##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630999.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	858982
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47358384692578	31.0	31.0	34.0	28.0	34.0
2	31.49171111851005	31.0	31.0	34.0	28.0	34.0
3	30.961882786833716	31.0	31.0	34.0	27.0	34.0
4	35.08413913213548	37.0	35.0	37.0	32.0	37.0
5	35.29320521268199	37.0	35.0	37.0	33.0	37.0
6	35.48395193380071	37.0	35.0	37.0	33.0	37.0
7	35.48306716555178	37.0	35.0	37.0	33.0	37.0
8	35.51788978115956	37.0	35.0	37.0	33.0	37.0
9	37.28917253213688	39.0	37.0	39.0	34.0	39.0
10	37.121684738446206	39.0	37.0	39.0	33.0	39.0
11	37.180994479511796	39.0	37.0	39.0	33.0	39.0
12	37.11313508315657	39.0	37.0	39.0	33.0	39.0
13	37.19516474151961	39.0	37.0	39.0	33.0	39.0
14	38.31660733286611	40.0	38.0	41.0	34.0	41.0
15	38.329405039919344	40.0	38.0	41.0	34.0	41.0
16	38.33513158599365	40.0	38.0	41.0	34.0	41.0
17	38.290831472603614	40.0	38.0	41.0	33.0	41.0
18	38.33510015343744	40.0	38.0	41.0	34.0	41.0
19	38.36871319771544	40.0	38.0	41.0	34.0	41.0
20	38.378982330246735	40.0	38.0	41.0	34.0	41.0
21	38.336237546304815	40.0	38.0	41.0	34.0	41.0
22	38.272169847563745	40.0	38.0	41.0	34.0	41.0
23	38.257560693937705	40.0	38.0	41.0	34.0	41.0
24	38.213933470084356	40.0	38.0	41.0	33.0	41.0
25	38.19125080618686	40.0	38.0	41.0	33.0	41.0
26	38.0651131222773	40.0	37.0	41.0	33.0	41.0
27	38.0125380974223	40.0	37.0	41.0	33.0	41.0
28	37.99721065167838	40.0	37.0	41.0	33.0	41.0
29	37.93970537217311	40.0	37.0	41.0	33.0	41.0
30	37.88972062278371	40.0	37.0	41.0	33.0	41.0
31	37.86154657489912	40.0	37.0	41.0	33.0	41.0
32	37.76736997981332	40.0	37.0	41.0	33.0	41.0
33	37.67619810426761	40.0	37.0	41.0	33.0	41.0
34	37.64160599407205	40.0	37.0	41.0	32.0	41.0
35	37.638747959794266	40.0	37.0	41.0	33.0	41.0
36	37.604091820317535	40.0	37.0	41.0	32.0	41.0
37	37.544204651552654	40.0	37.0	41.0	32.0	41.0
38	37.51581988912457	40.0	37.0	41.0	32.0	41.0
39	37.426339550770564	40.0	37.0	41.0	32.0	41.0
40	37.31244193708366	39.0	37.0	41.0	31.0	41.0
41	37.29235536949552	39.0	36.0	41.0	31.0	41.0
42	37.24421233506639	39.0	36.0	41.0	31.0	41.0
43	36.56118987359456	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	20.0
11	17.0
12	19.0
13	13.0
14	15.0
15	13.0
16	11.0
17	20.0
18	19.0
19	36.0
20	93.0
21	174.0
22	334.0
23	635.0
24	1113.0
25	1866.0
26	3118.0
27	4732.0
28	7127.0
29	10297.0
30	13886.0
31	18458.0
32	24189.0
33	31654.0
34	41750.0
35	55566.0
36	78430.0
37	116351.0
38	204263.0
39	244747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.0730702156739	22.799895690480128	15.505214311824927	24.621819782021042
2	13.301792121371578	22.020368296425303	41.84662775238596	22.831211829817157
3	14.157106900959507	28.915041293065514	34.93810114763755	21.989750658337428
4	11.24703428011297	19.71647834296877	37.75469101797243	31.281796358945822
5	11.924464074916587	38.66018147062453	36.194704894863925	13.220649559594962
6	26.877163898661436	40.620525226372614	17.8392562358699	14.663054639096046
7	23.67919234628898	33.84005718396893	23.051938224549527	19.428812245192564
8	24.344049118607842	35.59853407871178	22.588599062611323	17.46881774006906
9	23.392457583511643	15.212542288429793	19.715896258594476	41.679103869464086
10	14.021015574249517	29.20573422958805	33.933074266981144	22.840175929181285
11	30.18980607276986	23.450898854690784	25.728012926929782	20.63128214560957
12	19.709958997976678	27.05143996032513	29.375819283756822	23.862781757941377
