FastQCFastQC Report
Fri 10 Feb 2017
ERR1631000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061792
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT168871.5904244899189295No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG137211.2922493294355204No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT113821.071961363430879TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC78180.736302401977035No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA67570.636376992857358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41740.3931090081673247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40950.3856687562159067No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC40520.3816189988246285RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC39260.3697522678641391No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20590.19391745275910913No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG19250.18129727856303307No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC18570.1748930110605467No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16230.15285479641963773No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA14420.13580814321449022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14340.1350546999789036No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG14140.133171091889937No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA13240.12469485548958742No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC12550.11819640758265273No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG12460.11734878394261776No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT12330.1161244386847895No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT11770.11085033603568308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG900.032.888891
CTTATAC18850.031.2095491
CGTCTTC6000.030.21666737
GGTATCA5700.029.8596481
TATACAC21950.028.0660613
TTATACA22000.026.9931812
TTACGGG1100.026.909093
GTATCAA20400.025.3014681
TCGCCGA451.3231194E-424.6666661
CGGCGTA451.3231194E-424.66666612
CCGTCTT17500.024.31428537
GGACCGT1150.024.1304366
GCCGTCT18000.023.6388936
GCTTTAT11100.023.3333341
GACGATT400.001931090523.12500214
GCGGGTA753.740206E-722.222
AGGACCG502.7019146E-422.1999995
ATCGGCG502.7019146E-422.19999910
CGCCGAG502.7019146E-422.1999992
ATTGAGC12200.021.229516
Produced by FastQC (version 0.11.5)