FastQCFastQC Report
Fri 10 Feb 2017
ERR1630999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858982
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT176442.054059340009453No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG139921.6289049130249527No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT128301.4936285044389754Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC71710.8348254096127742No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC54560.6351704692298559Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC36450.4243395088604883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23850.2776542465383442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23030.26810806279991894No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG15940.18556849852499818No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA14930.1738103941642549No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT11410.1328316542139416No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9890.11513628923539726No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9370.10908261174273734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8850.10302893425007742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20350.032.7272721
TTATACA22400.029.979912
CGTCTTC6850.029.97810237
TATACAC23550.029.0658173
GGTATCA4850.028.989691
GATTACG604.3201E-827.7500021
CGGCGTA358.8675646E-426.4285712
CGTATAC358.8675646E-426.428571
CCGTCTT20850.025.28777137
GCCGTCT21000.025.10714136
CGTAAAT400.00193079223.12515
ACGTGGG1300.022.7692336
GTATCAA13750.022.6036361
CGTGGGA1155.456968E-1222.5217437
TGCCGTC24300.021.92592435
CCGTTAT603.724874E-521.5833349
GTCGCCA909.471296E-820.55555712
CGAACGC450.003825032420.55555730
CGCACTC450.003825032420.55555734
ATGCCGT25850.020.53965234
Produced by FastQC (version 0.11.5)