13	28.512006072304192	20.950264382722807	27.23468012135295	23.303049423620052
14	21.43223024463842	22.823993983575907	26.646076402066633	29.097699369719038
15	23.669296911925976	29.298169228225966	22.97079566277291	24.06173819707514
16	24.577581369574684	27.924217271141888	23.759869240566157	23.738332118717274
17	20.24326470170504	31.50997343366916	25.078988849591727	23.167773015034076
18	23.145537391936035	26.84968951619475	25.93081112293389	24.073961968935322
19	22.77358547676203	28.864050701877336	26.26562605502793	22.096737766332705
20	21.872868115979145	25.987855391614723	26.679138794526548	25.46013769787958
21	23.14809856318293	26.370517659275748	26.96575714042902	23.5156266371123
22	20.860390555331776	30.49505111864975	25.702401214460842	22.942157111557634
23	23.662893983808743	25.808340570582388	26.579253115897654	23.94951232971122
24	22.44680330903325	28.664977845868716	26.270049896272564	22.61816894882547
25	21.203820336165368	28.517477665422557	27.593127679043334	22.68557431936874
26	22.512113175829064	29.924492014966553	26.579253115897654	20.98414169330673
27	20.546297826962615	29.428672544942735	26.506841819735456	23.518187808359198
28	20.790656847291327	26.508820906608054	29.484203394250404	23.21631885185021
29	20.503922084513995	28.362643221860296	26.590196302134387	24.54323839149132
30	21.937595898400662	26.183784992002163	28.623766272168684	23.25485283742849
31	21.649347716250166	26.343625361183353	28.059493679727865	23.947533242838617
32	21.728976858653613	26.327094164953397	29.022261234810507	22.92166774158248
33	22.414206584072772	27.71652956639371	28.809916855067975	21.059346994465542
34	20.839551934732043	28.38860418495382	26.916396385488873	23.85544749482527
35	21.011499658898554	26.150024098293095	32.614420325455015	20.224055917353333
36	22.382075526611732	28.480689932967163	25.55501745088954	23.58221708953156
37	21.666577413729275	27.31640476750386	28.14506008274911	22.87195773601775
38	21.90278725281787	25.479462899106153	28.806657182571925	23.81109266550405
39	23.78291978178821	26.091000742739663	27.003592624758145	23.122486850713987
40	20.466901518308884	25.354896843007186	32.24607733340163	21.9321243052823
41	21.788582298581343	26.513244747852692	26.842238836203787	24.85593411736218
42	22.552160580780505	25.34651482801735	30.22868930897271	21.87263528222943
43	22.296625540465342	24.8640832986023	30.18875832089613	22.650532840036227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	17.0
2	23.0
3	106.5
4	190.0
5	190.0
6	245.0
7	300.0
8	296.0
9	292.0
10	462.0
11	632.0
12	632.0
13	936.0
14	1240.0
15	2756.5
16	4273.0
17	4898.0
18	5523.0
19	5523.0
20	6101.0
21	6679.0
22	8355.5
23	10032.0
24	12902.5
25	15773.0
26	15773.0
27	19644.5
28	23516.0
29	28416.0
30	33316.0
31	36992.0
32	40668.0
33	40668.0
34	44180.5
35	47693.0
36	50159.5
37	52626.0
38	56340.5
39	60055.0
40	60055.0
41	61041.5
42	62028.0
43	60753.5
44	59479.0
45	58991.5
46	58504.0
47	58504.0
48	58671.0
49	58838.0
50	72673.5
51	86509.0
52	69710.5
53	52912.0
54	52912.0
55	58644.0
56	64376.0
57	54012.0
58	43648.0
59	33311.5
60	22975.0
61	22975.0
62	20147.5
63	17320.0
64	14331.5
65	11343.0
66	9364.0
67	7385.0
68	7385.0
69	5856.5
70	4328.0
71	3682.5
72	3037.0
73	2411.5
74	1786.0
75	1786.0
76	1317.5
77	849.0
78	669.0
79	489.0
80	341.5
81	194.0
82	194.0
83	137.0
84	80.0
85	59.0
86	38.0
87	24.5
88	11.0
89	11.0
90	10.5
91	10.0
92	5.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	858982.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.66447264172527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8437660374766	43.1025877737645
