FastQCFastQC Report
Fri 10 Feb 2017
ERR1630997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973492
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT285052.928118566973329No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG217052.229602297707634No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT176661.814704178359966RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC121231.2453106959276503No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC66400.6820805923417964RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC61120.6278428584929306No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG28090.28854885299519667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24200.24858961347396796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.24365891039679835No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA20580.21140389443364713No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT17850.18336052068224495No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13900.14278494327637004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC31650.034.0774081
TTATACA34050.031.6754782
TATACAC35250.031.0170213
CGTCTTC9200.028.75543637
TCGCCGA556.2600884E-726.909091
GATTACG556.2600884E-726.909091
CGGAATA706.5901986E-926.4285727
CGTTACG358.868416E-426.4285716
CCGTCTT26250.024.45523837
GCCGTCT26650.024.0881836
GTATCAA12850.023.8988321
GGTATCA4600.022.9239141
TTTACCG705.100599E-621.14285730
TTACCGG806.957871E-720.812531
GGACCGT909.47457E-820.5555576
GACCGTT951.675453E-719.4736847
ACCGTTA951.675453E-719.4736848
TGCCGTC33750.019.0207435
AGCCGCC801.616256E-518.513
TTGCGTC500.00703412618.537
Produced by FastQC (version 0.11.5)