##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630997.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973492
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422151389020147	31.0	31.0	34.0	28.0	34.0
2	31.45670020914399	31.0	31.0	34.0	28.0	34.0
3	30.944131025216436	31.0	31.0	34.0	27.0	34.0
4	35.064466888274374	37.0	35.0	37.0	32.0	37.0
5	35.2589543622341	37.0	35.0	37.0	33.0	37.0
6	35.45281933493033	37.0	35.0	37.0	33.0	37.0
7	35.44692098137427	37.0	35.0	37.0	33.0	37.0
8	35.470109667054274	37.0	35.0	37.0	33.0	37.0
9	37.25405344882136	39.0	37.0	39.0	34.0	39.0
10	37.07303193041135	39.0	37.0	39.0	33.0	39.0
11	37.12284024932922	39.0	37.0	39.0	33.0	39.0
12	37.063588606788755	39.0	37.0	39.0	33.0	39.0
13	37.14572487498613	39.0	37.0	39.0	33.0	39.0
14	38.25487215097813	40.0	38.0	41.0	33.0	41.0
15	38.25960151701298	40.0	38.0	41.0	33.0	41.0
16	38.23962497894179	40.0	38.0	41.0	33.0	41.0
17	38.20455740776504	40.0	38.0	41.0	33.0	41.0
18	38.25875610688121	40.0	38.0	41.0	33.0	41.0
19	38.28409581177863	40.0	38.0	41.0	34.0	41.0
20	38.31859224318227	40.0	38.0	41.0	34.0	41.0
21	38.27183068787417	40.0	38.0	41.0	34.0	41.0
22	38.18760092532861	40.0	38.0	41.0	33.0	41.0
23	38.19145406433746	40.0	38.0	41.0	34.0	41.0
24	38.123662033175414	40.0	37.0	41.0	33.0	41.0
25	38.11971233456464	40.0	37.0	41.0	33.0	41.0
26	38.01341973020836	40.0	37.0	41.0	33.0	41.0
27	37.94016591815855	40.0	37.0	41.0	33.0	41.0
28	37.947276402887745	40.0	37.0	41.0	33.0	41.0
29	37.86528702855288	40.0	37.0	41.0	33.0	41.0
30	37.80359674244883	40.0	37.0	41.0	33.0	41.0
31	37.790469772735676	40.0	37.0	41.0	33.0	41.0
32	37.66580002711887	40.0	37.0	41.0	33.0	41.0
33	37.59268900001233	40.0	37.0	41.0	32.0	41.0
34	37.56338624251663	39.0	37.0	41.0	32.0	41.0
35	37.54764291848315	39.0	37.0	41.0	32.0	41.0
36	37.56680075439757	40.0	37.0	41.0	32.0	41.0
37	37.51147210249288	40.0	37.0	41.0	32.0	41.0
38	37.47950162918647	39.0	37.0	41.0	32.0	41.0
39	37.415501103244814	39.0	37.0	41.0	32.0	41.0
40	37.28960279077794	39.0	36.0	41.0	31.0	41.0
41	37.26848500039035	39.0	36.0	41.0	31.0	41.0
42	37.22191861874571	39.0	36.0	41.0	31.0	41.0
43	36.517736149860504	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	19.0
11	27.0
12	18.0
13	13.0
14	15.0
15	16.0
16	16.0
17	17.0
18	24.0
19	39.0
20	80.0
21	127.0
22	371.0
23	635.0
24	1206.0
25	2077.0
26	3468.0
27	5424.0
28	8234.0
29	11979.0
30	16292.0
31	21872.0
32	28458.0
33	36837.0
34	49095.0
35	65397.0
36	90763.0
37	134737.0
38	231642.0
39	264584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.46480094340785	23.025767032497445	15.726991079536349	25.782440944558356
2	13.124709807579313	21.238489890004235	42.20507204989872	23.43172825251774
3	13.384188057015365	29.57456250282488	34.87147300645511	22.169776433704644
4	11.118427270075152	20.081726403504085	36.95572228636701	31.844124040053746
5	11.851766629823357	38.41397772143993	36.47117798605433	13.263077662682385
6	26.85199262038106	40.382252756057575	17.62839345366988	15.137361169891484
7	23.51329029925259	33.24413554502759	22.936398039223743	20.306176116496076
8	24.421258726317216	35.39402480965432	22.74810681546433	17.43660964856414
9	23.057097541633624	14.93869492507386	19.053058474029577	42.951149059262946
10	14.398063877258366	28.79427874086279	33.58979837533334	23.217859006545506
11	30.14631861381501	22.856685006142836	26.005247089857953	20.991749290184202
12	19.25922349644373	27.086406462508165	27.990985031207245	25.663385009840862
13	29.02951436683609	19.919732262822908	27.214604742514574	23.83614862782642
14	21.512349356748693	22.80224182633242	25.464616042042458	30.220792774876426
15	23.92972926331187	29.093202614916198	21.518101843672056	25.458966278099872
16	25.661535996186924	26.29163876025689	23.295003965107057	24.75182127844913
