##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631000.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1061792
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477313824176488	31.0	31.0	34.0	28.0	34.0
2	31.469585380187457	31.0	31.0	34.0	28.0	34.0
3	30.93725418914439	31.0	31.0	34.0	27.0	34.0
4	35.087318420180225	37.0	35.0	37.0	32.0	37.0
5	35.29838989180555	37.0	35.0	37.0	33.0	37.0
6	35.48861735631837	37.0	35.0	37.0	33.0	37.0
7	35.503302906784	37.0	35.0	37.0	33.0	37.0
8	35.52941913293753	37.0	35.0	37.0	33.0	37.0
9	37.29882029625388	39.0	37.0	39.0	34.0	39.0
10	37.1447006570025	39.0	37.0	39.0	33.0	39.0
11	37.18694998643802	39.0	37.0	39.0	33.0	39.0
12	37.12732625598987	39.0	37.0	39.0	33.0	39.0
13	37.20652255809048	39.0	37.0	39.0	34.0	39.0
14	38.32276283867274	40.0	38.0	41.0	34.0	41.0
15	38.334111577408756	40.0	38.0	41.0	34.0	41.0
16	38.325632515596276	40.0	38.0	41.0	34.0	41.0
17	38.32206307826768	40.0	38.0	41.0	34.0	41.0
18	38.36003002471294	40.0	38.0	41.0	34.0	41.0
19	38.38774543413399	40.0	38.0	41.0	34.0	41.0
20	38.38196275730086	40.0	38.0	41.0	34.0	41.0
21	38.32837787438594	40.0	38.0	41.0	34.0	41.0
22	38.27693277026009	40.0	38.0	41.0	34.0	41.0
23	38.27713054910943	40.0	38.0	41.0	34.0	41.0
24	38.230536677616705	40.0	38.0	41.0	34.0	41.0
25	38.20131155631235	40.0	38.0	41.0	33.0	41.0
26	38.06961627136012	40.0	37.0	41.0	33.0	41.0
27	38.01942470841747	40.0	37.0	41.0	33.0	41.0
28	37.98267834001386	40.0	37.0	41.0	33.0	41.0
29	37.92172949127513	40.0	37.0	41.0	33.0	41.0
30	37.85815866007655	40.0	37.0	41.0	33.0	41.0
31	37.76981084807571	40.0	37.0	41.0	33.0	41.0
32	37.727006796057985	40.0	37.0	41.0	33.0	41.0
33	37.64889733582472	40.0	37.0	41.0	33.0	41.0
34	37.6201854977246	40.0	37.0	41.0	32.0	41.0
35	37.59445823664145	40.0	37.0	41.0	32.0	41.0
36	37.526682250384255	40.0	37.0	41.0	32.0	41.0
37	37.50041156836744	40.0	37.0	41.0	32.0	41.0
38	37.45285140592508	40.0	37.0	41.0	32.0	41.0
39	37.372508928302345	39.0	36.0	41.0	31.0	41.0
40	37.241220502697324	39.0	36.0	41.0	31.0	41.0
41	37.225126955185196	39.0	36.0	41.0	31.0	41.0
42	37.154854246406074	39.0	36.0	41.0	31.0	41.0
43	36.47518440523191	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	11.0
11	15.0
12	13.0
13	10.0
14	8.0
15	11.0
16	7.0
17	15.0
18	20.0
19	40.0
20	93.0
21	170.0
22	389.0
23	776.0
24	1388.0
25	2413.0
26	3768.0
27	5981.0
28	9006.0
29	12671.0
30	17350.0
31	23144.0
32	30305.0
33	39017.0
34	51443.0
35	69951.0
36	97727.0
37	145251.0
38	253416.0
39	297372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.000576384075224	21.887620174196076	15.485047918989784	23.626755522738918
2	13.77039947560351	22.193894849462044	40.1571117507007	23.878593924233748
3	14.813918356890992	28.182826768331275	34.4484607154697	22.554794159308038
4	11.564223501401404	18.592624544166842	38.88077890961695	30.962373044814807
5	12.854306681534613	37.67357448539827	35.84995931406528	13.622159519001839
6	27.838597390072632	40.50463744311504	17.198189475904886	14.458575690907447
7	24.739967903318163	32.733529730870075	22.98585787046804	19.54064449534372
8	25.593430728428917	33.51051806756879	22.66912917030831	18.22692203369398
9	24.527496910882736	15.033547060064494	19.66684623730448	40.77210979174829
10	14.989470630782678	29.079330038274914	32.92716464241584	23.004034688526566
11	31.996473885657455	23.51110198607637	24.886324251830867	19.60609987643531
12	21.537363250052742	26.512443115035712	28.440127633284106	23.51006600162744
13	29.510111208221574	20.71130692263645	26.110952050872488	23.667629818269493
14	21.672512130436093	22.107154696965132	27.378526114342545	28.84180705825623
15	24.27368072089449	29.252998704077637	22.64370046110726	23.829620113920615
