FastQCFastQC Report
Fri 10 Feb 2017
ERR1630996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275455
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT167106.066326623223394No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG129434.698771124140059No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT125394.552104699497195RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC66582.4170917209707574No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC54121.964749233087074RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43231.569403350819553No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC39471.4329019259044127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39071.418380497721951No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22860.8298996206276887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16770.6088108765497087No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG15040.5460056996605617No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA14130.5129694505454611No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13340.48428962988509916No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12550.45560980922473726No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12160.441451416746837No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT12130.4403623096331524No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10110.3670290973117206No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9970.361946597447859No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9690.3517815977201358No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8970.32564302699170466No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8660.3143889201502968No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8550.3103955274001198No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8220.29841534914958884No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6840.24831642192009584No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6590.23924052930605727No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6380.23161677951026483No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6180.224356065419034No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5910.2145541013958723No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5840.21201285146394147No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5770.20947160153201067No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5650.20511517307727217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.20184785173621825No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5440.19749142328147973No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5300.19240892341761812No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5150.18696338784919497No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5110.1855112450309488No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4820.17498320959866404No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4650.1688116026211178No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4590.16663338839374853No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4560.16554428128006388No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4170.1513858888021637No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4120.14957071027935598No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4110.14920767457479442No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4000.14521428182461746No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3980.14448821041549437No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3900.14158392477900203No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3890.14122088907444047No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3760.13650142491514042No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3730.13541231780145577No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3570.1296037465284711No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3510.12742553230110182No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3490.12669946089197873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.12669946089197873No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3430.12452124666460947No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3380.12270606814180174No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3240.11762356827794014No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3240.11762356827794014No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3220.11689749686881704No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3210.1165344611642555No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3130.11363017552776317No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3120.11326713982320162No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3110.11290410411864008No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3070.11145196130039389No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3030.10999981848214771No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3000.10891071136846309No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3000.10891071136846309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2970.10782160425477846No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2890.1049173186182861No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2890.1049173186182861No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2890.1049173186182861No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2880.10455428291372457No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2880.10455428291372457No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2850.10346517580003993No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2830.10273910439091685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGT308.289042E-637.03
TATAGTT355.6106E-737.013
GTATACA200.001839507337.02
TTACGGG551.2732926E-1137.03
GACCGTT501.7826096E-1036.9999967
GCCGAGC251.2311901E-436.9999963
ACAGACA251.2311901E-436.99999617
CAGACAC251.2311901E-436.99999618
CGTCTTC6700.035.3432837
CTTATAC19600.035.1122471
ATTACGG603.45608E-1133.9166682
GATTACG603.45608E-1133.9166681
TAGGACC554.965841E-1033.6363644
CTTAGGA554.965841E-1033.6363642
CCGTTTA554.965841E-1033.63636427
CCGGGGC507.2886905E-933.334
TTACCGG507.2886905E-933.331
TTATACA21050.032.5178152
TTATAGT401.594226E-632.37512
ATAGTTA401.594226E-632.37514
Produced by FastQC (version 0.11.5)