FastQCFastQC Report
Fri 10 Feb 2017
ERR1630998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872937
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT170111.9487087842536173No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT141921.6257759723783045TruSeq Adapter, Index 3 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG129641.4851014448923576No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC64690.7410614969923374No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC60040.6877930480664699RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC38130.43680128119211353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.2958976420978834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25050.28696228937483464No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT23820.2728719254654116No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC20580.2357558449235168No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18970.21731236045671107No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC18490.21181368185791186No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18210.20860611934194564No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT17440.19978532242303856No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT16810.19256830676211456No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA16680.1910790813082731No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG15200.17412482229530882No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA15100.17297926425389232No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT14950.17126092719176755No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC14290.16370024411841863No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA14220.16289835348942708No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12610.14445486902262133No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12510.1433093109812048No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12410.14216375293978833No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG12270.14055997168180523No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA11480.13151006315461483No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC11410.13070817252562328No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC11380.1303645051131983No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG11290.12933350287592346No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT11180.1280733890303653No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT11030.12635505196824054No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG10900.12486582651439909No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG10610.12154370819429122No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA10150.1162741412037753No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA10020.11478491574993385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9640.11043179519255111No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9140.10470400498546859No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG8880.10172555407778569No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC8770.10046544023222753No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG8750.10023632862394423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19650.032.9516561
CGTCTTC7200.032.8888937
TTATACA21100.030.7748832
GATTACG551.8546416E-830.2727281
TATACAC22450.029.1714943
TTACGGG659.386349E-825.6153853
CCGTCTT22600.024.88495637
GCCGTCT22850.024.6126936
GTATCAA12100.024.3099171
GCGTCAA400.001930816723.12500226
GGTATCA4150.022.2891561
ATTACGG753.7386417E-722.22
TGCCGTC26250.021.42476335
CGTCAAA450.003825081720.55555527
TCGCCGA555.141733E-420.1818181
CGAACTA555.141733E-420.18181829
ATGCCGT27900.020.09139834
GTTATAT1254.110916E-1019.241
TATGCCG29450.019.03395733
CACGGTA1409.458745E-1118.52
Produced by FastQC (version 0.11.5)