16	24.12421641903499	27.110206142069256	24.212934360025315	24.552643078870435
17	21.942809891202796	29.685851842922155	24.861648985865408	23.509689280009642
18	23.880383351918265	25.268790874295533	26.75712380579247	24.09370196799373
19	25.321626081191045	26.256743316958502	26.104924504987792	22.316706096862664
20	25.168677104366953	25.724906573038787	25.337165847925018	23.76925047466924
21	24.50301000572617	26.23527018474428	26.142879207980467	23.118840601549078
22	21.815477984388657	29.39125553780778	27.635450257677586	21.157816220125973
23	23.464011783852204	25.61659910792321	26.041164371176272	24.87822473704831
24	23.041141767879207	27.811473433591516	26.727174437177904	22.420210361351376
25	23.82095551671137	28.095333172598774	26.04144691238962	22.042264398300233
26	23.741278894548085	27.058312739218227	26.856955034507703	22.34345333172599
27	23.548585787046804	27.6545688797806	27.222563364576114	21.574281968596488
28	21.767728519333353	26.966204303667762	27.38549546427172	23.880571712727164
29	22.12570823664145	27.210131551188933	27.330682468882795	23.33347774328682
30	25.02081386938308	25.36476070642838	26.728963864862422	22.88546155932612
31	22.046785057713752	28.57970299267653	26.798751544558634	22.574760405051084
32	23.260958831861608	26.661248154064072	28.673789216720415	21.40400379735391
33	20.63389063017992	26.43380247732136	29.56379403875712	23.3685128537416
34	22.64266447665833	28.682547994334108	26.572718573882643	22.10206895512492
35	23.40561993309424	25.280375064042676	29.395587836412407	21.91841716645068
36	22.597834604140925	26.03118124830475	28.11652376359965	23.254460383954672
37	22.627689792351045	26.388878424399504	28.373730448148038	22.609701335101416
38	22.70520026521202	25.048126186673098	28.444083210270936	23.802590337843945
39	24.54454356408788	25.428803381453243	26.81796434706609	23.208688707392785
40	21.79654772309454	24.651344142732288	31.080569452397455	22.471538681775716
41	22.402598625719538	25.56753111720563	26.549267653175008	25.480602603899822
42	22.622133148488594	24.963175461860704	29.315440312226876	23.099251077423826
43	23.079284831680784	25.21407205931105	29.115401133178626	22.59124197582954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	42.5
2	54.0
3	172.0
4	290.0
5	290.0
6	349.0
7	408.0
8	427.5
9	447.0
10	653.5
11	860.0
12	860.0
13	1304.0
14	1748.0
15	4246.0
16	6744.0
17	7513.5
18	8283.0
19	8283.0
20	8489.5
21	8696.0
22	10611.0
23	12526.0
24	16244.0
25	19962.0
26	19962.0
27	24151.0
28	28340.0
29	32492.5
30	36645.0
31	41167.5
32	45690.0
33	45690.0
34	49813.0
35	53936.0
36	56841.5
37	59747.0
38	61580.5
39	63414.0
40	63414.0
41	64356.0
42	65298.0
43	65698.0
44	66098.0
45	68663.0
46	71228.0
47	71228.0
48	74602.0
49	77976.0
50	89194.5
51	100413.0
52	83018.5
53	65624.0
54	65624.0
55	72852.5
56	80081.0
57	67882.0
58	55683.0
59	47564.5
60	39446.0
61	39446.0
62	35713.0
63	31980.0
64	28036.0
65	24092.0
66	19599.5
67	15107.0
68	15107.0
69	12805.5
70	10504.0
71	8970.0
72	7436.0
73	4626.5
74	1817.0
75	1817.0
76	1237.0
77	657.0
78	470.0
79	283.0
80	209.0
81	135.0
82	135.0
83	98.0
84	61.0
85	46.0
86	31.0
87	22.0
88	13.0
89	13.0
90	10.5
91	8.0
92	4.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1061792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.695911026777935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29818774509486	34.86515198704254
2	13.85817430767127	12.665238003138526
3	4.341594159821347	5.95179301324725
4	1.7861646247727847	3.264816790911815
5	0.8701976054634056	1.9882236177485497
6	0.5521072267089904	1.5137425625361083
7	0.3404040377362839	1.0888550835087234
8	0.243350028278611	0.8896080992466643
9	0.19068337508976227	0.7842105488148751
