635	0.0	20.764786	35
GTATCAA	635	0.0	20.68504	1
TTAGAAC	45	0.0038232424	20.555557	3
CACGACA	45	0.0038232424	20.555557	19
>>END_MODULE