2	11.031394623246197	11.61925160672049
3	2.89077184391996	4.567229240627773
4	1.178517195730276	2.482639464493596
5	0.6465810856367902	1.7025925947587877
6	0.3946125232498956	1.246923626086578
7	0.2718316270664055	1.0021108500756062
8	0.19925567255041854	0.8394955932592069
9	0.1585966986084263	0.7517170345438273
>10	1.1601046669525459	12.320025651906262
>50	0.15140716564138662	5.5549745028950275
>100	0.06918232373331937	6.111801070115679
>500	0.0015472085174674753	0.5906347704619319
>1k	0.001326178729257836	1.4621528087844555
>5k	4.420595764192787E-4	1.4698354841590096
>10k+	6.63089364628918E-4	5.176027927347313
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	17644	2.054059340009453	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	13992	1.6289049130249527	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	12830	1.4936285044389754	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	7171	0.8348254096127742	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	5456	0.6351704692298559	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	3645	0.4243395088604883	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2385	0.2776542465383442	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2303	0.26810806279991894	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1594	0.18556849852499818	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1493	0.1738103941642549	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	1141	0.1328316542139416	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	989	0.11513628923539726	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	937	0.10908261174273734	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	885	0.10302893425007742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3283374971768908E-4	0.0
10	0.0	0.0	0.0	4.6566749943537816E-4	0.0
11	0.0	0.0	0.0	4.6566749943537816E-4	0.0
12	0.0	0.0	0.0	4.6566749943537816E-4	0.0
13	0.0	0.0	0.0	4.6566749943537816E-4	0.0
14	0.0	0.0	0.0	4.6566749943537816E-4	0.0
15	0.0	0.0	0.0	5.820843742942226E-4	0.0
16	0.0	0.0	0.0	5.820843742942226E-4	0.0
17	0.0	0.0	0.0	5.820843742942226E-4	0.0
18	0.0	0.0	0.0	5.820843742942226E-4	0.0
19	0.0	0.0	0.0	5.820843742942226E-4	0.0
20	0.0	0.0	0.0	5.820843742942226E-4	0.0
21	0.0	0.0	0.0	6.985012491530672E-4	0.0
22	0.0	0.0	0.0	0.0011641687485884453	0.0
23	0.0	0.0	0.0	0.0017462531228826681	0.0
24	0.0	0.0	0.0	0.0018626699977415126	0.0
25	0.0	0.0	0.0	0.001979086872600357	0.0
26	0.0	0.0	0.0	0.0020955037474592016	0.0
27	0.0	0.0	0.0	0.002794004996612269	0.0
28	0.0	0.0	0.0	0.008265598114977962	0.0
29	0.0	0.0	0.0	0.01688044685453246	0.0
30	0.0	0.0	0.0	0.031199722462170336	0.0
31	0.0	0.0	0.0	0.07276054678677783	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2035	0.0	32.727272	1
TTATACA	2240	0.0	29.97991	2
CGTCTTC	685	0.0	29.978102	37
TATACAC	2355	0.0	29.065817	3
GGTATCA	485	0.0	28.98969	1
GATTACG	60	4.3201E-8	27.750002	1
CGGCGTA	35	8.8675646E-4	26.42857	12
CGTATAC	35	8.8675646E-4	26.42857	1
CCGTCTT	2085	0.0	25.287771	37
GCCGTCT	2100	0.0	25.107141	36
CGTAAAT	40	0.001930792	23.125	15
ACGTGGG	130	0.0	22.76923	36
GTATCAA	1375	0.0	22.603636	1
CGTGGGA	115	5.456968E-12	22.52174	37
TGCCGTC	2430	0.0	21.925924	35
CCGTTAT	60	3.724874E-5	21.583334	9
GTCGCCA	90	9.471296E-8	20.555557	12
CGAACGC	45	0.0038250324	20.555557	30
CGCACTC	45	0.0038250324	20.555557	34
ATGCCGT	2585	0.0	20.539652	34
>>END_MODULE