17	19.941920426670173	32.27956675555629	24.36116578256421	23.41734703520933
18	24.07313054447289	24.87180171999359	24.839033089126566	26.216034646406953
19	22.238189938900373	27.536127672338345	25.468005900408013	24.75767648835327
20	21.131247098075793	25.704782371092932	25.619419574069436	27.544550956761842
21	23.665833925702522	25.67848528801469	26.671200174218175	23.98448061206461
22	20.12651362312171	33.49529323302092	25.22444971299199	21.15374343086538
23	23.147493764715065	26.317833120354354	25.846026469657684	24.688646645272893
24	24.316994900831233	28.909431202310852	24.968155875959948	21.805418020897964
25	20.410953556885932	30.309545430265477	25.63534163608946	23.64415937675913
26	23.554584937523884	26.143101330057156	26.147723864192002	24.15458986822696
27	19.69322809021543	31.95270223073225	25.42506769444433	22.92900198460799
28	20.08182912648486	25.963952451586657	29.651502015424885	24.302716406503595
29	21.411680835589813	27.76704893311912	26.04849346476396	24.772776766527098
30	19.773249292238663	28.85026276538482	27.315067817711906	24.061420124664608
31	23.702814198781294	24.961581605190386	28.084873835635012	23.250730360393305
32	21.870852559651237	31.570983634174706	26.419528871320974	20.138634934853087
33	20.315010292842672	28.49340313017467	28.128531102464123	23.06305547451854
34	23.985302395910804	28.264639051990155	25.966417803125243	21.7836407489738
35	20.817428391810104	27.738183775521524	31.323113081566156	20.121274751102217
36	22.756838268830148	25.49800101079413	24.4036930966048	27.34146762377092
37	21.404901118858707	27.14485583856878	28.54753814104276	22.90270490152975
38	21.54285808203868	24.768976016238447	29.3079963677154	24.38016953400747
39	23.924182222350055	25.52501715473779	26.613778027965306	23.937022594946853
40	20.235297259761765	23.937536209850723	34.056982491895155	21.770184038492353
41	22.25113303447794	26.032571402743937	25.835856894561022	25.8804386682171
42	22.708763913827745	24.543190904496388	30.709754163362412	22.03829101831345
43	22.60727360882267	23.863267494750858	30.966767061259876	22.562691835166596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	17.5
2	28.0
3	76.5
4	125.0
5	125.0
6	171.5
7	218.0
8	223.5
9	229.0
10	358.0
11	487.0
12	487.0
13	755.0
14	1023.0
15	2506.5
16	3990.0
17	4520.5
18	5051.0
19	5051.0
20	5294.5
21	5538.0
22	6819.0
23	8100.0
24	10885.5
25	13671.0
26	13671.0
27	17189.5
28	20708.0
29	25575.5
30	30443.0
31	35304.0
32	40165.0
33	40165.0
34	45446.0
35	50727.0
36	55140.5
37	59554.0
38	64305.0
39	69056.0
40	69056.0
41	71147.0
42	73238.0
43	73388.0
44	73538.0
45	73720.0
46	73902.0
47	73902.0
48	74312.5
49	74723.0
50	94239.0
51	113755.0
52	84610.0
53	55465.0
54	55465.0
55	67865.0
56	80265.0
57	65049.5
58	49834.0
59	36850.0
60	23866.0
61	23866.0
62	20723.5
63	17581.0
64	14086.5
65	10592.0
66	8747.0
67	6902.0
68	6902.0
69	5490.0
70	4078.0
71	3419.0
72	2760.0
73	2298.5
74	1837.0
75	1837.0
76	1474.0
77	1111.0
78	825.0
79	539.0
80	391.0
81	243.0
82	243.0
83	169.0
84	95.0
85	61.5
86	28.0
87	21.5
88	15.0
89	15.0
90	9.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	973492.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.10030015080647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86981593080193	31.52041722922393
2	13.346013904955695	11.23742382430943
3	4.719300724890659	5.960519310594458
4	2.165891870609754	3.647387913874493
5	1.130851739980381	2.3804598839617896
6	0.7030394588277732	1.7758903340705883
7	0.4593576597870394	1.353736674752448
8	0.34140286381364215	1.1498530431119391
9	0.2704997167570864	1.0249307339563325
>10	1.6759864744926438	14.068124538795852
>50	0.20281521965860574	5.904096199879351
>100	0.1089449198121384	8.309756642668686
>500	0.0031613481185904786	0.8576353569637015