>10	1.2400278788106998	11.142829900680159
>50	0.15660202403639484	5.040351906461458
>100	0.10949191044411467	9.548813036988735
>500	0.0084701288719614	2.587289353052167
>1k	0.0035120046542278967	3.329198610746806
>5k	4.131770181444584E-4	1.3759164564065869
>10k+	6.197655272166877E-4	3.963961029469131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	16887	1.5904244899189295	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	13721	1.2922493294355204	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	11382	1.071961363430879	TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	7818	0.736302401977035	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	6757	0.636376992857358	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4174	0.3931090081673247	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4095	0.3856687562159067	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	4052	0.3816189988246285	RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	3926	0.3697522678641391	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2059	0.19391745275910913	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	1925	0.18129727856303307	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1857	0.1748930110605467	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1623	0.15285479641963773	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1442	0.13580814321449022	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1434	0.1350546999789036	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1414	0.133171091889937	No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA	1324	0.12469485548958742	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	1255	0.11819640758265273	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	1246	0.11734878394261776	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1233	0.1161244386847895	No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT	1177	0.11085033603568308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.418040444832887E-5	0.0
8	0.0	0.0	0.0	9.418040444832887E-5	0.0
9	0.0	0.0	0.0	9.418040444832887E-5	0.0
10	0.0	0.0	0.0	9.418040444832887E-5	0.0
11	0.0	0.0	0.0	9.418040444832887E-5	0.0
12	0.0	0.0	0.0	1.8836080889665774E-4	0.0
13	0.0	0.0	0.0	1.8836080889665774E-4	0.0
14	0.0	0.0	0.0	1.8836080889665774E-4	0.0
15	0.0	0.0	0.0	2.8254121334498657E-4	0.0
16	0.0	0.0	0.0	2.8254121334498657E-4	0.0
17	0.0	0.0	0.0	2.8254121334498657E-4	0.0
18	0.0	0.0	0.0	3.767216177933155E-4	0.0
19	0.0	0.0	0.0	4.7090202224164433E-4	0.0
20	0.0	0.0	0.0	5.650824266899731E-4	0.0
21	0.0	0.0	0.0	8.476236400349597E-4	0.0
22	0.0	0.0	0.0	0.0012243452578282752	0.0
23	0.0	0.0	0.0	0.0017894276845182484	0.0
24	0.0	0.0	0.0	0.002166149302311564	0.0
25	0.0	0.0	0.0	0.0025428709201048795	0.0
26	0.0	0.0	0.0	0.002731231729001537	0.0
27	0.0	0.0	0.0	0.0031079533467948525	0.0
28	0.0	0.0	0.0	0.012149272173834423	0.0
29	0.0	0.0	0.0	0.030420270636810222	0.0
30	0.0	0.0	0.0	0.05113995961544257	0.0
31	0.0	0.0	0.0	0.10567041379102499	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	90	0.0	32.88889	1
CTTATAC	1885	0.0	31.209549	1
CGTCTTC	600	0.0	30.216667	37
GGTATCA	570	0.0	29.859648	1
TATACAC	2195	0.0	28.066061	3
TTATACA	2200	0.0	26.993181	2
TTACGGG	110	0.0	26.90909	3
GTATCAA	2040	0.0	25.301468	1
TCGCCGA	45	1.3231194E-4	24.666666	1
CGGCGTA	45	1.3231194E-4	24.666666	12
CCGTCTT	1750	0.0	24.314285	37
GGACCGT	115	0.0	24.130436	6
GCCGTCT	1800	0.0	23.63889	36
GCTTTAT	1110	0.0	23.333334	1
GACGATT	40	0.0019310905	23.125002	14
GCGGGTA	75	3.740206E-7	22.2	22
AGGACCG	50	2.7019146E-4	22.199999	5
ATCGGCG	50	2.7019146E-4	22.199999	10
CGCCGAG	50	2.7019146E-4	22.199999	2
ATTGAGC	1220	0.0	21.22951	6
>>END_MODULE