>1k	0.0014590837470417593	1.3139420044493428
>5k	4.8636124901391977E-4	1.305546863077608
>10k+	9.727224980278395E-4	8.190279446310035
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	28505	2.928118566973329	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	21705	2.229602297707634	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	17666	1.814704178359966	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	12123	1.2453106959276503	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	6640	0.6820805923417964	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	6112	0.6278428584929306	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	2809	0.28854885299519667	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2420	0.24858961347396796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2372	0.24365891039679835	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA	2058	0.21140389443364713	No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT	1785	0.18336052068224495	No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT	1390	0.14278494327637004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.0544596154873385E-4	0.0	0.0	0.0	0.0
6	2.0544596154873385E-4	0.0	0.0	0.0	0.0
7	2.0544596154873385E-4	0.0	0.0	0.0	0.0
8	4.108919230974677E-4	0.0	0.0	0.0	0.0
9	4.108919230974677E-4	0.0	0.0	1.0272298077436692E-4	0.0
10	8.217838461949354E-4	0.0	0.0	2.0544596154873385E-4	1.0272298077436692E-4
11	0.001129952788518036	0.0	0.0	2.0544596154873385E-4	1.0272298077436692E-4
12	0.001129952788518036	0.0	0.0	2.0544596154873385E-4	1.0272298077436692E-4
13	0.001129952788518036	0.0	0.0	2.0544596154873385E-4	1.0272298077436692E-4
14	0.001129952788518036	0.0	0.0	2.0544596154873385E-4	1.0272298077436692E-4
15	0.0014381217308411368	0.0	0.0	3.0816894232310073E-4	1.0272298077436692E-4
16	0.0015408447116155037	0.0	0.0	3.0816894232310073E-4	1.0272298077436692E-4
17	0.0015408447116155037	0.0	0.0	3.0816894232310073E-4	1.0272298077436692E-4
18	0.0015408447116155037	0.0	0.0	4.108919230974677E-4	2.0544596154873385E-4
19	0.0015408447116155037	0.0	0.0	4.108919230974677E-4	2.0544596154873385E-4
20	0.0015408447116155037	0.0	0.0	4.108919230974677E-4	3.0816894232310073E-4
21	0.0016435676923898708	0.0	0.0	8.217838461949354E-4	3.0816894232310073E-4
22	0.0016435676923898708	0.0	0.0	0.001129952788518036	3.0816894232310073E-4
23	0.0016435676923898708	0.0	0.0	0.00133539875006677	3.0816894232310073E-4
24	0.0016435676923898708	0.0	0.0	0.0016435676923898708	3.0816894232310073E-4
25	0.0016435676923898708	0.0	0.0	0.0017462906731642376	4.108919230974677E-4
26	0.0016435676923898708	0.0	0.0	0.0019517366347129713	5.136149038718346E-4
27	0.0016435676923898708	0.0	0.0	0.0032871353847797415	5.136149038718346E-4
28	0.0016435676923898708	0.0	0.0	0.009039622308144288	5.136149038718346E-4
29	0.0016435676923898708	0.0	0.0	0.02003098125100155	5.136149038718346E-4
30	0.0016435676923898708	0.0	0.0	0.03708299605954646	5.136149038718346E-4
31	0.0016435676923898708	0.0	0.0	0.08875265538905301	5.136149038718346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3165	0.0	34.077408	1
TTATACA	3405	0.0	31.675478	2
TATACAC	3525	0.0	31.017021	3
CGTCTTC	920	0.0	28.755436	37
TCGCCGA	55	6.2600884E-7	26.90909	1
GATTACG	55	6.2600884E-7	26.90909	1
CGGAATA	70	6.5901986E-9	26.42857	27
CGTTACG	35	8.868416E-4	26.42857	16
CCGTCTT	2625	0.0	24.455238	37
GCCGTCT	2665	0.0	24.08818	36
GTATCAA	1285	0.0	23.898832	1
GGTATCA	460	0.0	22.923914	1
TTTACCG	70	5.100599E-6	21.142857	30
TTACCGG	80	6.957871E-7	20.8125	31
GGACCGT	90	9.47457E-8	20.555557	6
GACCGTT	95	1.675453E-7	19.473684	7
ACCGTTA	95	1.675453E-7	19.473684	8
TGCCGTC	3375	0.0	19.02074	35
AGCCGCC	80	1.616256E-5	18.5	13
TTGCGTC	50	0.007034126	18.5	37
>>END_MODULE